The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
what is the apomorphy that make difference between Glucophytes and green algaes?
28 October 2018 4,409 3 View
I am looking for a way to visualize plasma membrane in mouse airway secretory cells, in paraffin sections by immunofluorescence. In other words, it has to be a sufficiently abundant in plasma...
04 October 2018 3,541 9 View
There is a protein has two isoforms, one of which has a signal peptide. I am wondering if it is a secretory protein. If it is, how can I detect it? What if it is secreted by extracellular...
20 September 2018 1,490 2 View
During the charging phase of a lithium ion cell I found some small voltage drops (as shown in the attached figure). Can you tell me what they're due to?
03 September 2018 4,398 2 View
I have just learned photoacoustic imaging for less than one week. I am confused about some issues of optic-resolution photoacoustic microscopy (OR-PAM) and acoustic-resolution photoacoustic...
03 September 2018 4,491 3 View
I want to couple NP to PMSs so that it can be recognized by NP-spec. B cells.
21 August 2018 2,054 2 View
I am trying to setup an automated pipeline to acquire images of individual neurites in a sample. For this I am using the NIS elements software. I have both DAPI and Phalloidin available as...
08 August 2018 5,174 3 View
Greetings dear scientific community, Today I would like to ask a question which is partly related to my work. I have been doing microscopy as part of my job for four years now, and I always had...
02 August 2018 9,166 4 View
Just want to know if red blood cells could be detectate in far red (650-750nm wavelenght) under 514nm laser excitation.
27 July 2018 1,042 3 View
I am working on Symbiodinium cells (symbiotic dinoflagellates) and am trying to calculate the mitotic index. All the papers I have referred to use DAPI. I want to know if there are other stains or...
15 July 2018 150 3 View
While recording high speed images of MCF7 cells flowing in a microfluidic device, I've observed that some cells appear much brighter than the rest of the population. I've read that cells, just...
28 June 2018 1,956 2 View
Hi there, I have a stock solution of a clinical isolated phage with a titre of ~3E12 pfu/ml. Is it high enough for TEM microscopy, and is there any other considerations for imaging? Regards Alireza
24 June 2018 9,261 2 View
I am trying to distinguish the pillar cells from thin and thick epithelial cells of Chinese mitten crab so that I can study using pH dyes regulatory capabilities of the individual cell types....
14 June 2018 556 2 View
I have taken a Z-stack of a cell using a Zeiss confocal microscope. I have orientated the image in 3D and need to include a scale bar/take measurements in this orientation. Does anyone know how to...
27 April 2018 5,170 3 View
I have more than 10k images (Phase contrast, 10X) of hMSCs and I need to count the cells. Right now, I am using ImagaeJ jo manually count them in a 'tick-tick' fashion which is very laborious and...
25 April 2018 4,285 5 View
under microscopy how differentiate between the THP1 and Macrophages cells?
06 April 2018 6,163 3 View
We are using Leica DM5500b microscope to do our microscopy on Giardia intestinalis. However, despite the fact that around 3 years ago we were able to obtain amazing DIC pictures, the DIC pictures...
31 March 2018 4,640 3 View
The idea is to visualize by microscopy the arrival to the surface of the plasma membrane and to determine the time until the protein is endocyted again. Thanks.
15 March 2018 3,237 2 View
what is the energy band change of mesoscopic perovskite solar cell using scanning Kelvin probe microscopy
09 March 2018 978 3 View
Does any experience in capturing images to analyse microtubule dynamics in cells (eg track end binding protein movements on individual microtubules) without using spinning disc microscopy? How did...
26 February 2018 3,806 2 View
I want to decide on choosing a high intensity illumination source for my microscope. My default choice is a 130W mercury lamp but I've heard that brighter LED lamps also exist and if that is the...
23 February 2018 2,704 3 View
For clear(non-convertor phospher) encapulation SMT technology LED, when single unit gets illumination value degradation, how to process FA to get the root cause?
23 February 2018 8,187 0 View
Protein isoelectric point IMAC
22 February 2018 1,173 3 View
For dielectric measurement, ferro-electric testing and Piezo force Microscopy.
20 February 2018 4,289 2 View