Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I am looking for answers as to whether NaHCO3 buffered cell culture media stored outside a CO2 gassed atmosphere changes it's pH irreversibly. I understand how the NaHCO3 buffer principally...
04 January 2024 8,310 3 View
Exploring the potential of edge computing to bolster security measures in Internet of Things (IoT) networks.
04 January 2024 2,532 1 View
My article entitled "Exploration and collection of root and tuber crops in East Wollega and Ilu Ababora zones: Rescuing declining genetic resources" is highjacked by some profile in Research gate...
29 December 2023 7,758 3 View
To understand the relationship between change in marginal error and research sample size.
28 December 2023 8,635 3 View
Whether one independent variable in multilevel regression can be the other two independent variables, such as type, token, and type/token ratio
28 December 2023 8,783 4 View
Currently, I am measuring gamma-secretase activity by lysing mouse brain, obtaining extract, and processing substrate that reacts with gamma-secretase in mouse brain. The measurement is based on...
27 December 2023 6,257 0 View
How to update H-index.
27 December 2023 594 2 View
i am not able to update my citations and h index
27 December 2023 4,854 5 View
How do I add more publications to my ResearchGate account? Frank J. Hoffman
27 December 2023 1,303 2 View
After cabonization and activation, biomass cellulose were transformed to biochar. But when I collected the XRD specturm, a stranger peak at 10° appeared. how could explain this peak.
26 December 2023 5,553 2 View
I was having a discussion with a friend about the best way to resuspend a cell pellet, flicking the tube or pippeting up and down. I wanted to bring the discussion to this community and ask what...
22 December 2023 4,270 1 View
I have a set of experiments ran in triplicate and I have some data points that seems like an outliers in each one? Which method is the best way to examine that? i would appreciate recommendations
22 December 2023 6,734 2 View
[email protected] Dear Prof. Fariha Kanwal I hope this letter finds you well. It is my honor to extend to you an invitation to speak at our upcoming scholar's conference for researchers and...
17 December 2023 4,855 66 View
Hi ResearchGate community, can anyone help us out with this contamination? We work with iPSC-NPC. This contamination happens ONLY when we put the neuronal progenitors in coverslips + media for...
15 December 2023 6,397 0 View
It should be noted that the synthesized particle has a blue emission
15 December 2023 5,112 2 View
Thank you
15 December 2023 2,324 1 View
I have tried to find it online but couldn't.
14 December 2023 441 1 View
Hello! I am investigating the extrudability of our bio-ink through rheology. Previously, I used a parallel plate to acquire the shear rate of the sample after which I tried an actual...
13 December 2023 9,470 6 View
Dear ResearchGate community, I am currently a master's degree student at Iran University of Medical Sciences, Department of Biotechnology, embarking on my first-ever exploration of aging and...
12 December 2023 9,450 0 View
i use 0.05% EDTA-trypsin to detach Hep G2 cells 6min then neutralize the trypsin- EDTA with DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA) and centrifuge gently then resuspend cells in fresh...
11 December 2023 335 5 View
Hi, I will be culturing BM-MSCs in DMEM culture media, based on the literature, I will add 10% FBS and pen/strep... except when I checked the one that we have, I found that we only have...
11 December 2023 6,628 3 View
I was wondering if you know of any online tools for analyzing RNA-seq results? Thanks in advance!
08 December 2023 5,743 3 View
I am working through the challenges of cell isolation from the rat heart. I am using VSMC media and endothelial cell media. In a homogeneous mixture of cells, would these media favor only the...
07 December 2023 4,949 1 View
we are analyzing activity of an endolysin which has N-terminal domain involved in antibacterial activity. The recombinant protein is not showing activity, and we are wondering that whether His-tag...
07 December 2023 8,777 6 View