The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
1,846 views 543 posts
Questions related to Microscopy
The experimental set up requires two cameras to be fitted to an olympus scope, one for fluroescence imaging, one for IR-DIC. We have the two cameras in mind, but need to fit them both to the same...
01 January 2016 3,119 1 View
I store a mouse brain in PFA 4 % and Sucruse 30%. I fixed the brain in agarose 3.5 % and cut the mouse brain at vibratome. The samples have a 100 micrometers of thickness. I put the five samples...
01 January 2016 7,549 3 View
research papers on processing Dunaliella tertiolecta for EM study.
01 January 2016 9,962 2 View
In a recent paper (http://elifesciences.org/content/early/2015/11/28/eLife.10396), it is claimed that mCherry can quench itself at high (local) concentrations. I have tried to find information...
01 January 2016 521 10 View
I am working with inhibitors of adenovirus replication and I would like to see what is the effect, if any, of these compounds on the replication center.
01 January 2016 6,310 1 View
How to calculate the grain size of following images.
01 January 2016 7,080 5 View
I am doing some cross-sectional imaging with a light microscope, and I've run into a problem: Some of the fibers in what I'm looking at are clear, and so is my embedding medium (a kind of paraffin...
01 January 2016 7,599 4 View
I am looking for a wideband powerful monolayer LED in order to substitute the filament lamp in a microscope, any suggestions? Thank you.
22 December 2015 4,580 5 View
Thanks
10 October 2015 4,562 1 View
For preparing IHC slides of rat brain sections, we mount with Fluoromount G, but it is common knowledge in the lab that over time it lets in air bubbles. Does anyone have any tricks to prevent or...
07 October 2015 2,194 3 View
Hi, from many scientific articles (example, http://journals.aps.org/prx/abstract/10.1103/PhysRevX.5.021014), I see that when you focus the longitudinal dipole on pinhole (for confocal setup), its...
09 September 2015 6,333 2 View
Is there any plugin or macro to remove the air bubble in the image? Thank you very much.
09 September 2015 1,105 1 View
If any protocols are available it would be helpful. i have also attached two Fe-SEM images.
09 September 2015 2,547 5 View
It is very easy to setup a 4F Fourier filtering setup with two converging lenses (we just place them apart by sum of their focal lengths) and use back & front focal planes (of 1st and 2nd...
09 September 2015 8,177 2 View
We have a "Leica M205 A" with a camera in our lab, which is used to photograph small animals and animal parts for taxonomic and systematic studies. We have been using black paraffin as background...
09 September 2015 8,246 3 View
I am looking for a rapid method for evaluation 900 microscopic plant tissue samples. this is my traits under evaluation: in stem: epidermal cells; collenchyma cells; cortex cells; cortex;...
08 August 2015 2,544 4 View
Some microscope objectives offer long working distance, low magnification, and moderately high NA (say 0.5) for the purpose of imaging wide fields of view. There are aspheric lenses which have...
08 August 2015 1,875 5 View
Dear collegues, your help is needed. In our lab we cover slides manually. Now with use Canada Balsam by Panreac (https://www.applichem.com/en/shop/product-detail/as/canada-balsam-dc) After...
08 August 2015 1,358 3 View
I want to image a diffuser (e.g. 1500 grit glass diffuser). Is it possible to do so using a microscope objective and normal (say aperture f/8) lenses? When i say imaging a glass diffuser, it means...
08 August 2015 3,098 7 View
I thought endosome membrane is derived from the plasma membrane of the cell, what is the difference that is allowing digitonin to have this effect?
29 July 2015 3,043 1 View
Hello! We've recently received the Cystic Fibrosis Manchester epidemic strain of P. aeruginosa. On first look under a microscope, we've seen a very unusual morphology (chains rather than single...
07 July 2015 681 5 View
I'm working on a new polyhexamethylene-guanidine hydrochloride-based biocide and testing its toxicity on human cells. I want to detach cells following their exposition to this substance in order...
06 June 2015 4,842 8 View
I'm looking to 'deroof' hMSCs so that I can image focal adhesion structures with force microscopy techniques and have seen multiple techniques and wanted to see if anyone has done this recently.
06 June 2015 5,391 0 View
I'm trying to use FIJI to make a kymograph where i can follow fluorescence during time and cell cycle. Is anyone able to explain to me how I can get this kind of picture ? Every tutorial I was...
06 June 2015 6,819 12 View