Cell culture is the complex process by which cells are grown under controlled conditions, generally outside of their natural environment. | Contact experts in Cell Culture to get answers
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Questions related to Cell Culture
Power generation process from nuclear and fossil fuel fired thermal sources heats environment with their rejected waste heat that constitute about 60% of the causes of global heating. But our...
3 weeks ago 1,564 1 View
I am conducting Western blot analysis using the Phospho-IKB alpha (Ser32) antibody (YA183) on an HSF cell line. However, I am observing fuzzy bands across the entire membrane. What could be the...
3 weeks ago 388 1 View
I did compression testing and my triplicates do not have the same strain step (varies in x), i am planing to interpolate the data to uniform the data. what could be a suitable method?
3 weeks ago 2,154 0 View
Hello everyone, I'm having persistent issues with a colorimetric hydroxyproline assay on decellularized tissue samples (ECM). I'm following protocols based on chloramine-T(oxidation buffer ph is 6...
4 weeks ago 1,218 0 View
I am currently exploring the feasibility of using non-invasive capacitive sensing to detect carotid artery stenosis. The idea is to measure changes in electrical capacitance caused by blood volume...
4 weeks ago 5,530 1 View
I’m currently trying to differentiate Th17 cells from purified CD4+ T cells isolated from human PBMCs. My current protocol involves: Activation: 1 µg/mL anti-CD3 and 1 µg/mL anti-CD28 Polarizing...
last month 6,786 3 View
Hello, I am currently culturing U87 MG cell line. Pic 1) This photo was taken 48 hours after seeding, using medium with 10% FBS. Pic 2) This photo was taken 72 hours after seeding. At this...
last month 2,489 1 View
My lab is not "rich" but is longing for high-throughput western blotting. I came across the SimpleWestern (Bio-Techne), but am not sure if this is worth investing. Is there information out there...
last month 6,388 1 View
Hi all, Hope everything good. I came across a problem. My NEB enzyme xho1 and Nhe1HF did not work. I check them one by one using PCAGGS, and it looks good. The xho1 have a little worse...
last month 4,872 3 View
How is the excitation defined in CST when the antenna is bent Did you use waveguide ports, discrete ports, or lumped ports? Why did you choose this type of excitation?
05 August 2025 425 2 View
I make sure the plates are cleaned with both ethanol and AMQ before starting, and I make sure to dry them too. APS is freshly prepared each time and I pace the pout=ring so that it is not too slow...
01 August 2025 2,912 0 View
Hello everyone, I’m working on recording the ¹H NMR spectrum of a folic acid–chitosan conjugate. When we attempted to dissolve the sample in D₂O, it did not dissolve properly. The operator added a...
29 July 2025 8,412 2 View
I have been trying to grow the Medulloblastoma cell line D341 for weeks now but they are extremely slow growing! I used the exact media and supplementation suggested by the ATCC. These cells grow...
21 July 2025 719 1 View
Hi, I noticed something weird during agarose gel electrophoresis. I use SYBR Green to stain the DNA. When my DNA sample shows faint bands, the ladder also looks faint. But as far as I know the...
13 July 2025 7,996 3 View
Any experiences any of you may have had with this problem, and your willingness to share treatments and outcomes would be greatly and gratefully appreciated. Thank you.
09 July 2025 9,165 0 View
I have a general question regarding the isolation of extracellular vesicles (EVs). I have read in various papers that EVs isolated from cell culture media are initially grown in media supplemented...
08 July 2025 3,387 2 View
Is Formamide (HY-Y0842, MCE) Toxic? What precautions should be taken during handling?
08 July 2025 5,886 3 View
Why did I get different restriction digestion results from three plasmid preparations, even though they all came from the same single colony of BL21(DE3) carrying the pET-SUMO-Lcp3 plasmid? I...
05 July 2025 399 9 View
I insert a sequence into the plasmid at the PspLI and BamHI restriction sites. The sticky ends of these restriction enzymes share two of four identical nucleotides. This likely explains why I get...
28 June 2025 634 13 View
I'm working on assembling a ~7.6 kb plasmid using restriction digestion followed by T4 DNA ligation. After ligation, I ran the product on a 0.7% agarose gel at 80V to check the assembly....
25 June 2025 1,876 5 View
I would like to ask about a confusion in siRNA experiments (siRNA set bought from MCE): In recent experiments, the target gene mRNA level dropped significantly, but the protein expression did not...
21 June 2025 4,266 1 View
I am looking for a detailed protocol for western blot using DAB method.
16 June 2025 2,328 3 View
After inducing cell lipid droplets (such as treating liver cells with oleic acid), I tried to dissolve the lipid droplets by lipid extraction or drug treatment, but found that the effect was not...
13 June 2025 8,664 1 View
I am planning to design an in vivo dosing scheme for Paclitaxel (HY-B0015, MCE). Your technical support recommends using a mixed solvent of 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline to...
12 June 2025 3,097 1 View