The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
Hello, all I am currently extracting LPS from E. coli MG1655 and its mutant strains. I followed several different protocols (phenol methods or phenol+diethy ether methods), but I couldn't see...
26 June 2024 7,444 1 View
Berry curvature is defined as \nabla_{R) \times A, where A is the Berry connection. This formula involves partial differentiations of the eigenvectors, often leading to problematic situations when...">
07 June 2024 6,142 0 View
We are slicing our human hippocampus with cryostat and wanted to know how much RNA (in ug) can you obtain from a cryostat slice? Obviously it depends on the thickness, but if anyone has any...
22 January 2024 584 2 View
If cells were transfected with a plasmid containing a gene of interesed (the resulting protein is intracellular) fused to GFP, would the GFP signal be detected by flow cytometry? Or can we only...
20 January 2024 754 1 View
My lab ordered a vial of the DAUDI cell line, but we are facing issues as the cells are growing very slowly, even after 4 months. Additionally, most of them are dying after only 4 passages,...
17 January 2024 8,049 3 View
I am working on CaSO4 processing project, where CaSO4 dihydrate is processed to hemihydrate. Can anyone suggest a proved procedure tom obtain CaSO4 hemihrdate that when formed beads can harden in...
15 December 2023 2,270 0 View
Does anyone know the org db for Solanum tuberosum or any such file that can be imported into R?
14 December 2023 2,644 0 View
The case is semiconductor electrodes immersed in 1M NaOH. The fermi level of the SC-electrode was more positive than O2/H2O electrochemical potential in some samples and less positive in other...
24 November 2023 608 0 View
This device would help to detect enemy or their `store of arms in tonels.
25 October 2023 3,531 0 View
Good day! I am trying to find a way to measure hesperidin content of the peels of my fruit sample (Citrus reticulata), other than HPLC. I plan to ferment my peels with food-derived mixed cultures...
14 October 2023 1,126 2 View
ِDear all, We work on breads made from frozen dough using sourdough. How can the growth conditions of lactobacillus be provided in cold temperatures?
11 October 2023 2,374 0 View
Dear community, I’m trying to lyse Lysobacter enzymogenes cells. I want to open them up in a very gentle way to cause as little damage to everything that’s inside them (proteins, peptides,...
09 October 2023 1,923 0 View
Hi there, I am working on a chorioallantoic membrane analiysis in thyroid cancer. Which microscopes can I choose to view chorioallantoic membrane analysis? Is there anyone who can help?
28 August 2023 8,056 1 View
What happens to relative humidity when dry bulb temperature increases and what happens to the relative humidity if the difference between dry bulb and wet-bulb is high?
25 August 2023 7,431 0 View
Could you please have anyone suggest to me what is the fastest and most efficient method to estimate the Fe, Zn, phytate, and protein content of wheat grain?
24 August 2023 1,954 1 View
I have a stock solution of DAPI (5 mg/mL) dissolved in dH2O. To stain my fixed cells I dilute it with PBS to 300 nM as suggested by the manufacturer. However, after I diluted DAPI I observed small...
19 July 2023 9,405 2 View
I ma doing a 25ul reaction for my qPCR. I know that making bubbles would destroy my results as it makes DNA polymerase does not work. I use centrifuge after I am done with pipetting but it is not...
11 July 2023 8,864 2 View
I am studying niche overlap analysis and have obtained some results. My results show that species 1 and species 2 have a low Schoener's D value (approximately 0.1-0.2) and a high P-value from the...
04 July 2023 4,367 0 View
Currently, I'm searching the best method to quantify Jasmonic Acid in plant tissue. Previously I have tried to analyze by using GC-MS but I failed. I am planning to try HPLC and the available HPLC...
27 June 2023 6,801 3 View
please tell me If there is a standard ratio in rearing Tilapia fish in an aquarium.
13 June 2023 6,376 3 View
I have been trying to optimize this new antibody I bought recently. The band size is 146 kDa. I have loaded 30 microgram of protein used a dilution of 1:500 of primary antibody. But I keep getting...
24 May 2023 4,647 3 View
Hi everyone, I’m using RNAeasy plant mini kit of Qiagen in Arabidopsis. My concentration is good and also 260/289 value, but I’m having troubles with 260/230 in some of my samples I have 0,78...
20 May 2023 5,770 2 View
I am trying to count the chromosomes of Polypodium vulgare using a microscope (10*100X, Olympus BX51T), but I am facing some issues. I am not sure if all cells are in interphase or if some are...
01 May 2023 157 2 View
If we understand plant fragrance energy then it will help us to understand why plant gives more fruit, flower,leaf fragrance or less fruit, flower leaf fragrance and what it is problem: nutrient...
17 April 2023 6,748 0 View