Which clearing method is recommended to image axons in mouse brain?

09 August 2019 1 495 Report

Hello everyone,

I'm trying to image neuronal tracts of specific neurons in the basal ganglia of an adult mouse, using viral retrograde tracers and light-sheet microscopy. Which method is recommended for tissue clearing in this case?

Nimrod

Kundan Das

Nimrod Bernat For mouse brain axons, I would recommend trying focusclear. It is especially effective in mouse brain, see here for reference: https://bit.ly/2lVOqdL

Hope this helps!

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