The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
Say you have a calcium ionophore, which would normally transport Ca2+ across the plasma membrane to increase cytosolic [Ca2+]. Now suppose you have a Ca2+ free buffer but still treated with a...
10 June 2017 3,826 8 View
Immersion oil has gotten all over the body of our Nikon Eclipse Ts2R and it won't come off. Lens cleaner takes care of the objective lens, but the rest of the knobs/base/etc. is tacky, dirty, and...
06 June 2017 4,934 3 View
Autophagy-related proteins that could be stained or measured?
06 June 2017 2,566 2 View
We want to conduct immunogold labelling for scanning electron microscopy. As this is our first experience, I would like to know how long the samples can be kept in the fixation solution after...
31 May 2017 8,386 3 View
I'm trying to measure the cross section of collagen fibers using Bruker's nanoscope analysis software. I know, I have to flatten the image and then need to use section or step to get an output....
29 May 2017 4,164 1 View
I often find these items (marked with red arrows) while doing equine FECs. I am interested to know what they are. I am pretty certain that they aren't parasite eggs, and I find them in samples...
24 May 2017 4,848 9 View
We are interested in adapting a Confocal microscope for long-term live imaging, and for this reason we are trying to get a small environmental chamber at a reasonable prize. It could be enough...
17 May 2017 5,608 2 View
Does anyone have experience dealing with 3D tracking of microscale system, especially those can be embedded into microscope? My working system is at microns, so I don't need super resolution. One...
08 May 2017 5,118 2 View
I am working on a dissection fixation protocol for a non model organism and I am using DM1A alpha tubulin as a control to see if immunos work with a given trial of protocol. I think I got to a...
07 May 2017 4,208 2 View
Hi, I am trying to source an LED module suitable for insertion in the side port (ca. 8 mm diameter) for coaxial illumination in my microscopy setup. I have searched the sites such as Thorlabs and...
26 April 2017 2,063 2 View
Any ideas on how to couple a laser source to a infinity corrected microscope would be appreciated
25 April 2017 906 2 View
Hello. I am looking for something to stain brain cryosection tissue that is visible in normal light microscopy, but does not yield a significant signal with fluorescence microscopy (in range 405...
25 April 2017 645 3 View
Hello all, I have stained some bacteria using Gram staining process i.e. Gram Positive are stained with crystal violet and Gram negative are stained with sefranin. My aim is to see bacteria at...
24 April 2017 6,949 4 View
I usually use PFA 4%. However I must fix cells (Hs 578Bst) with a not hazard-toxic compound. I thought to use air flow comes of biological cape, but the morphology must be affected. Some...
20 April 2017 488 4 View
I'm trying to get better pictures of yeast mitochondria and the facility I have access to has a choice of spinning disk, Olympus FV1000 MPE, Olympus FV1000 - two photon, and Leica SP8 MP. I think...
04 April 2017 7,771 2 View
I recently received the revision from one of the journal and they asked me to do the corelation analysis of the protein into the membrane. However, I stained the protein glucocorticoid receptor...
04 April 2017 490 0 View
Magnet objects is very danger for electron microscope. Is is possible to get SEM images ? Thanks
03 April 2017 3,776 6 View
Does anyone know if Calcein AM assays have been successfully used on anaerobic cells to measure cell viability/ cell proliferation? A link to literature supporting your answer would be greatly...
03 April 2017 5,554 0 View
Greetings, here is friday morning! Nothing but, there is a question as to the microarray. I use Arrayit micro spotting solution 2X plus. As you can see in the photograph, after baking recommended...
31 March 2017 6,820 7 View
All "normal" lymphoblastoid cell lines (LCLs) I am aware of are transformed using EBV. EBV-negative B-cell lines I know of usually originate from cancers, e.g. Burkitt lymphoma. I am trying to...
28 March 2017 3,319 2 View
Separation of epoxides using a B-bex column with acetonitrile as solvent and deterctor FID. The conditions are: oven temperature-max 70 ° C, injector 280 ° C, detector 220 ° C, pressure 38 psi. I...
27 March 2017 2,902 4 View
I have data on whole skeletal muscle tissue concentration of phosphatidylcholine (PC) and phosphatidylethanolamine (PE). I do not have data on subcellular concentrations of PC and PE. Still, I...
16 March 2017 1,885 2 View
What can I use to test the performance of a Zeiss 880 Airyscan with PALM super-resolution? It is supposed to have 140nm Airyscan resolution and 20nm super-resolution. I'm looking for something I...
14 March 2017 1,055 4 View
Question for pathologist, cell biologist, Dengue research peoples
04 March 2017 3,684 4 View