i use ketamine/xylazine (110mg/kg and 25mg/kg) for sedation, buprenorphine administered at 0.1mg/kg before surgery and body temp maintained at 37*C during surgery. Most of the mice are dead within 20-30min of ischemia.

All the protocols that i have looked into recommend 2% isoflurane in oxygen, is it a good idea to completely shift to that.