The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
I have a stock solution of DAPI (5 mg/mL) dissolved in dH2O. To stain my fixed cells I dilute it with PBS to 300 nM as suggested by the manufacturer. However, after I diluted DAPI I observed small...
19 July 2023 9,383 2 View
I am studying niche overlap analysis and have obtained some results. My results show that species 1 and species 2 have a low Schoener's D value (approximately 0.1-0.2) and a high P-value from the...
04 July 2023 4,347 0 View
Currently, I'm searching the best method to quantify Jasmonic Acid in plant tissue. Previously I have tried to analyze by using GC-MS but I failed. I am planning to try HPLC and the available HPLC...
27 June 2023 6,785 3 View
please tell me If there is a standard ratio in rearing Tilapia fish in an aquarium.
13 June 2023 6,355 3 View
I have been trying to optimize this new antibody I bought recently. The band size is 146 kDa. I have loaded 30 microgram of protein used a dilution of 1:500 of primary antibody. But I keep getting...
24 May 2023 4,626 3 View
Hi everyone, I’m using RNAeasy plant mini kit of Qiagen in Arabidopsis. My concentration is good and also 260/289 value, but I’m having troubles with 260/230 in some of my samples I have 0,78...
20 May 2023 5,750 2 View
I am trying to count the chromosomes of Polypodium vulgare using a microscope (10*100X, Olympus BX51T), but I am facing some issues. I am not sure if all cells are in interphase or if some are...
01 May 2023 134 2 View
If we understand plant fragrance energy then it will help us to understand why plant gives more fruit, flower,leaf fragrance or less fruit, flower leaf fragrance and what it is problem: nutrient...
17 April 2023 6,726 0 View
Hello Everyone, please Can I get possible research topics for solar cells and separation membranes?
09 February 2023 456 2 View
Hello We implemented a small-sized VRLA (AGM Valve Regulated Lead Acid) battery project on the number of 10 ethers. In these batteries, sulfuric acid with a density of 1.250 and each battery cell...
19 January 2023 4,444 0 View
This diatom was collected from the half-salt water in Macau. This is a SEM figure.
12 January 2023 1,124 0 View
this cell is iPSC derived mesoderm. I dont know what makes my cells diying... please tell me What kind of phenomenon does it look like during the process of dying? I'd like to get a clue as to why.
27 December 2022 3,643 1 View
I want to know how to check the health status of the cell lines under the microscope
12 November 2022 2,541 2 View
I am generating fibroblasts from healthy donors and used them at early passages. Some of my colleagues lost their cultures after serum starvation prior the start of an experiment. Can overnight...
11 November 2022 4,412 1 View
Dear forum users, I am trying to automate the generation of ROIs on a fluorescence time lapse image of SH-SY5Y cells to quantify potassium flux. We aim to calculate baseline response,...
07 October 2022 6,564 1 View
I should stimulate a neutrophils culture to undergo apoptosis as a positive control for Annessin V kit. Which is the best way to induce apoptosis in a short time? Thanks
21 September 2022 3,083 1 View
I'm working on the antimicrobial activity of microbial metabolite on pathogenic bacterial strains. Can anyone tell me any alternative to SYTO9 fluorescent dye to observe live strain in fluorescent...
22 June 2022 3,456 2 View
Can a microscope equipped with a spinning disk Scan a Sample by the use of a reflection modality?
01 April 2022 2,052 0 View
Hey everyone! Have anyone faced an issue of how to minimize/quench autofluorescence in mammalian live cells? To be more specific I always stained and visualized ovarian cancer cell lines in blue,...
18 February 2022 2,433 0 View
I have been doing a subcellular fraction of Nucleus, Mitochondria, and ER in the HEK293 cell. But in results, I usually see all the three fractions have an equal amount of protein. Please help me...
20 December 2021 4,596 2 View
Hi all, Does anybody know how to segment the cytoplasm of cells using imageJ but excluding the nucleus (creating a donut-type selection). I would like to use this to create an region of interest...
04 August 2021 876 3 View
If possible please let us know about the cell type and the flurophore/dye used. Thanks. https://www.thermofisher.com/order/catalog/product/A50299?SID=srch-hj-A50299#/A50299?SID=srch-hj-A50299
23 June 2021 8,262 1 View
Hey, I have done the Transmission Electron Microscopy of my phages and got nice images. Now i've classified my phages depending on their morphology. In many papers, i have seen they have measured...
14 March 2021 5,727 1 View
Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...
26 February 2021 8,009 6 View