How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Any suggestion for conductive paste for PDMS or Ecoflex for electrode?

Dear colleagues, I am trying to make a sturdy electrode on a flexible polymer substrate (PDMS or Ecoflex). I have used a silver paste, but once cured, it peels off very easily. the cured silver...

14 December 2020 4,236 3 View

Can I autoclave d-mannose?

Hello everyone, I was wondering whether I should autoclave or filter d-mannose solution. In our laboratory, we autoclave glucose separately when we make YPD media for yeast culture. So, I am...

08 October 2020 7,068 1 View

Does topology from ATB support FF other than Gromos?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

07 June 2020 8,989 2 View

How can I use a molecule from ATB in Gromacs?

I am new to MD / gromacs and I am trying to use MD not for biological system but for generic materials science. Specifically, I am trying to simulate the interaction between hydrophobic molecules...

06 June 2020 2,112 4 View

Tips on interpreting cell morphology and counting mouse primary aortic smooth muscle cells?

Hello, I recently started culturing mouse primary aortic smooth muscle cells (purchased from Cell Biologics) with the media supplied by the same company (5% FBS and a whole lot of other stuff)....

19 March 2020 9,781 3 View

Does anyone know about analysis of 3D mapping and depth profile for wooden object?

I have 1cm cubic size wood samples. inside of wood, there are S and S-organic, inorganic compounds randomly. I'd like to figure out the quantities of S distribution non-destructively. First, I...

20 February 2020 9,303 2 View

Is there any atmosphere that Titanium chloride can make fume?

I use TiCl4 to check if there's water in the glovebox. when I opened TiCl4 there were a lot of fumes, but I never guess where the water comes. Because there was no oxygen inside. So I didn't sure...

13 January 2020 9,127 1 View

Is it viable to replace ray-traced 3d simulation visualization with Neural network (say GAN)?

My problem may not be very general. Imagine you observe the sun through thick clouds in multiple wavelengths. Given the spectrum of light starting off the sun's surface, you can calculate how rays...

08 January 2020 4,764 1 View

Does accuracy of system identification guarantee the performance of controller in control engineering?

Does the accuracy of system identification affect performance of controlling an object in control engineering? For example, I did system identification and obtained matrix A, B, C, D using...

18 November 2019 4,431 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View