Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
I have gene expression data from skin cells before and after treatment, and I want to normalise the expression of these two samples against the expression of 3 housekeeper genes. How would I go...
05 October 2022 7,056 1 View
Hello everyone, After lentiviral transduction my I started the antibiotic selection to select the positive clones. The transfected cells did not die suggesting that the transduction worked while...
02 October 2022 1,032 1 View
Hello fellows, Currently I am working on karyotyping of infertile males. I have met a case with abnormal structure in chromosome 9. Does anyone have seen or known this kind of...
30 September 2022 916 0 View
My lab has been having issues re-amplifying a M13 phage library, which was donated to us from another lab. It yields binders that we have since validated, so there's no issue with the library...
13 September 2022 4,659 1 View
Sudden reversal in magnetic field is the origin of switchback. The reconnecting field lines creates shear driven turbulence.
10 September 2022 8,440 3 View
How much 0.2% Saponin should I add to lyse 1X 105 THP-1 derived macrophages per well in a 96 well TC plate? Most of the papers referred to do not mention a volume or ratio. Only one paper...
28 August 2022 609 0 View
I have planned to infect THP-1-derived macrophages with Mycobacterium tuberculosis. After infection, cells would be lysed to quantify intracellular viable bacteria. How should I prepare 0.2%...
26 August 2022 3,795 3 View
I want to modify the surface of an exosome with a targeting peptide and a cytokine (IFN-y). Is this possible to do and how would I go about doing this?
13 August 2022 8,322 0 View
Hi everyone, I started culturing primary human melanocytes and am facing some problems. They grow very slowly, and after 15 days there seems to be no more growth (50% confluence). I follow...
05 August 2022 8,832 0 View
I am working on a GPCR project. In theory, it should be a homodimer. however, I found the tetramer during cryo-EM data analysis. And there is no reports about its agonist and antagonist. Is there...
05 August 2022 208 1 View
Dear all, I am carrying out fermentations in bioreactors using the haloarchaea strain Haloferax Mediterranei for the production of PHA with short fatty acids as the carbon substrate in a saline...
28 July 2022 9,613 4 View
Hi there! I am trying to quantify total inorganic phosphate from plant samples using a spectroscopy based techniques. I am facing issue with the standards. I have not tested the samples yet. For...
22 July 2022 7,142 3 View
After attaining 80% confluency of SH-SY5Y cells in a T25 flask with characteristic neuronal morphology, I seed into 96-well plates, and the cells are still rounded and appear shrunken after 24...
20 July 2022 3,157 4 View
was reading an older textbook (Duddeck) they describe H,C correlated COSY, interpreted as C connected to H. Is this essentially interpreted the same as HSQC and HETCOR?
11 July 2022 8,119 3 View
Good evening, I have started my culture with HUVEC cells and HAoEC, I use Endothelial Cell Medium (Cat1001) from sciencell, so the complete medium contains FBS, antibiotics and endothelial cell...
10 July 2022 5,823 1 View
I noticed that researchers use MOG35-55 to induce a type of T cell-dependent EAE, while for both T cell and B cell-dependent EAE, they choose rhMOG. Some papers report that B cells also contribute...
06 July 2022 4,337 1 View
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05 July 2022 7,843 3 View
Dear colleagues, I need to isolate Neutrophils from human blood using percoll. If you have experiences with this, could you please sharing your protocol? Besides, neutrophils of course could...
30 June 2022 2,453 3 View
Which bacteria serve as positive and negative control group for Protease, amylase and lipase enzymes?
28 June 2022 7,644 2 View
I did an LDH assay and have six concentrations of drugs I treated my cells with and when I put the LDH assay data in a graph, I see a normal distribution pattern. The 3rd concentration of the drug...
23 June 2022 6,528 2 View
I am working on natural and synthetic insulin mimetics and insulin potentiating agents. I am assessing the effect compounds on glucose uptake in L6 myotubes, for that I want use Insulin as...
23 June 2022 1,167 2 View
Hi, No one in my lab has experience growing U937 and I would like to know What's the best way to subculture the cells ( using RPMI-1650, 10% FBS and 1%PS), Do they attached or maintained in...
18 June 2022 6,763 1 View
I would like to identify nuclear lipid droplets with immunofluorescence in retina cyosections. I know that lipid droplets express several proteins. But the ideal would be a protein that is express...
17 June 2022 5,847 0 View
Ethanol prices have tripled since 2020 making the use of 70% ethanol non viable. I am looking to buy bulk quantity of disinfectant for our new laminar flowhood. I am wondering which type of...
17 June 2022 6,092 4 View