Culture Techniques

Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs;... | Contact experts in Culture Techniques to get answers

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Questions related to Culture Techniques

How can I calculate the required volume of gas for a closed culture system for mammalian cells?

I need to culture mammalian cells on a 60mm culture dish in an airtight container with 5% CO2 gas (5mL growth medium). Are there any rules / published data on the ratio of medium : atmosphere...

01 January 2020 6,230 3 View

How to culture Entrobious vermicularis ova in lab?

I am trying to culture Entrobious vermicularis ova in normal saline and nutrient agar. The ova is isolated from pereanal area of entrobious positive childern by Adhesive Tap Technique. Anyone...

10 October 2019 3,234 1 View

How to culture Entrobious vermicularis ova in lab?

I am trying to culture Entrobious vermicularis ova in normal saline and nutrient agar. The ova is isolated from pereanal area of entrobious positive childern by Adhesive Tap Technique. Anyone...

07 October 2019 5,719 1 View

How to culture cancer cells the right way?

I am relatively new to cell culturing and I am currently working with several lines of cancer cells, some of them being HeLa, A549, HCT116, etc. I have some very basic doubts regarding the...

09 September 2019 8,627 3 View

How to culture cancer cells the right way?

I am relatively new to cell culturing and I am currently working with several lines of cancer cells, some of them being HeLa, A549, HCT116, etc. I have some very basic doubts regarding the...

04 September 2019 3,142 3 View

Are hWJ mesenchymal stem cells easily culturable?

Hello I was wondering if it is easy to culture human wharton's jelly mesenchymal stem cells ? does anyone have any experience with these cells?!

08 August 2019 8,257 2 View

Gelatin as a gelling agent for fungal growth media?

I'm planning an experiment involving imaging cultures of mycoparasitic Trichodermium interacting with various species of host fungi and am thinking of various methods of imaging the interaction...

27 May 2019 2,603 3 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

30 April 2019 5,202 3 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

22 April 2019 2,841 1 View

Is there a system to grow large numbers of Plasmodium gametocytes with reduced labour?

Hello! I am researching alternative methods to grow large numbers of Plasmodium falciparum gametocytes (for mosquito infections) which are not the traditional tissue culture flasks, and so can be...

04 April 2019 7,651 1 View

How determinate the incubation time for a culture of microorganisms ?

There is a question surely simple but for which I never found answers, when a couting is realized after growth on petri dish, how is determined the incubation time in order to demonstrate the...

03 March 2019 8,031 1 View

How do make a glycerol stock of bacteria for future use?

How do make a glycerol stock of bacteria for future use? I need valuable suggestions for that.Please put here and share your experience.

26 February 2019 9,432 3 View

Is it possible to adapt the RPMI-1640 medium for the cultivation of HEK-blue cells?

Hello I am a master student, I want to work with HEK-blue cells (cells derived from transfection of hek 293 cells, these cells are developed by invivigens). According to the supplier's...

16 February 2019 2,500 2 View

Does someone share me an easy culturing technique for Pratylenchus species other than the carrot disc?

Pratylenchus is an obligate biotroph

06 February 2019 9,693 8 View

Is it possible to adapt the RPMI-1640 medium for the cultivation of HEK-blue cells?

Hello I am a master student, I want to work with HEK-blue cells (cells derived from transfection of hek 293 cells, these cells are developed by invivigens). According to the supplier's...

02 February 2019 2,103 3 View

Does someone share me an easy culturing technique for Pratylenchus species other than the carrot disc?

Pratylenchus is an obligate biotroph

02 February 2019 4,494 9 View

Why does my freeze-dried culture of Carboxydothermus hydrogenoformans (DSM6008) not revive?

I have tried to revive the above organism from a freeze dried culture provided by DSMZ. As is usually the practise, instead of utilizing the entire culture at one go, we use sterile loops to...

02 February 2019 8,086 12 View

I'm looking for a rare article about axenic cultural technique ?

Ishii T., Horii S. (2007). Establishment of axenic cultural techniques of arbuscular mycorrhizal fungi. Hort. Res. (Jpn) (Suppl. 2) 6, 136. Anybody can find this article ??? One thousand of...

25 January 2019 3,908 1 View

I'm looking for a rare article about axenic cultural technique ?

Ishii T., Horii S. (2007). Establishment of axenic cultural techniques of arbuscular mycorrhizal fungi. Hort. Res. (Jpn) (Suppl. 2) 6, 136. Anybody can find this article ??? One thousand of...

01 January 2019 5,473 1 View

Are the exosomes isolated with commercial kits from cell culture media, suitable for using in cell treatment studies? Has anyone already did it?

Hi, everyone. I aim to treat some KC lineages with extracellular vesicles (EVs) from culture media of another treated cells (these ones simulating a pathological state). We have a commercial...

03 October 2018 6,921 7 View

Can someone help with culturing techniques of Staurastrum sp, Arthrodemus sp, Staurodesmus sp?

I isolated and am culturing Arthrodemus, Staurodesmus sp, Staurastrum sp using BBM but am yet to see results. Can someone help me with the specifics? Thank you.

09 September 2018 7,490 0 View

Which is the best 3D model human adipocytes culture techniques ?

Hello, Dear Scientist I am just wondering about the using of 3D model for a better differentiation of preadipocytes on the mature adipocytes. May I ask you, If there is somebody, who could help me...

07 July 2018 300 3 View

What is the fastest technique for soil bacteria identification?

I wanted to perform a quick experiment (10-25 minutes) which will help me to check microbial presence in soil, I have found many culture techniques but those at least take 8 hours of duration, I...

02 April 2018 4,143 9 View

Has anyone observed loss of global DNA methylation in HuLy or Tk6 cells without treatment - either due to wrong culturing or during DNA extraction?

I am working in cell culture using Tk6 cells and primary cells (human lymphocytes). Analysing the global DNA methylation with an ELISA I observed only 0.1-0.2 % of global methylation status....

23 January 2018 594 1 View

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