The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
My current protocol involves 2-methylbutane and dry ice. I do everything I can to ensure all reagents are as cold as possible so that freezing time is below 1 minute. I store 2-methylbutane at...
16 February 2018 8,340 5 View
I currently work with fluorescence microscope to observe generative and vegetative cells within hibiscus rosa sinensis pollen. I used DAPI to stain pollens but there is no light that indicate the...
07 February 2018 3,899 4 View
I need to determine ploidity levels in some wild species. How many cells should I look at to really be sure? I have been told that I need to see 30 roots from each plant and count in 30 cells from...
30 January 2018 6,247 3 View
Hi everyone! I was wondering if anyone has the experience of using super resolution microscopy for live-cell imaging. I found this review paper in this field. Super-resolution imaging in live...
19 January 2018 4,959 4 View
Hello! Recently I'm having more and more experiments with microscopy involved. Sadly I was barely educated in both theory and practice of optics and microscopy, and I was wondering, if there is a...
04 January 2018 2,398 3 View
Cyanobacterial identification based on microscopy
02 January 2018 6,468 2 View
I was doing a MTT assay on 2DG for my PC3 and LNCaP cell lines for my project, however I am not sure why MTT is used. (I mean due to time restriction of the project, I did MTT to save time but...
21 December 2017 8,851 3 View
I have a quite bright autofluorescence in the mouse brain in vivo. I can remove it only reducing the Gain or Laser Power, but it is appeared to be not enough to image my fluorescent dye....
30 November 2017 6,568 3 View
Hi there! Very interesting topic of research, this one. Any outputs available already? Thanks for your attention, Jorge Oliveira Pires Sao Paulo School of Economics Getulio Vargas Foundation, Brazil
29 November 2017 5,174 3 View
Is there any problems to use an air or oil-immersed objective? Thanks for all of your advice.
24 November 2017 7,342 2 View
I am interested in knowing the internal pH of coralline algae cells as well as the pH inside their cellular vacuoles. Has anyone measured the pH inside the vacuoles of a coralline alga? Either via...
14 November 2017 8,611 5 View
Hello all, I have several slides of 10um-thick cryosectioned brain slices from a line of FosTRAP (tdTomato fluorescent) mice that I am attempting to counterstain with DAPI Fluoromount-G prior to...
12 November 2017 1,459 4 View
I am fabricating carbon fiber blocks by Vacuum Assisted Resin Transfer Moulding(VARTM) to attain test piece by cutting the block with a water-jet cutter. Most study shows that they polish the...
07 November 2017 4,050 1 View
I look for fluorescent, water - soluble, stable label of lysozyme which will not cause denaturation of protein. I would like to dissolve lysozyme and fluorescent label in water and than see this...
29 October 2017 3,552 1 View
Hello everyone! I am a Ph.D. student and I am just starting my project. In this project we were considering the hypothesis of using miniaturized microscopes. I know that there are two companies...
28 October 2017 6,555 2 View
Which fixative will be useful for the fixing of this microalgae?A friend is advising i use Rose Bengal is it useful for this purpose and If so what will it be useful for?How do i prepare the Rose...
23 October 2017 6,892 3 View
I am studying a protein that is co—localized with The plasma membrane. I want to understand if it binds directly to it or via an adaptor. I want to use fluorescent microscopy and in vitro assays...
22 October 2017 2,962 2 View
I was viewing fecal sediments after FEACT from water buffaloes. One of the samples contained numerous "debris" that I'm unfamiliar with. I asked some colleagues if they were plant pollen or other...
20 October 2017 5,553 7 View
We are working on reflective DHM and the system looks like the setup in figure 1. We are facing a severe problem of reflection in the system. Apart from the reference beam and object beam, there...
14 October 2017 3,832 5 View
I am looking for a protocol to estimate the density of fungal biomass given an image from microscopy. A colleague described a method that involved point-intercept line sampling, but I have been...
05 October 2017 3,495 2 View
Does one add the high-concentration of KCL (~40-50mM) solution directly into the dish as its being imaged? Does the calcium-dye loading needed to be done in the above solution as well?
03 October 2017 8,568 2 View
We are interested in imaging the basement membrane (BM) dynamics; I am kindly asking if there are any fluorescent labels with high affinity to BM components namely, collagen or laminin that would...
01 October 2017 7,219 2 View
I'm currently looking at their diversity in forest using a microscope and I'm hoping to get references to compare my samples with. I'm having difficulty in researching especially the updated ones.
23 September 2017 7,102 6 View
If we have a cascaded microscope system made of :1. Microscope objective of numerical aperture NA12. Tube lens3. Microscope objective of numerical aperture NA24. Tube lens5. A recording...
22 September 2017 1,152 5 View