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Questions related to Molecular Methods
Enteric microbes are often identified in drinking water using simple laboratory techniques. What will be the importance if one choose to isolate these microbes and further suject them to PCR or...
12 November 2020 10,212 3 View
I am working on Chlamydia trachomatis characterization and I would like to use molecular methods rather than serologic method. Can anyone provide me with the protocol for identification of...
18 July 2020 3,244 1 View
If one needs to compare the expression levels of a particular gene experimentally (molecular method, not omics approach), can there be a reliable method to measure it? What kind of control can be...
12 December 2019 7,918 3 View
03 December 2019 6,712 3 View
pest monitoring
10 October 2019 8,671 1 View
Molecular methods represent the most accurate and rapid methods for detection of bacteria which give rise to Infectious diseases. The WHO approved LAMP method has been successfully used in...
01 January 2019 6,863 3 View
Microscope examination Immunological method ( Trichomonas Rapid test) Culture method Staining method pH measurement Whiff test using Hematology Analyzer for an account pus cells molecular...
17 November 2018 6,008 14 View
There is a number of collected samples from individuals that visited local clinics for routine check-up. Along with demographics available are also some epidemiological information, regarding pet...
11 November 2018 6,459 11 View
11 November 2018 10,094 16 View
I'm doing prevalence study of Toxoplasmosis using both serology (ELISA, Platelia Toxo IgG & Platelia Toxo IgM kit, BioRad USA) and molecular method (PCR). During ELISA assay, my samples were...
11 November 2018 4,895 12 View
08 November 2018 8,277 10 View
depending on your experience which method is the best?
10 October 2018 6,298 3 View
I want to create a serial dilution of genomic DNA and plasmid samples for absolute qPCR. In order to get repeatable results in the Real time PCR, I want to do a very accurate dilution. I know that...
10 October 2018 7,579 13 View
Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?
09 September 2018 5,501 0 View
Currently, I am trying to determine the population genetic structure of two species of nudibranchs from the Caribbean. Previous research has been done with tradicional molecular methods and this...
09 September 2018 3,194 4 View
Particularly bacteria,fungi and viruses.
08 August 2018 4,154 2 View
I have to characterize the elite plant lines producing secondary metabolites. Any body please recommend the suitable and viable method?? thanks
07 July 2018 960 0 View
how we can remove of antibiotic resistance gene-carrying plasmids from bacteria such as lactobacilus with molecular method ?
07 July 2018 5,377 1 View
Dear all, I have a situation and need someone help to find out. I need to know microbes that cause swollen packs of UHT chocolate milk. Currently, we do not know a specific microorganism cause...
06 June 2018 7,242 0 View
i want to carry out a project in crime forensics. this will help as a medium to my country adapting to the molecular method of dealing with crime cases
05 May 2018 2,212 4 View
How can I distinguish S. cerevisiae var. boulardii from other S. cerevisiae strains (preferrably by molecular methods)?
05 May 2018 7,815 0 View
Does anybody know which molecular typing methods would be the best for checking if the strain of S. cerevisiae is boulardii or not? I can't determine microsatellites polymorphism due to a lack of...
04 April 2018 5,104 2 View
03 April 2018 4,165 2 View
Hello researchers Can somebody please , provide me the recipes to prepare buffers that used with silica spin column and the protocol to isolate the genomic DNA from blood . best regards
03 March 2018 1,577 4 View