Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
since dispersion correction GD3BJ is incompatible with minnesota, which dispersion correction should be added in calculation.
06 June 2024 7,996 1 View
Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...
05 June 2024 4,082 3 View
Hi. i have a molecule it has both amide and ester bond. When i want to get second amide bond with ester part of the molecule by an amine, but it attacks to the amide bond also. In which conditions...
02 June 2024 9,853 2 View
Below is a diagram of TEC zones on earth as a .jpeg file.
26 May 2024 4,041 0 View
Hello I am looking for sulfo-phospho vanillin assay protocol for lipid quantification, I kindly request to provide protocol, it would be great help. Thanks
21 May 2024 6,773 0 View
Hello everyone, I’m delving into the functionalities of the Cobas Z480 analyzer and I’m eager to learn not only its basic operating principles but also about the different real-time PCR tests,...
21 May 2024 7,852 0 View
In photoautotrophic cells under light conditions, what proportion of the total ATP is generated within the chloroplasts, as a rough estimate or within a lower and upper bound, and what proportion...
15 May 2024 6,482 2 View
I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...
13 May 2024 6,530 2 View
Hello everyone. I need to be able to take cell culture media that has already been on cells and essentially make three different solutions from it: lipids, nucleotides, and proteins. The eventual...
30 April 2024 2,806 0 View
Hello all, I am currently in the process of isolating stress granules from mammalian cells transfected with GFP-tagged FUS mutant. I could see the stress granules clearly before and after cell...
23 April 2024 2,522 2 View
Hello everyone, I have performed EMSA experiment with an RNA-binding protein and RNA. For both ssRNA and dsRNA, for higher concentration of protein, I observe a high intensity band at the dye...
19 April 2024 10,024 1 View
Hello everyone, I am planning to do a cloning experiment. Can anyone suggest what is the minimum amount of insert DNA and vector DNA required for a 1:7 ligation reaction ? The size of my insert...
18 April 2024 5,834 2 View
Dear all, I used the the software "Phototox Version 2.0" to calculate PIF and MPE. In a dose-effect figure generated by the software contain some data, see attachments for details. Does anyone...
18 April 2024 1,162 0 View
My study is about synthesizing fish scales into chitosan and I am currently washing my demineralized fish scales. My sample fish scales were size reduced beforehand and when 1% HCl was added, it...
17 April 2024 2,121 0 View
Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide? What is the protocol?
17 April 2024 1,403 1 View
Has anyone done extensive characterization of Innoprot immortalized corneal epithelial cells? Please cite the references if there are any!!! Thanks in advance !
17 April 2024 1,025 0 View
hi I am planning to use tissue carriers consisting of two rings (base and tension) that can trap tissue or membrane in between them so I can utilize both surfaces of membrane (image attached),...
16 April 2024 9,819 0 View
Hello, I am currently exploring different thymidine treatments on Hela Cells. However, my Fascan profiles consistently reveal two peaks, suggesting that none of the thymidine conditions are...
08 April 2024 8,054 1 View
Hello, I did a qPCR experiment comparing expression level of several genes between two groups, each contains samples from 4 animals, and got certain results. I then wanted to increase the sample...
08 April 2024 1,003 0 View
I have a problem with conventional PCR electrophoresis, we are analyzing the deletion of ccr5 delta 32. And this material started to cause problems recently, there was no change in the protocol...
04 April 2024 3,885 3 View
I am planning an immunoprecipitation experiment using Mouse monoclonal [11C9] to Mannan Binding Lectin/MBL (ab26277) antibody to immunoprecipitate for MBL2 in human serum. There are many protocols...
27 March 2024 3,499 4 View
Hello, everyone Do you know some techniques to improve the efficiency of reprogramming if I use a kit (cytotune-ips 2.0 sendai vector) expired in July 2021? Thank you for your answers!
26 March 2024 7,751 4 View
Hello everyone, I am looking for any dye that can be used to determine the flow of liquid in a tube. Therefore, it should not affect tissues and cells or bind to nucleic acids or proteins....
26 March 2024 8,654 2 View
I have confusion in required Temperature.
26 March 2024 3,186 1 View