The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
We used linear array CCD to capture movement object pictures. When extracting information from the pictured, we were bothered by the intensive sinusoidal stripe pattern (like the files jpg). What...
07 October 2019 6,177 1 View
What microscopy techniques were used to image the quantum dots?
01 October 2019 9,295 3 View
Hi, I am looking for a public dataset in "Single-cell microscopy - tissue diagnosis" like the data which is available in (https://www.nature.com/articles/nmeth.4391). In the context of Single-cell...
30 September 2019 8,351 1 View
Hi everyone, Usually finding the sample position in bright field is easy but when we change the microscop to the spectroscopy setting, my sample got lost. Does anybody has a solution for this...
16 September 2019 6,861 3 View
Hello experts from Research Gate, I am struggling with staining live E.coli with Hoechst 33342 and FM 4-64FX. The fluorescent images were too dim even at high concentration of the fluorescent...
06 September 2019 7,188 3 View
Dear Sir/Ma'am I want to know the effect of frequency within temperature range from room temperature to let say 250 C on loss modulus, storage modulus and glass transition temperature. Thanks...
24 August 2019 2,476 6 View
Hi, I'm working with a Leica SP8 confocal microscope, and I'm trying to analyze the sum intensity of z-stacks (16-bit). For particle analysis I'm making a max intensity image first which later I...
22 August 2019 2,259 6 View
I'm struggling to sort out an issue that all the sudden happened with a cleanliness inspector microscope.See the image attached. I've attempted the shading calibrations multiple times and it...
16 August 2019 1,759 3 View
Hello everyone, I'm trying to image neuronal tracts of specific neurons in the basal ganglia of an adult mouse, using viral retrograde tracers and light-sheet microscopy. Which method is...
09 August 2019 697 1 View
Question about transferrin receptor 1 (TfR1) trafficking. General assumption is that newly synthesized TfR1 is transported to plasma membrane by adapter protein complex-1 (AP-1). via Golgi. Is...
08 August 2019 1,258 0 View
I'm relatively new to microscopy and staining. I am working with a mouseline that is susceptible to developing early onset alzheimer. I see that often times papers reference staining myelin...
28 July 2019 7,398 2 View
Hi! Have anyone ever tried single protein tracking (let's say a secretory protein) but also see its relative position to other organelles such as ER and nucleus (labelled by other...
22 July 2019 4,297 3 View
An apparatus was constructed in the lab to measure charge carrier lifetime. - An LED illuminates an n-typed germanium semi-conductor with a square function with a constant frequency (the period...
17 July 2019 1,878 5 View
Hi there, I am trying to use ImageJ to automatically quantify filopodial density and length on HT1080 cell surfaces in scanning electron microscopy images. I have noticed that most of the...
11 July 2019 9,560 2 View
I am going to test hypothesis about before and after implementing a project, income of the beneficiary increases or not. I am doing Paired Sample T-Test for that. My data comes from random...
20 June 2019 909 8 View
I'm a technican in a biotech company, and my work is to purify lentivirus vectors for downstream clinical trials. I've been using anion chromatography→TFF→SEC accompanied by benzonase( I usually...
10 June 2019 453 0 View
I'm looking for a good protocol for taking fungi (mostly conidial ascomycetes) growing on an agar plate and fixing them and ultimately staining and mounting them. Would one simply remove a plug of...
04 June 2019 8,476 5 View
Hey, I'm looking for assays where I can track single cell - single bacterium dynamics, mainly rate of killing. Most methods I find online revolve around finding a single infected cell and...
29 May 2019 2,090 2 View
I’m facing a big problem with microscopy and would like to hear your suggestions. My aim is to visualize gamma-H2A.X foci in hameatopoietic stem cells under the microscope. When I try this or any...
28 May 2019 1,411 3 View
I have acquired some chep LED lights which apparently emith UV to NIR light (will need to measure it with a pyranometer in my setting anyway). I am trying to get a photocatalytic intrinsic...
21 May 2019 1,932 5 View
I am working on a new material so to understand its cytotoxicity I performed a MTT assay test. The sample showed a metabolic activity of 83%. So is this sample cytotoxic or bio compatible. Are...
08 May 2019 491 5 View
I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.
24 April 2019 9,030 4 View
There is a protein has two isoforms, one of which has a signal peptide. I am wondering if it is a secretory protein. If it is, how can I detect it? What if it is secreted by extracellular vesicles...
15 April 2019 2,881 0 View
What i mean is just as follow: The autophagy inhibitor,chloroquine, functions when autophagosome fuses to lysosome. As a result, use of chloroquine makes it blocked for LC3B-II to degrade in...
10 April 2019 9,948 4 View