What is the difference between Hex Dominant and Cut Cell Meshing methods in CFD?

Though both the methods look similar I believe there may be differences in them. What are the disadvantages of cut-cell meshing method and why is not widely used in research in comparison with...

12 July 2020 5,636 3 View

Is SIMSCALE software a good to go option for visualizing and understanding academic concepts?

On what scale can SIMSCALE simulation software be helpful for students in their engineering course work comprising CFD ( Computational fluid dynamics ), thermal and structural concepts? Can...

29 March 2020 2,952 3 View

What is the correct procedure to balance the e viscous radiator fan. should we balance it with full assembly like fan, drive with visous oil & clutch?

1. How is / should be the balancing of Viscous Fan carried ? Is balancing of Fan blade & Drive separately to meet final assembly specification a correct method of balancing ? or it should be...

10 November 2019 1,782 1 View

How is the processive enzyme which ratchets DNA through a (oxford) nanopore attached the "right way about" to the nanopore?

I am curious about how the ratcheting of a DNA strand through a nanopore works in (oxford) nanopore sequencing. I think that the enzyme motor is first attached to one end of the DNA strand to be...

30 August 2018 7,786 1 View

What are the purification techniques available for water purification other than UV and RO system?

I need low cost water purification technique with readily available material for designing solar water heater cum purifier for rural households.

22 December 2017 864 2 View

Problem with DNA standard optamization?

I am preparing DNA standard curve using gag cloned plasmid by SYBR green assay. I have optimized my primers concentration (250nM) and prepared different DNA concentration based on the copy number....

29 August 2017 1,553 2 View

In scan rate study (sensor), why the diffusion controlled process shows negative intercept and adsorption controlled process shows positive intercept?

Because, I am getting negative intercept for my dopamine sensor (diffusion controlled process). When i am plotting the peak current vs square root of scan rate, i am getting negative intercept....

16 April 2017 2,200 3 View

Is there any way to find if my splice mutation has caused an alternative splice site .?

Although i have the cDNA sequence by RT- PCR..how do i still prove the alternative splicing

10 March 2017 8,262 3 View

Weather 3 wheels or 4 wheels are most suitable for hybrid electric vehicles?

what are the flexibility/importance of wheel selection in hybrid electric vehicle.

25 January 2017 4,768 2 View

Survival Analysis, Time to Event Analysis, SAS?

Hi There,I have a cancer dataset with 170 observations, I calculated the length of progression(in days) as the difference between date of surgery and date of cancer progression. Now that the date...

10 October 2016 623 11 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View