Dear colleagues!
is it necessary to breed the female with vasectomised male for pesudopregnancy. As breeding objective is ovulation induction and preparation of uterus for carrying pregnancy. But if we go for hormonal manipulation and induce ovulation through hCG then this will have same effect as breeding with male or mating is essential.
The stimulation of the cervico-vaginal region (30 sec or longer) results in pseudopregnancy. You can use a wec cotton sab as the stimulus via the vagina.
Dear, Dr. Muhammad Ameen. I hope that your fine, and upon my modest experience. Yes, we can prepare pseudopregnant mouse without male mating via multiple( trial ) or repeated stimulation on vaginal compartment for about ( 20-60 ) second / once trail and for more than ( 5-15 ) stimulus / trail by using of a suitable oild bulb glass probe (smooth) or by using of oild cotton swab device. But, this have been done with those especially during at the estrous cycle period of mice.
Best Regards,
NASEER ALMUKHTAR
yes its possible by what mentioned above
The stimulation of the cervico-vaginal region (30 sec or longer) results in pseudopregnancy. You can use a wec cotton sab as the stimulus via the vagina.
Can we prepare pseudopregnant mouse without breeding with male? - ResearchGate. Available from: https://www.researchgate.net/post/Can_we_prepare_pseudopregnant_mouse_without_breeding_with_male [accessed Mar 10, 2017].
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