PCR, Cloning, Restriction Digestion, Ligation, Transformation, Plasmid et al | Contact experts in Molecular Biology to get answers
1,469 views 2,910 posts
Questions related to Molecular Biology
I am looking for papers with detailed methods on what molecular biology techniques are available for engineering mushroom-forming Basidiomycetes. I know that they are more difficult to manipulate...
27 September 2021 7,865 4 View
What exactly we need to build a molecular biology lab from scratches? What are the basic minimum requisites? What was required 15 years back is now obsolete. What will we require to study the...
09 September 2021 4,666 5 View
We could not get the data that Genclone uses to draw the Pareto chart. We want to draw a high-quality figure of the graph because the software produces low-resolution figures that do not meet the...
29 June 2021 2,860 2 View
I am planning to have endonuclease Ⅳ and Bst/Taq work in the same buffer, but definitely in different Temperature, i.e, the system will be passing first 37℃ then 60/95℃. Can I simply put...
14 May 2021 3,212 3 View
I am creating CRISPRi tool for Lactococcus lactis. Do I need to add an extra Lactococcus Terminator sequence at the end of the sgRNA or is the S. pyogenes Terminator enough?
28 April 2021 9,394 3 View
Fully funded Post-doc looking for host lab. I've a PhD in biotechnology and molecular biology. I need approval letter to submit. For cv DM on [email protected]
24 April 2021 9,836 4 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
02 March 2021 8,544 2 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
20 February 2021 7,221 5 View
Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...
17 February 2021 2,692 7 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
13 February 2021 1,586 3 View
I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...
10 February 2021 5,363 5 View
I am searching for tools to construct molecular beacons which shall be complementary to a given sequence. I don't want to design any primers. I found that UNAfold is one such tool which I could...
03 February 2021 9,233 5 View
Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...
27 January 2021 4,244 3 View
Hello everyone, we are acquiring a new molecular biology laboratory with the fundamental objective of identifying species of microalgae and cyanobacteria. Perhaps you could help us decide if it is...
25 January 2021 8,500 4 View
Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...
20 January 2021 6,053 2 View
Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...
15 January 2021 4,559 8 View
I made a new bacterial strain with an unmarked deletion using 2 step homologous recombination (with sacB counterselection). The full protocol is given in the link at the bottom. The protocol...
02 January 2021 1,769 4 View
Since two decades we came across many tools to edit the desired gene and genome viz., clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 (Cas9), (2)...
28 December 2020 7,783 10 View
Right now, I want to design qPCR primer for my target gene. But that gene has 21 predicted mRNA sequence in the NCBI database. Most of the sequence names are same but only different location...
24 December 2020 5,675 12 View
Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...
23 December 2020 5,428 5 View
It is known that the Uracil-DNA Glycosylase can remove the basic residue of dU. Thus the DNA chain will further cleave at the point of dU when heating to 95℃ However, is there any method to cleave...
19 December 2020 7,064 3 View
Dear all, My question is about Inducible hypoxia in vivo (mouse model). I'm looking for some chemicals that can induce the pathway of hypoxia or some other techniques. If you now know something...
16 December 2020 1,013 6 View
I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...
14 December 2020 5,847 5 View
I was using molecular grade water for the qPCR experiment. I want to know if I use autoclaved double distilled water is it fine? What about using DEPC water? Please share your knowledge and...
14 December 2020 3,344 14 View