Our lab, in Brazil, recently read the paper "Anti-inflammatory mechanism of lonchocarpine in LPS- or poly(I:C)-induced neuroinflammation". The authors say "Lonchocarpine (50 mg/kg) was dissolved in a vehicle solution (1% dimethyl sulfoxide [DMSO] and 0.9% sodium chloride) and administered via an intraperitoneal injection daily for 4 days".

We are currrently trying to prepare this solution. We understand that we should make a solution with a final concentration of lonchocarpine of 2.5 mg/mL in vehicle to administer a volume of 10-20 mL/Kg in mice (in order to administer 0.6 mL to a 30g mouse). But we are trying to vortex, heat to 38oC or sonicate and we still could not achieve this concentration (2.5mg/mL) in order to administer the dose of 50 mg/Kg.

Anyone knows how should we adequately dilute lonchocarpine to achieve this concentration? Should we dilute directly in vehicle or are there several preliminary steps to make the final preparation to be administered?

We tryied to dilute first in 100% DMSO and then dilute to 1%DMSO or 1%DMSO/1%Tween80 or 1%DMSO/2%Tween80, all in 0.9% NaCl.