The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
Hi, Could you please share your experience regarding upsides and downsides of these microscopes? Which micrsocope is better for fluorescent imaging in fixed cells at 100x?
01 January 2017 7,473 1 View
Hello! I'd like to capture some digital photographs of co-infiltrated tobacco leaves expressing a GFP reporter. Our lab uses a Nikon Coolpix P80 camera. If you have suggestions on a way to mount a...
01 January 2017 2,255 3 View
I have an old Olympus IX70 inverted fluorescence microscope and need to get a copy of the manual for its fluorescent light source (it is a separate manual from the microscope manual). I had a copy...
01 January 2017 8,132 2 View
We suspect from the contamination so is the pictures (Orange arrow) contamination or something else?
01 January 2017 6,737 4 View
Hello! I have a question regarding the quantification of data obtained from microscopy. It is clear that if you take several images from the same organ/tissue, you can not consider one picture as...
21 November 2016 7,449 3 View
11 November 2016 1,627 4 View
Just read this on the data sheet, but I am not a biologist, so I am aking to understand more.
10 November 2016 7,826 3 View
I want to measure intracellular cAMP in various cells. Which simple method is available? I read some papers about EPAC BRET, but I am looking for a SIMPLE method. I have a decent microscope with...
26 October 2016 9,337 10 View
10 October 2016 8,378 11 View
For my masters project I need to extend an existing setup to be able to measure the phase of the recorded intensity field. My supervisor proposed to use an off-axis holographic method to obtain...
10 October 2016 3,119 4 View
We want to use a Zeiss Hyrax M40 microtome for plastic sectioning. It has never been used for plastic, and we have Leica Diamond blades that we were thinking of using. Any suggestions whether this...
10 October 2016 249 2 View
Hello, dear researchers, I want to ask that I am using the system as shown in figure 2. I performed an experiment and I measured total reflected intensity from the CCD as shown in figure 1(a). I...
10 October 2016 840 7 View
Cells are 3-5 μm and the mucilage is red. Collected in western Canada
07 October 2016 164 15 View
05 October 2016 5,280 2 View
Hi All, I am working on pathogens damaging water hyacinth, and during the morphological identification in the microscopy unit, I got these pictures which are attached as well as the original...
09 September 2016 477 7 View
Please suggest procedure for slide preparation. Thank you.
09 September 2016 7,098 0 View
Can I autoclave media to sterilise after having adjusted the pH (5, 6, 7, 8, 9)? Carrying out experiment involving growth under different pH ranges, but must be sterile and don't have access to...
09 September 2016 4,780 4 View
I want to meet more experts of microscopy.
09 September 2016 6,191 0 View
Hi, I would like to know what is the most efficient way to observe the spores of Ganoderma ? I have tried a few method to collect the spores from Ganoderma but sometimes the microscopic view is...
09 September 2016 8,118 4 View
I'm using a Nikon Eclipse microscope, and I wanted to make sure that images from different objectives (e.g., 10x, 40x) would be centered at the same stage position. Strangely, they were centered...
09 September 2016 8,035 5 View
We got an old Nikon C1 confocal mounted on a TE2000-E inverted motorised microscope. The lasers are 408, 488 and 543 nm. They not so well aligned to the coupling fiber. Intensities are oscillating...
09 September 2016 4,205 0 View
If the limit of an optical microscopy system is to resolve the finest element in group 7 of the resolution charts, which has the width of ~2.19um. Does this mean the resolution of the system is...
09 September 2016 2,011 5 View
The resolution of light microscope must be understood.
08 August 2016 3,048 4 View
We are interested at looking for a protein complex that may bind DNA. The protein of interest doesn't appear to directly bind DNA (doesn't have a predicted DNA binding sequence) but localizes to...
08 August 2016 2,308 2 View