How long can a gold-labelled EM sample be kept in glutaraldehyde before detected by scanning electron microscopy?

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Why do I have an increasing concentration for increasing dilutions on spectroscopy even though they should all be the same final concentration?

Hello. We have a sample with an unknown concentration of polyphenols. We're diluting the sample in order to perform the Folin Ciocalteau method. 0.2 grams sample / 4 mL 70% methanol and 30%...

10 June 2024 2,134 3 View

Can anyone share a simple rip lysis buffer recipe ?

I have searched for a long time to get a lysis buffer recipe for rip, but the components of them are complicated, If I bought all the ingredients, it would be a waste! And is there any...

26 September 2023 2,612 1 View

Gene editing knockin cells have fluorescence, but pcr has no target band?

May I ask why the cells have fluorescence after the target gene is knocked in by the gene editing method, and the genome is extracted after the monoclone is screened, and then PCR is performed,...

20 August 2023 5,961 3 View

What is the reason for adding an intron between the homologous arms of the donor vector designed by gene editing? Why put the puro gene in the intron?

What is the reason for adding an intron between homologous arms in the donor vector designed by crispr/cas9 gene editing knockout experiment? Why put the puro gene in the intron?

16 August 2023 4,517 2 View

Can donors be inserted into the genome in reverse? What does it do?

Can DNA donors repair template be inserted into the genome in reverse? What does it do?

15 August 2023 5,577 1 View

How to estimate the uncertainty of RUSLE modeling results by stochastic simulations?

The RUSLE is a purely deterministic model in which the product of physical measures is used to derive the amount of soil loss, a rigorous assessment of uncertainties is not feasible, especially...

04 August 2023 2,005 0 View

Are there good quality SSCI full OA journals for educational studies?

I just completed two qualitative studies about educational technology and really hope to get some guidance about which journal to choose. For PhD graduation, I hope my articles can be published...

15 June 2023 4,294 0 View

How do I measure activity of an enzyme if it catalyses a two-step reaction?

How do I measure activity of an enzyme if it catalyses a two-step reaction? The final product is what I want.

18 April 2023 315 7 View

Could someone tell me why many ionic liquids were mixed by v/v despite they actually being solid?

I'm doing some work on mixing hydrophilic ionic liquids with an aqueous buffer as biocatalytic reaction media. As I read the papers, I was aware of some commercial ILs mentioned in the papers were...

05 April 2023 1,504 0 View

Could you determine how many times you have to try before your MS is accepted for publication? Would you prefer traditional journals or new OA ones?

Could you determine whether your newly minted MS will be accepted on the first, second, third, .... or the nth time you have tried?

07 March 2022 2,547 1 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View