Any suggestions on how to measure autophagy in cultured cells? I have used Cyto-ID, but I would like to use another technique in addition.

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Does the soil contamination correction factor for decomposing leaf biomass vary according to space and season?

The question refer to leafy biomass decomposition using litterbag techniques.

11 July 2024 2,078 0 View

What role does grain size play in the mechanical properties of nanocrystalline metals and how can this be controlled during the manufacturing process?

In nanocrystalline metals, the size of their crystalline grains affects how strong, ductile, and hard they are. Smaller grains often mean stronger metals but can reduce ductility. Controlling...

28 June 2024 6,925 0 View

Who will be criminally held responsible for the misdiagnosis and mistreatment of a patient that leads to the death of the patient?

As an advocate and a passionate researcher on AI tools' application in law, healthcare and education, I've being wondering on the criminal liabilities associated with misdiagnosis and mistreatment...

09 December 2023 6,095 2 View

How is AI-induced murder or manslaughter going to be handled in selected common law countries in the world?

AI-induced murder and manslaughter are possibilities that are foreseeable in the not far future from now. How can international communities especially the common law countries going to handle such...

24 November 2023 3,840 6 View

Will AI improve patient outcomes when used at the point of care?

08 November 2023 8,896 1 View

How does AI differ from standard biostatistics? What is “big data”? How does AI enable big dataset analysis?

How does AI differ from standard biostatistics? What is “big data”? How does AI enable big dataset analysis? Can we argue for their synchronization for effective use of these two for efficient...

08 November 2023 8,870 2 View

Does AI enhance or dis-intermediate patient–physician engagement?

08 November 2023 7,259 2 View

Can AI address medical practice “pain points,” providing more efficient and efficacious care while de-escalating physician burnout?

08 November 2023 739 1 View

How do AI applications differ from smart technologies (medical devices, digital diagnostics, data management systems)already used in medical practice?

How do AI applications differ from smart technologies (medical devices, digital diagnostics, data management systems) already used in medical practice? Can we establish some distinct differences...

08 November 2023 5,872 2 View

How is AI use in medical practice distinguished from big data analytics applications for health care delivery and population health?

08 November 2023 8,233 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View