The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Hi all, I'm making an imaging phantom (as shown in the attachment) that should be sliced by microtome to create a microscopic image, but I believe it will fall apart during the slicing process....
31 May 2018 8,813 7 View
I'm trying to quantify fibrosis on a Picrosirius Red histological image using a macro for ImageJ written by Hadi AM, Mouchaers KTB, Schalij I, et al. Rapid quantification of myocardial fibrosis: a...
28 April 2018 7,737 3 View
I'm having an hard time trying to evaluate diagnostic concordance between lung biopsy histology reports and radiological findings (CT scan or XRay).. Do you know of anyone that has performed this...
14 March 2018 4,605 7 View
Research is being done to determine whether the use of phytogenics in place of growth factor antibiotics is beneficial or not for animals (especially monogastrics)
28 February 2018 7,025 4 View
I currently have a problem. When I immerse skin tissue in O.C.T. and freeze it in liquid nitrogen the cryosections come out great (uninfected skin control) but when I do this exact procedure with...
26 February 2018 8,862 5 View
Hello! I want to know how to clean rodents intestine, especially large intestine where the dregs are more consistent and not easy to evacuate. I usually clean the intestinal lumen in other...
24 February 2018 1,127 3 View
I am planning a Verhoeff van Gieson stain on arterial sections: do they need to be paraffin-embedded or will the stain still work well on frozen sections? I found this protocol...
09 February 2018 827 3 View
Hi I am working with formalin fixed mouse lungs. After fixation in NBF (48h; first intratracheal instillation, then in HistoPot) I am dividing lungs into lobes, and then from each lobe I take...
01 February 2018 8,566 11 View
Hi, I am looking for a protocol to preserve the structure of the skin during the criosectioning. I am trying to visualize the section of a skin sample, with particular interest in the hair...
23 January 2018 9,357 4 View
I am a newly PhD graduate who is looking for postdoctaral research fellowship position. My research interest lies on pig and poultry nutrition. 1. Identifying non-conventional feeds and their...
19 January 2018 9,563 3 View
I have planned to use ZM,GPY, a minimal media, and one rich medium for Bacteria? kindly suggest an addition, if any. For cyanobacteria I am planning to use ASN III medium, kindly suggest an...
30 December 2017 1,028 4 View
As in the question, we couldn't ship our samples in parafomaldehyde between Canada and the US so we put them in saline and shipped them express. They got stuck at the border, and no one realized...
07 December 2017 8,899 7 View
Hello, Is there like any points of identification you can look to to immediately find that the slide is one of the pituitary gland? Thank you
29 November 2017 3,638 4 View
I have human skin equivalent models i have grown onto permeable supports. The tissues are ~20mm in diameter and 1 mm thick. I want to embed the tissue samples so that i can cut cross sections of...
20 November 2017 5,779 9 View
Research is investigating crypt CCO staining on serial colon histology sections. I am enquiring if there is a known software for analysing and 'counting' the percentage of crypt cells that are...
20 November 2017 1,073 2 View
Basically, I'm studying on the reproductive structures of a cyanobacteria encrusting sponge, Terpios hoshinota in Malaysia. However, I'm quite new in this field and I need help from those who are...
05 November 2017 6,322 3 View
We have two Nuaire incubators, a dual-chamber NU-8700 and a single chamber NU-4950 both set to 5% CO2. From the age and schematics they appear to use the same model CO2 sensor. Both incubators...
25 October 2017 7,308 3 View
I'm trying to interprete histological data of hepatocellular carcinoma sections. In some groups more necrosis and hemorrhage is present. Does this correlate with a worse stage HCC or is it a sign...
11 October 2017 2,879 8 View
In one of our experiments we froze down mouse brain tissue with the intent of assessing specific parameters through histology. However, we would like to assess caspase activity in the tissue as...
10 October 2017 2,647 3 View
I am trying to find out whether people use microscope chamber slides. And if so, whether they ever centrifuge such slides, for example in a viral or bacterial infection protocol, for histology...
09 October 2017 3,666 3 View
Normally there are a lot of this cells freely located along the peritoneum in the abdominal cavity and around internal organs (fig1, fig2). But in some specimens there was similar cells in liver...
18 September 2017 6,478 3 View
Hey folks, I have a batch of slides with histological cross sections which were originally embedded in paraffin. They were then sectioned and mounted between 2 and 3 years ago. Now we want to do...
24 August 2017 2,738 14 View
I noticed some unexplainable structures, which are birefringent under polarized light in Ornithodoros. I thought it could be the coxal gland, but its really big? Or is it an artifact, because...
23 August 2017 1,573 4 View
I am looking for a protocol/procedure to obtain ultra thin histological sections that doesn't involve the use of rotary microtome. Please let me know about any other simple (easy-to-follow) and...
19 August 2017 9,544 10 View