I'm looking for a serum-free long-term (> 4 d) culture system for mouse granulosa cells. Protocols exist for sheep and cattle, but I cannot find one for mouse. Local colleagues simply say that it doesn't work in mice. Does anyone have any experience with this? What conditions have been attempted (I'd prefer not to repeat all the failed experiments a lot of people may have buried in their lab books!)?
Thanks!
www.sciencedirect.com/science/article/pii/S0890623814002305
The longest I try was 2days and by the end of two days, all cells were so unhappy. I even try low serum (1%) and they start to differentiate. Joanne s. Richards lab tried long term but with serum. Good luck
Notice the experiments of Prof. Dr. Katharina D'Herde ; Ghent University Belgium.
From ooplasms to embryo: The "embryo in box" model. Marc Callebaut. Edit. Acco Leuven/ Den Haag.
This book provides a unique, up-to-date overview of recent research on avian ovarian follicles and ensuing early embryonic development after fertilization.
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