The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
1,825 views 543 posts
Questions related to Microscopy
Hello. I am looking for something to stain brain cryosection tissue that is visible in normal light microscopy, but does not yield a significant signal with fluorescence microscopy (in range 405...
25 April 2017 553 3 View
Hello all, I have stained some bacteria using Gram staining process i.e. Gram Positive are stained with crystal violet and Gram negative are stained with sefranin. My aim is to see bacteria at...
24 April 2017 6,852 4 View
I usually use PFA 4%. However I must fix cells (Hs 578Bst) with a not hazard-toxic compound. I thought to use air flow comes of biological cape, but the morphology must be affected. Some...
20 April 2017 411 4 View
I'm trying to get better pictures of yeast mitochondria and the facility I have access to has a choice of spinning disk, Olympus FV1000 MPE, Olympus FV1000 - two photon, and Leica SP8 MP. I think...
04 April 2017 7,669 2 View
Magnet objects is very danger for electron microscope. Is is possible to get SEM images ? Thanks
03 April 2017 3,683 6 View
Greetings, here is friday morning! Nothing but, there is a question as to the microarray. I use Arrayit micro spotting solution 2X plus. As you can see in the photograph, after baking recommended...
31 March 2017 6,730 7 View
What can I use to test the performance of a Zeiss 880 Airyscan with PALM super-resolution? It is supposed to have 140nm Airyscan resolution and 20nm super-resolution. I'm looking for something I...
14 March 2017 943 4 View
03 March 2017 1,633 4 View
I want to preserve the morphological features of the bacteria for AFM analysis and I need a fast method for the fixation! Alcohol make some detachments in the bacterial proteins!! And...
03 March 2017 6,416 4 View
I would like to know the best way and substance(s) to make permanent slides of polychaetes' chaetae to microscopy examination based on your own experience.
23 February 2017 6,136 11 View
Dear all, I am new to TEM that I would like to know if there's any dendritic cell specific organelle under TEM observation? Birbeck body/ granule seems to be specific only for skin...
06 February 2017 1,013 1 View
02 February 2017 1,351 11 View
02 February 2017 5,943 1 View
Any idea how to promote contrast in a Cellulose / Chitosan sample for analysis in scanning electron microscopy?
02 February 2017 3,192 8 View
Samples are highly transparent silicale glasses. I'm trying to get images by reflected light microscopy from surface and cross-section.
02 February 2017 8,747 3 View
Hello! I'd like to capture some digital photographs of co-infiltrated tobacco leaves expressing a GFP reporter. Our lab uses a Nikon Coolpix P80 camera. If you have suggestions on a way to mount a...
31 January 2017 1,644 3 View
I have an old Olympus IX70 inverted fluorescence microscope and need to get a copy of the manual for its fluorescent light source (it is a separate manual from the microscope manual). I had a copy...
26 January 2017 3,607 2 View
We suspect from the contamination so is the pictures (Orange arrow) contamination or something else?
25 January 2017 5,746 4 View
Microscopy imaging (TEM and FESEM) showed collapse of mesopores while going from A to B. Beyond this I don't have much information related to structures.
11 January 2017 2,284 3 View
01 January 2017 7,429 3 View
For some reasons, I loaded the hand drawing pictures according to the TEM (Figure 1 and 2). The bacteria in the glycocalyx layer were also sometimes observed to be surrounded by filaments (the...
01 January 2017 4,643 5 View
Hi, Could you please share your experience regarding upsides and downsides of these microscopes? Which micrsocope is better for fluorescent imaging in fixed cells at 100x?
01 January 2017 7,460 1 View
01 January 2017 2,237 3 View
01 January 2017 8,118 2 View