The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
I'm struggling to sort out an issue that all the sudden happened with a cleanliness inspector microscope.See the image attached. I've attempted the shading calibrations multiple times and it...
16 August 2019 1,609 3 View
Hello everyone, I'm trying to image neuronal tracts of specific neurons in the basal ganglia of an adult mouse, using viral retrograde tracers and light-sheet microscopy. Which method is...
09 August 2019 495 1 View
I'm relatively new to microscopy and staining. I am working with a mouseline that is susceptible to developing early onset alzheimer. I see that often times papers reference staining myelin...
28 July 2019 7,198 2 View
Hi! Have anyone ever tried single protein tracking (let's say a secretory protein) but also see its relative position to other organelles such as ER and nucleus (labelled by other...
22 July 2019 4,178 3 View
An apparatus was constructed in the lab to measure charge carrier lifetime. - An LED illuminates an n-typed germanium semi-conductor with a square function with a constant frequency (the period...
17 July 2019 1,760 5 View
Hi there, I am trying to use ImageJ to automatically quantify filopodial density and length on HT1080 cell surfaces in scanning electron microscopy images. I have noticed that most of the...
11 July 2019 9,443 2 View
I'm looking for a good protocol for taking fungi (mostly conidial ascomycetes) growing on an agar plate and fixing them and ultimately staining and mounting them. Would one simply remove a plug of...
04 June 2019 8,305 5 View
Hey, I'm looking for assays where I can track single cell - single bacterium dynamics, mainly rate of killing. Most methods I find online revolve around finding a single infected cell and...
29 May 2019 1,960 2 View
I’m facing a big problem with microscopy and would like to hear your suggestions. My aim is to visualize gamma-H2A.X foci in hameatopoietic stem cells under the microscope. When I try this or any...
28 May 2019 1,279 3 View
I have acquired some chep LED lights which apparently emith UV to NIR light (will need to measure it with a pyranometer in my setting anyway). I am trying to get a photocatalytic intrinsic...
21 May 2019 1,788 5 View
I need to collect blood samples for microscopy of P. falciparum. Which tube is best for this? Heparin or plain vacutainer tubes? Will RBC lysis affect detection of the parasite? Thank you.
24 April 2019 8,872 4 View
I'm currently writing a software to analyse microscopy images. To give the user a better experience, i want to automatically extract as much information about the image as possible such as the...
03 April 2019 8,545 1 View
I am looking for a way to visualize plasma membrane in mouse airway secretory cells, in paraffin sections by immunofluorescence. In other words, it has to be a sufficiently abundant in plasma...
04 October 2018 3,529 9 View
During the charging phase of a lithium ion cell I found some small voltage drops (as shown in the attached figure). Can you tell me what they're due to?
03 September 2018 4,390 2 View
I am trying to setup an automated pipeline to acquire images of individual neurites in a sample. For this I am using the NIS elements software. I have both DAPI and Phalloidin available as...
08 August 2018 5,166 3 View
Greetings dear scientific community, Today I would like to ask a question which is partly related to my work. I have been doing microscopy as part of my job for four years now, and I always had...
02 August 2018 9,157 4 View
Just want to know if red blood cells could be detectate in far red (650-750nm wavelenght) under 514nm laser excitation.
27 July 2018 1,034 3 View
I am working on Symbiodinium cells (symbiotic dinoflagellates) and am trying to calculate the mitotic index. All the papers I have referred to use DAPI. I want to know if there are other stains or...
15 July 2018 137 3 View
While recording high speed images of MCF7 cells flowing in a microfluidic device, I've observed that some cells appear much brighter than the rest of the population. I've read that cells, just...
28 June 2018 1,940 2 View
Hi there, I have a stock solution of a clinical isolated phage with a titre of ~3E12 pfu/ml. Is it high enough for TEM microscopy, and is there any other considerations for imaging? Regards Alireza
24 June 2018 9,246 2 View
I am trying to distinguish the pillar cells from thin and thick epithelial cells of Chinese mitten crab so that I can study using pH dyes regulatory capabilities of the individual cell types....
14 June 2018 545 2 View
I have taken a Z-stack of a cell using a Zeiss confocal microscope. I have orientated the image in 3D and need to include a scale bar/take measurements in this orientation. Does anyone know how to...
27 April 2018 5,159 3 View
I have more than 10k images (Phase contrast, 10X) of hMSCs and I need to count the cells. Right now, I am using ImagaeJ jo manually count them in a 'tick-tick' fashion which is very laborious and...
25 April 2018 4,272 5 View
under microscopy how differentiate between the THP1 and Macrophages cells?
06 April 2018 6,149 3 View