The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
2,231 views 639 posts
Questions related to Microscopy
I'm tracking endosomes inside the cell to graphically show an anterograte (towards the membrane) or retrograde (to the perinuclear zone) traffic. What I am doing is tracking edosomes along their...
28 June 2020 1,453 0 View
I have been using conjugated antibodies for staining cancer cells. And I try preserving the fluorescence signals using Prolong antifade with Dapi. However, after 24-48 hours, the only signal I see...
10 June 2020 4,825 1 View
Please I need some technical advise on multiplex IF for at least 3 proteins: I am trying to image 3 different endogenous proteins by IF-confocal microscopy, but only have primary antibodies raised...
11 May 2020 2,261 1 View
Darkfield and fluorescence microscopy
16 April 2020 9,205 1 View
Fluorescent staining and further microscopy is a powerful tool in studying microscopic strictures. Are there any other dye systems available that can stain bacterial cells as SYTO9 does?
09 April 2020 9,220 7 View
Hi I would like to know if is it possible to obtain the crystallinity of a material such as starch from the ratio of the birefringent area to total area using the images obtained from light and...
31 March 2020 1,245 5 View
I need to compare the sample for porosity determination by using x-ray CT and optical microscopy. All views and suggestions are welcome.
28 March 2020 6,561 3 View
I am a microscopy image analysis noobie.It'd be great to have a discussion on this question.We have multi channel fluorescence images of dendrites and other brain structures obtained before and...
27 March 2020 9,875 3 View
Hi everybody, Does anybody have experiences like getting different lifetime result by changing magnification from a sample? Can this factor lonely has influence on the lifetime of a tissue? Bests
21 March 2020 8,883 3 View
I have a grayscale image of which the original RGB image was formed by combining Green and Red channels which represent different types of cells (live/dead). Splitting the channels in RGB mode is...
12 March 2020 1,884 3 View
For capsule staining I used over night bacteria culture and smear with 1% congo red . After that, dry the slide in room temperature and observe under light microscopy 100x magnification oil...
18 February 2020 7,509 8 View
I have two images, taken in the same camera, one taken with a 10X objective and another with a 5X objective. The pixel/micron ratio for the 10X image is 1px=1.25um. But, I do not have a...
17 February 2020 9,756 2 View
I used India Ink and 1% CV for Identification CPS layer in oil immersion Light microscopy (100x). My bacteria sample is Acinetobacter baumannii .
02 February 2020 9,579 3 View
Dear all, I am preparing wood charcoal for systematic vessel measurements, using an SEM and software that automatically recognises pores. I however have problems to achieve straight transversal...
27 January 2020 2,493 3 View
Hi all! Is there any reference or suggestion which can help us to set the sampling protocols for the sample collection related to electron microscopy (SEM and TEM). As we are working in...
20 January 2020 5,896 2 View
Hello. I came across a fixation protocol for optical microscopy that employs chromium trioxide as a fixative. I was wondering if you guys could point me towards a less-toxic substitute for this...
09 January 2020 4,797 3 View
Viewing in microscope while changing fields I find bit confusing. Any software or any other method is there as I need to count total 2000 cells
30 December 2019 9,900 2 View
which one is the best microscopy among SEM. TEM. LEM .. for fruit peel cuticle wax thichness measurement? and how can make sure the cuticle wax layer ?,
27 December 2019 4,674 11 View
How about H202 concentration use to kill CD spore? Thanks in advance
12 December 2019 3,318 2 View
Hi, I just wondering is it possible and if yes, how to find the gel-point of an epoxy resin, using Dynamic Mechanical Analyzing (DMA). I have used other methods, and want to compare the results....
03 December 2019 1,201 5 View
I am looking for a PRIME BSI, a back-illuminated sCMOS camera, to be used in a Spinning Disk Confocal Microscope. This camera is much cheaper than an Andor camera. I would say that from the...
29 November 2019 8,811 0 View
I have these oxide/metal/oxide sandwich samples that the thickness of it reaches below 100 nm in the areas indicated in the picture. Those are oxide/oxide regions. I am just concerned about...
26 November 2019 5,239 4 View
I have different fungal colonies so now i would like to identify them, so Which microscope is suitable and easy to operate for identification and taking pictures of fungus?
15 November 2019 9,751 3 View
I am working with E. Coli biofilm samples. Our method use a paraformaldehyde fixation step in order to preserve biofilm structures before microscopy observation. However, we have saw that there...
25 October 2019 7,791 4 View