How can I make sure a protein with a signal-peptide is secreted or not?

15 April 2019 0 3K Report

There is a protein has two isoforms, one of which has a signal peptide. I am wondering if it is a secretory protein. If it is, how can I detect it? What if it is secreted by extracellular vesicles pathway?

Thank you for your help!

Badges
Science method

More Garshasb Rigi's questions See All

What is the best choice of spiral curriculum implementation approach?

Hello Dears Is the implementation of spiral curriculum at the clinical field valuable concept-based or problem-based or value-based or experience-based or evidence-based?

15 December 2023 2,959 2 View

Anti-Cancer Drug?

Is there any anti-cancer agent that no effect on cell survival but do effect other thing to suppress cancer proliferation/migration/invasion?

03 April 2019 6,767 3 View

Has anyone freezed samples at -80 without using any RNA later for RNA extraction?

There is a question in my mind that bothers me,I am collecting three kinds of samples :blood,saliva and tissue,I have stored all of my samples at -80 immediately after collection,I have put tissue...

24 January 2015 4,680 14 View

What are the safety issues to consider in industrial recombinant protein production using E. coli?

I'm studying about recombinant protein production now. When producing recombinant protein in large scale using E. coli, there are several problems including endotoxin and bad smell etc. I want to...

01 January 1970 4,162 3 View

How to remove endotoxin-LPS in protein?

Hi, I have one question. When express one protein (animal protein drug ) in E.coli system, E.coli endotoxin such as LPS will reside in the expressed protein. It is harmful to animal health. If I...

01 January 1970 397 2 View

What is the best method for finding the binding partners of a secretory protein?

My protein is a secretory protein and I would like to find out what other proteins does it interact with (i.e. membrane proteins in the target cells). Ideally, I would like to screen for both...

01 January 1970 8,116 3 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

Why is serum-free media used when making cancer cell conditioned media?

I am preparing cancer cell conditioned media to study the effects of substances secreted by cancer cells on other cells. In some papers, conditioned media is prepared using FBS-containing media...

11 July 2024 7,063 1 View

Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

How it was possible for Dariusz Prokopowicz to get a Research Interest Score of 11,228 and 2,620,101 reads in ResearchGate?

What is the secret behind the success of the researcher named Dariusz Prokopowicz who has a "Research Interest Score" of 11,228 and has 2,620,101 reads in ResearchGate. How was it possible for him...

06 July 2024 2,963 0 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

Is there any way to avoid albumin contamination in isolated extracellular vesicles?

Hi, I am doing western blotting of isolated extracellular vesicles, and I have albumin and calnexin contamination that I don't know why. Is there any way to avoid it?

16 June 2024 9,907 4 View

What are the laboratory precautions taken by the user when handling cancer cells or cancer cell derived extracellular vesicles?

Please give some insight on this

06 June 2024 2,016 2 View

Organoid with Claustro can be linked to LLM like chatGPT ?

Organoid with Claustro can be linked to LLM Neuroplatform - FinalSpark , finalspark.com/neuroplatform/ can be linked to chatGPT ? What to do with this ? Avatar Pilots ? Studying the case of...

28 May 2024 3,302 0 View

Will ultracentrifugation be replaced as the most widely used method for isolating extracellular vesicles?

Ultracentrifugation is the most widely used method for isolating extracellular vesicles (EVs), despite the significant structural damage that EVs suffer during the process.

16 May 2024 9,432 1 View