The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
2,387 views 605 posts
Questions related to Histology
Only pubs dealing with the histologic mapping....
03 March 2017 10,034 0 View
Dear colleagues, I am currently looking for used but minimally working microscopy, histology and cytology equipment. Is there anyone who wishes to get rid of old things? E.g. microtomes, tissue...
03 March 2017 882 0 View
We have been trying to section 120hpf fish and are having trouble with maintaining the integrity of the tissue. Also, we use hematoxylin and eosin stains.
03 March 2017 3,207 1 View
Hi, I have been doing slide in situ hybridisation on wax sections (mouse embryos age e10.5) and every time I do it the tissue sections look damaged. Can anyone suggest a solution to improve the...
03 March 2017 9,982 2 View
I want to localize Sialic acid in tissue sections of the skin of certain catfishes. Please suggest some anti Sialic acid antibody which would work in fish skin to do the same.
25 February 2017 2,117 3 View
Dear Colleagues, I would like to learn plant anatomy, I am searching for techniques which will allow me to realize successful and clear microtome slide and how we can preserve them longer.Thank...
03 February 2017 8,905 4 View
02 February 2017 6,083 3 View
HI, Most of protocols for ORO staining of frozen tissue suggest to use Mayers hematoxylin instead of Gill hematoxylin. Would it be possible for me to use Gill hematoxylin as it is currently...
02 February 2017 3,136 10 View
I would like to perform IHC and H&E stainings on spurr (resin) embedded slides, but don't know how to remove the plastic to fully expose the tissue for optimal staining.
02 February 2017 2,765 7 View
I am wondering what other staining protocols are out there that can aid in identifying the populations of neurons in frozen hypothalamic sections. The sections are 40um thick. Unfortunately cresyl...
02 February 2017 8,827 7 View
How I can isolate layer IV projections in prefrontal cortex in rat brain?
02 February 2017 7,208 0 View
Hi, I am looking for someone with experience with travelling with histology sections in their hand luggage on flights within EU. I am in Vienna to perform sectioning and I need to travel back to...
02 February 2017 8,276 7 View
this related to histology research about nerve fibers (peripheral)
02 February 2017 6,776 0 View
I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you
26 January 2017 4,725 6 View
The eye would then be processed for paraffin embedding. We are attempting to improve the morphology of rabbit eyes when using 10% formalin or 4% PFA as fixative. Davidson's has proven to work...
23 January 2017 5,555 2 View
histology
03 January 2017 1,418 4 View
I wanted to compare the effect of partial dehydration of decellularised scaffolds on structure by imaging the surface with SEM and then measure fiber diameter using Image J,, But my problem is...
01 January 2017 1,551 5 View
01 January 2017 9,023 2 View
01 January 2017 3,915 5 View
We have some mouse brain tissue fixed in 4% PFA and embedded in OCT. Can you mount the cryo cut sections immediately onto slides? And can these slides be stored long term in the freezer prior to...
01 January 2017 796 5 View
01 January 2017 4,905 6 View
I have some paraffin sections of mammary gland that have EdU. I have done whole mount staining but would like to know if anyone has a protocol for EdU detection of paraffin sections (rather than...
01 January 2017 5,073 2 View
In tissue processing, xylene is toxic and difficult to regulate end point (especially manual processing). mineral oil - isopropanol combination can be used effectively. but what processor uses...
22 December 2016 4,052 2 View
I observe it in the transmission electron micrographs (the attachments) of mouse intestine.
12 December 2016 8,845 6 View