I'm interested in anesthetic-induced neurotoxicity and performing the experiment. In my experience, during anesthesia with isoflurane, respiration of mice is suppressed, and then PCO2 level increases and PO2 level decreases, and hypoglycemia occurred. Hypercarbia may induce the elevation of intracranial pressure, which may affect the brain function and tissue. Also, hypoglycemia and hypoxia may induce the brain damage. Therefore, it is important to maintain normocarbia (at least prevention of severe hypercarbia) and prevent hypoglycemia and hypoxia or marked bradycardia as well as prevention of hypothermia because we just want to know the sole effect of the anesthetics. But I did not find the relevant data in the several articles associated with anesthesia-induced neurotoxicity despite even the long time anesthesia of 6 hr. So it is quite difficult for me to accept the results of the articles. Do you agree with my viewpoint? If you have an experience or know the method which can keep the parameters despite a long time anesthesia in neonatal pups (e.g. a limited time of anesthesia), please let me know and discuss freely. Thank you.