For some reasons, I need to use C57 or DBA mice with impaired immune system for organ transplant from other species such as Rat or monkey. SCID/NOD mice, however, can not be used in my exprements. I wonder that is there any medicine or small moleculars can disrupt mouse immune system totally to let the organs or cells from other species survive? I hope this medicine can inhibit the function of T cells,B cells and NK cells. Anyone has some good ideas?
What's about other mice strains like Rag1-/- or Rag2-/- maybe combined with common Gamma chain (gc-/-) knock out?
Hi Xiaoyu, cyclophosphamide has been used to suppress the immune system of mice.
http://jcb.rupress.org/content/jcb/122/6/1351.full.pdf
Thanks for the answer, it is worth to try cyclophosphamide in my experiment. I want to inject some Rat stem cells into the testis and the ovary of Mouse, however, I am worried that cyclophosphamide will also harm my stem cells. Will cyclophosphamide inhibit or kill the transplanted stem cells?
Hi Xiaoyu, cyclophosphamide is highly toxic and will undoubtable kill some of your transplanted stem cells.
This method was originally conceived to kill mature B and T cell in adult animals.
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