How to set up rheological model (CA and CAM) ?

how to set up asphalt rheological model (CA and CAM) ? How to build EXCEL?

23 September 2020 7,692 2 View

How to improve yield of MACS negative isolation (Mitenyi Biotec)?

29 October 2019 7,134 1 View

Why I cannot claim my article?

19 December 2018 4,086 3 View

How can i set ABAQUS step time period dynamicly?

Hi all, I want to couple a code,named A, with ABAQUS.The code A will give a evoling temperature fields to ABAQUS in every time step corresponding to ABAQUS n step analysis. So,the ABAQUS step-n...

30 July 2017 3,490 1 View

Will PCR product amplified using phusion HIFI DNA polymerase have 5' phosphate groups?

Hi Guys I am trying to clone my PCR product into PUC19 with blunt end ligation. I amplified my PCR product using phusion High-fidelity DNA polymerase (NEB). I cut the vector with SmaI and treated...

04 May 2016 6,084 3 View

How to make the sapphire substrate Al-terminated?

Hi All, I am working on the synthesis of TMD materials on sapphire substrates by CVD. The sapphire substrates that I'm using is O-terminated, but I want to synthesize TMDs on the Al-terminated...

06 September 2015 5,718 2 View

Any suggestions for myc protein westernblot?

There are two sepcific and strong bands when I use anti-myc(santa cruz 9E10) to analysis the whole cell lysates of transfected flag-myc 293t cell.But I could not detect any specific bands when I...

14 June 2015 7,698 6 View

What kind of characterization method can be used to confirm the surface atom of the sapphire substrate?

22 April 2015 6,719 12 View

Is climate science equal to natural science plus polical or social science?

This question has puzzled me for a while.

29 December 2013 6,433 5 View

Is climate science equal to natural science plus polical or social science?

This question has puzzled me for a while.

11 December 2013 9,463 6 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to evaluate unsupervised clustering metrics when some elements do not have a cluster label in the result?

I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...

27 February 2021 428 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View