The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
I'm currently writing a software to analyse microscopy images. To give the user a better experience, i want to automatically extract as much information about the image as possible such as the...
03 April 2019 8,655 1 View
I am interested whether adding NADH to culture medium would lead to its uptake into the cytoplasm. I'm guessing it's not permeable to the cell wall/plasma membrane, but might there be active...
14 March 2019 1,750 3 View
By visualising in microscope
18 February 2019 5,609 0 View
In my efforts to characterise a 0.67 NA microscope objective, I have placed a 20 micron precision pinhole at the focal plane of the objective, and back illuminated the pinhole with a 767nm laser....
16 February 2019 221 0 View
I am not sure if Fluoromount G is a good media to perform TIRF because his refractive index. Has anyone tried?
25 January 2019 1,942 2 View
Hi, I am interested in testing the interaction of a plasma membrane receptor and the chromatin. I have tried several available protocols, but all of them showing contamination of the chromatin...
09 January 2019 5,996 0 View
What is the difference between bone marrow and peripheral blood with respect to fixation and immunofluorescent staining? We have a problem with non-specific staining for a range of antibodies on...
12 November 2018 9,472 0 View
Dear colleagues, I want to prepare a stock of 0.8% agarose gel for gel electrophoresis and my only possibility to store the (still liquid) stock woud be an incubator set at 37°C. Does anyone...
11 November 2018 4,683 4 View
what is the apomorphy that make difference between Glucophytes and green algaes?
28 October 2018 4,464 3 View
I am looking for a way to visualize plasma membrane in mouse airway secretory cells, in paraffin sections by immunofluorescence. In other words, it has to be a sufficiently abundant in plasma...
04 October 2018 3,615 9 View
There is a protein has two isoforms, one of which has a signal peptide. I am wondering if it is a secretory protein. If it is, how can I detect it? What if it is secreted by extracellular...
20 September 2018 1,551 2 View
During the charging phase of a lithium ion cell I found some small voltage drops (as shown in the attached figure). Can you tell me what they're due to?
03 September 2018 4,495 2 View
I have just learned photoacoustic imaging for less than one week. I am confused about some issues of optic-resolution photoacoustic microscopy (OR-PAM) and acoustic-resolution photoacoustic...
03 September 2018 4,542 3 View
I want to couple NP to PMSs so that it can be recognized by NP-spec. B cells.
21 August 2018 2,124 2 View
I am trying to setup an automated pipeline to acquire images of individual neurites in a sample. For this I am using the NIS elements software. I have both DAPI and Phalloidin available as...
08 August 2018 5,232 3 View
Greetings dear scientific community, Today I would like to ask a question which is partly related to my work. I have been doing microscopy as part of my job for four years now, and I always had...
02 August 2018 9,241 4 View
Just want to know if red blood cells could be detectate in far red (650-750nm wavelenght) under 514nm laser excitation.
27 July 2018 1,097 3 View
I am working on Symbiodinium cells (symbiotic dinoflagellates) and am trying to calculate the mitotic index. All the papers I have referred to use DAPI. I want to know if there are other stains or...
15 July 2018 232 3 View
While recording high speed images of MCF7 cells flowing in a microfluidic device, I've observed that some cells appear much brighter than the rest of the population. I've read that cells, just...
28 June 2018 2,046 2 View
Hi there, I have a stock solution of a clinical isolated phage with a titre of ~3E12 pfu/ml. Is it high enough for TEM microscopy, and is there any other considerations for imaging? Regards Alireza
24 June 2018 9,347 2 View
I am trying to distinguish the pillar cells from thin and thick epithelial cells of Chinese mitten crab so that I can study using pH dyes regulatory capabilities of the individual cell types....
14 June 2018 631 2 View
I have taken a Z-stack of a cell using a Zeiss confocal microscope. I have orientated the image in 3D and need to include a scale bar/take measurements in this orientation. Does anyone know how to...
27 April 2018 5,228 3 View
I have more than 10k images (Phase contrast, 10X) of hMSCs and I need to count the cells. Right now, I am using ImagaeJ jo manually count them in a 'tick-tick' fashion which is very laborious and...
25 April 2018 4,349 5 View
under microscopy how differentiate between the THP1 and Macrophages cells?
06 April 2018 6,231 3 View