The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by... | Contact experts in Microscopy to get answers
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Questions related to Microscopy
I am studying a protein that is co—localized with The plasma membrane. I want to understand if it binds directly to it or via an adaptor. I want to use fluorescent microscopy and in vitro assays...
22 October 2017 3,030 2 View
I was viewing fecal sediments after FEACT from water buffaloes. One of the samples contained numerous "debris" that I'm unfamiliar with. I asked some colleagues if they were plant pollen or other...
20 October 2017 5,615 7 View
We are working on reflective DHM and the system looks like the setup in figure 1. We are facing a severe problem of reflection in the system. Apart from the reference beam and object beam, there...
14 October 2017 3,901 5 View
I am looking for a protocol to estimate the density of fungal biomass given an image from microscopy. A colleague described a method that involved point-intercept line sampling, but I have been...
05 October 2017 3,573 2 View
Does one add the high-concentration of KCL (~40-50mM) solution directly into the dish as its being imaged? Does the calcium-dye loading needed to be done in the above solution as well?
03 October 2017 8,645 2 View
We are interested in imaging the basement membrane (BM) dynamics; I am kindly asking if there are any fluorescent labels with high affinity to BM components namely, collagen or laminin that would...
01 October 2017 7,294 2 View
I'm currently looking at their diversity in forest using a microscope and I'm hoping to get references to compare my samples with. I'm having difficulty in researching especially the updated ones.
23 September 2017 7,147 6 View
If we have a cascaded microscope system made of :1. Microscope objective of numerical aperture NA12. Tube lens3. Microscope objective of numerical aperture NA24. Tube lens5. A recording...
22 September 2017 1,212 5 View
I would like to test the interaction between 2 proteins using FRET-FLIM microscopy and the fluorescent proteins mCitrine as donor, mCherry as acceptor. Has anybody ever tried to use these...
19 September 2017 2,017 2 View
I have a polished section with crystals of various reflectivity and I want to differentiate between them. Does anyone know if there is a standardised method to measure reflectivity using optical...
19 September 2017 3,195 2 View
What are the ways (in cell or cell free systems) through which we can experimentally prove that a chemical compound/drug molecule leads to complex formation between two proteins? I did...
19 September 2017 6,024 11 View
I am working with macro-molecules composed of phospholipids in the 1 to 5 micron range and am having difficulty finding a microscopy tool to examine them under. I know cryo-sem would work, but...
07 September 2017 854 4 View
CAFM were performed in tapping mode with a conductive (Pt/Ir) probe.
07 September 2017 2,191 2 View
I want to image cells without altering protein levels and going to do so by gene editing the proteins I am interested in. I was doubting between directly fusing the protein of interest with a...
01 September 2017 7,222 3 View
I have seen that the Petroff-Hausser chambers work but they are cost prohibitive for my lab. Are there other options?
31 August 2017 8,353 1 View
I'm trying to measure the average raft culture thickness from my 3D-organotypic cultures using ImageJ but haven't been very successful. Can anyone suggest a tool or method for this? I've uploaded...
25 August 2017 7,636 3 View
Hi all, did you ever used SYBR green for fluorescence microscopy application (perhaps in combination with PI) ? Any tipps, like concentration used, special cares to have, etc..?Thanks!
08 August 2017 8,460 2 View
anybody knows how I can get propidiom iodide stainining image by olympus BX53. I couldn't find defined channel for PI?
26 July 2017 7,526 1 View
Cell death was rescued by 50 μM Z-VAD but I still have destabilization of the phosphorylated form of the oncoprotein (simply as clear as treated band) in Western blot. I have also tried with...
17 July 2017 9,632 5 View
Should clinical haematologists put their microscopes on E-Bay? (van't Veer M, Haferlach T. Haematologica, 2014). I think no. The ability in recognizing cellular morphology in peripheral blood...
15 July 2017 1,193 33 View
I am not familiar with these microscopes as I was working with a different brand/model of microscopes before. I'm currently using a cx41. However the HPO is quite blurred and the only other...
13 July 2017 7,781 1 View
Hi, I'm doing the transwell invasion assay using MDA-MB-231 cells. The problem is that ,when I take the image after staining the cells, I found that the cells is not distributed evenly in the...
27 June 2017 315 9 View
I was testing some extracts with this assay and all of them gave a % of viability higher than 100%. I´d like to know if there is anything I did wrong.
15 June 2017 7,608 3 View
a) Leica DM4B b) Zeiss Axioimager 2 c) Nicon Eclipse NiE d) Olympus BX53 How config is the best performance for FISH staining of chromosome? Could you give me a detailed config of fluorescence...
10 June 2017 2,031 3 View