Hi David,

I do not know if anyone here can assist you. It is quite a specialized technical question. A person who can definitely be of help is:

Vladan Rankov at The Advanced Technology Development Group, Gray Cancer Institute, Mount Vernon Hospital, Northwood, Middlesex, HA6 2JR, United Kingdom ([email protected]; phone +44 1923 828 611; fax +44 1923 835 210; www.gci.ac.uk)

Here is one of his papers:

http://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.96.6746&rep=rep1&type=pdf

He is working on image stitching issues in microscopy.

Let us know how it went :)

Hey Valeria,

In the end, the issue was the LED light had a brightspot in the center causing the problems. Looking at the objectives back pupil and turning shading correction off helped me come to the conclusion.

Thank you for the help.

David

Hi David,

We are all (I am sure) glad the issue got resolved favorably.