I am looking for an EBV-negative immortalised B-cell line(s) with a normal genotype. Do they exist?

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Elena Kashuba

Actually, B-cells could be activated with a mixture of anti-CD40 antibody and IL4. You can get cells growing for 2-3 months for sure. In the A. Moosmann lab the activated B-cells lived even longer:

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0001464

Yegor Vassetzky

Thank you! We'll try this.