The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
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any research about nutrition education among university student
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Alteast three issues.
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I am looking for feedback.
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Hello Everyone, please Can I get possible research topics for solar cells and separation membranes?
09 February 2023 475 2 View
Hello We implemented a small-sized VRLA (AGM Valve Regulated Lead Acid) battery project on the number of 10 ethers. In these batteries, sulfuric acid with a density of 1.250 and each battery cell...
19 January 2023 4,456 0 View
This diatom was collected from the half-salt water in Macau. This is a SEM figure.
12 January 2023 1,136 0 View
this cell is iPSC derived mesoderm. I dont know what makes my cells diying... please tell me What kind of phenomenon does it look like during the process of dying? I'd like to get a clue as to why.
27 December 2022 3,660 1 View
I want to know how to check the health status of the cell lines under the microscope
12 November 2022 2,554 2 View
I am generating fibroblasts from healthy donors and used them at early passages. Some of my colleagues lost their cultures after serum starvation prior the start of an experiment. Can overnight...
11 November 2022 4,428 1 View
Dear forum users, I am trying to automate the generation of ROIs on a fluorescence time lapse image of SH-SY5Y cells to quantify potassium flux. We aim to calculate baseline response,...
07 October 2022 6,578 1 View
I should stimulate a neutrophils culture to undergo apoptosis as a positive control for Annessin V kit. Which is the best way to induce apoptosis in a short time? Thanks
21 September 2022 3,099 1 View
A549 cells were attempted to be drug-resistant by exposure to drug treatments for a total of 5 months. After the applications, the cells were tried to be removed by trypsinization but could not be...
20 July 2022 6,108 3 View
Hi all, Optical microscopy resolution limit is explained by Rayleigh criterion (widely applied). We have noticed that some papers show that Saddle-to-peak ratio should be 81.1% if the structure...
09 July 2022 7,234 0 View
I'm working on the antimicrobial activity of microbial metabolite on pathogenic bacterial strains. Can anyone tell me any alternative to SYTO9 fluorescent dye to observe live strain in fluorescent...
22 June 2022 3,473 2 View
Can a microscope equipped with a spinning disk Scan a Sample by the use of a reflection modality?
01 April 2022 2,068 0 View
Hey everyone! Have anyone faced an issue of how to minimize/quench autofluorescence in mammalian live cells? To be more specific I always stained and visualized ovarian cancer cell lines in blue,...
18 February 2022 2,449 0 View
We are performing cell fractionation to separate several organelles + cytosol + plasma membrane- We then want to isolate peripheral proteins from plasma membrane. We have tried increasing pH (up...
07 January 2022 1,472 0 View
I have been doing a subcellular fraction of Nucleus, Mitochondria, and ER in the HEK293 cell. But in results, I usually see all the three fractions have an equal amount of protein. Please help me...
20 December 2021 4,613 2 View
Hi all, Does anybody know how to segment the cytoplasm of cells using imageJ but excluding the nucleus (creating a donut-type selection). I would like to use this to create an region of interest...
04 August 2021 893 3 View
My two intependent variables are IFN-y and IL-2 T-cell responses to a given antigen. My dependent variables are 10 different antibody titers for that antigen in serum and saliva. I want to analyze...
22 July 2021 4,044 2 View
If possible please let us know about the cell type and the flurophore/dye used. Thanks. https://www.thermofisher.com/order/catalog/product/A50299?SID=srch-hj-A50299#/A50299?SID=srch-hj-A50299
23 June 2021 8,277 1 View
I have made a medium with agarose instead of agar - agar. Can bacteria also grow on agarose ?
27 May 2021 7,175 2 View
Hi, I am trying to measure the change of the refractive index (RI) of my sample by measuring the OPD with a QWLS wavefront senor. For this aim I have two different setups. My first setup is the...
07 April 2021 8,143 4 View