The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
I would like to test the interaction between 2 proteins using FRET-FLIM microscopy and the fluorescent proteins mCitrine as donor, mCherry as acceptor. Has anybody ever tried to use these...
19 September 2017 1,956 2 View
I have a polished section with crystals of various reflectivity and I want to differentiate between them. Does anyone know if there is a standardised method to measure reflectivity using optical...
19 September 2017 3,123 2 View
I am working with macro-molecules composed of phospholipids in the 1 to 5 micron range and am having difficulty finding a microscopy tool to examine them under. I know cryo-sem would work, but...
07 September 2017 794 4 View
CAFM were performed in tapping mode with a conductive (Pt/Ir) probe.
07 September 2017 2,100 2 View
I want to image cells without altering protein levels and going to do so by gene editing the proteins I am interested in. I was doubting between directly fusing the protein of interest with a...
01 September 2017 7,143 3 View
I have seen that the Petroff-Hausser chambers work but they are cost prohibitive for my lab. Are there other options?
31 August 2017 8,253 1 View
I'm trying to measure the average raft culture thickness from my 3D-organotypic cultures using ImageJ but haven't been very successful. Can anyone suggest a tool or method for this? I've uploaded...
25 August 2017 7,541 3 View
Hi all, did you ever used SYBR green for fluorescence microscopy application (perhaps in combination with PI) ? Any tipps, like concentration used, special cares to have, etc..?Thanks!
08 August 2017 8,397 2 View
anybody knows how I can get propidiom iodide stainining image by olympus BX53. I couldn't find defined channel for PI?
26 July 2017 7,445 1 View
Should clinical haematologists put their microscopes on E-Bay? (van't Veer M, Haferlach T. Haematologica, 2014). I think no. The ability in recognizing cellular morphology in peripheral blood...
15 July 2017 1,123 33 View
I am not familiar with these microscopes as I was working with a different brand/model of microscopes before. I'm currently using a cx41. However the HPO is quite blurred and the only other...
13 July 2017 7,708 1 View
Hi, I'm doing the transwell invasion assay using MDA-MB-231 cells. The problem is that ,when I take the image after staining the cells, I found that the cells is not distributed evenly in the...
27 June 2017 251 9 View
a) Leica DM4B b) Zeiss Axioimager 2 c) Nicon Eclipse NiE d) Olympus BX53 How config is the best performance for FISH staining of chromosome? Could you give me a detailed config of fluorescence...
10 June 2017 1,925 3 View
Immersion oil has gotten all over the body of our Nikon Eclipse Ts2R and it won't come off. Lens cleaner takes care of the objective lens, but the rest of the knobs/base/etc. is tacky, dirty, and...
06 June 2017 4,872 3 View
We want to conduct immunogold labelling for scanning electron microscopy. As this is our first experience, I would like to know how long the samples can be kept in the fixation solution after...
31 May 2017 8,298 3 View
I'm trying to measure the cross section of collagen fibers using Bruker's nanoscope analysis software. I know, I have to flatten the image and then need to use section or step to get an output....
29 May 2017 4,095 1 View
I often find these items (marked with red arrows) while doing equine FECs. I am interested to know what they are. I am pretty certain that they aren't parasite eggs, and I find them in samples...
24 May 2017 4,780 9 View
We are interested in adapting a Confocal microscope for long-term live imaging, and for this reason we are trying to get a small environmental chamber at a reasonable prize. It could be enough...
17 May 2017 5,520 2 View
Does anyone have experience dealing with 3D tracking of microscale system, especially those can be embedded into microscope? My working system is at microns, so I don't need super resolution. One...
08 May 2017 5,047 2 View
I am working on a dissection fixation protocol for a non model organism and I am using DM1A alpha tubulin as a control to see if immunos work with a given trial of protocol. I think I got to a...
07 May 2017 4,150 2 View
Hi, I am trying to source an LED module suitable for insertion in the side port (ca. 8 mm diameter) for coaxial illumination in my microscopy setup. I have searched the sites such as Thorlabs and...
26 April 2017 1,995 2 View
Any ideas on how to couple a laser source to a infinity corrected microscope would be appreciated
25 April 2017 796 2 View
Hello. I am looking for something to stain brain cryosection tissue that is visible in normal light microscopy, but does not yield a significant signal with fluorescence microscopy (in range 405...
25 April 2017 566 3 View
Hello all, I have stained some bacteria using Gram staining process i.e. Gram Positive are stained with crystal violet and Gram negative are stained with sefranin. My aim is to see bacteria at...
24 April 2017 6,870 4 View