Does anyone know if Calcein AM assays have been successfully used on anaerobic cells to measure cell viability/ cell proliferation? A link to literature supporting your answer would be greatly appreciated.
Thanks!
… with my present female lover … she and I spend anywhere from two hours to six hours in caressing, touching, cuddling, hugging, lip kissing, deep kissing and intimate conversation before,...
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Your partner’s not there to service you, it’s not their job to keep you sexually satisfied. You’re together because you love each other and want to make each other happy. Constantly hassling them...
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Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
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Women often complain that their husbands never touch them unless they want sex. (Michele Weiner-Davis)
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Women need to feel a degree of sexual intimacy before sex becomes desirable… For women, intimacy sometimes results in sex; for men, sex sometimes results in intimacy. (Marina Muratore)
31 July 2024 8,860 0 View
Women associate affection with love. … Men associate affection much more directly with sex. … Men see affection of any kind as a sexual invitation. Many women find this bewildering. (Kramer &...
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How to use energy flexibility in inventory modeling?
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Looking for paper collaboration writers: Collaboration Model 1: 1.1 Based on the chosen topic, complete the paper writing, select an SCI or SSCI Q1 journal, submit using my QRCID, and complete...
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Women also often find it easier to fantasise when self-pleasuring than in sex with a partner. The immediacy of someone else’s needs actually inhibits the expression and satisfaction of their own....
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Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
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