The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Hi, We are currently working on grouper liver and observed severe swellings of hepatocytes. Please see attached images of hepatocytes stained with HE and PAS respectively. We tested for...
11 May 2021 4,295 8 View
The European Union is where the world's most peaceful democracies live today. In addition, the member states of the Union are living a future that will bring prosperity through their common...
03 May 2021 5,666 3 View
I was using pump-probe technique to measure the photoinduced polarization rotation of the reflected probe beam from an antiferromagnet. The incident probe beam was linearly polarized. I struggle...
21 April 2021 4,280 1 View
I am given to understand that the root cause of many of the eye problems could be in some way or other, be associated with reduced blood circulation in the eyes. Would some experts in this field...
14 April 2021 8,116 21 View
I tend to prepare percoll dilutions in bulk (40% in HBSS in this case, but occasionally we use 30% and 70%) for large experiments and filter sterilize the finished product with a 0.22µm filter....
25 March 2021 1,346 0 View
In the literature, some part of the articles, regarding the topic about the level of ligation of the inferior mesenteric artery in oncological operations of the sigmoid colon and rectum,...
24 March 2021 6,827 15 View
I have a Si photodiode (hamamatsu S2281 series) in my optical set up (figure attached). Basically the light from the laser (fs-pulsed @ 1550 nm) passes through a Lambda/2 plate (for 1550 nm) and...
11 March 2021 7,002 0 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
02 March 2021 7,487 1 View
I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...
12 February 2021 2,911 2 View
Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...
09 February 2021 7,356 3 View
Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?
03 February 2021 1,327 4 View
Hi, I am looking for pointers on works on detecting and redacting sensitive textual information (such as patient names and identifiers) present in medical images. The images include MRI, CT scans,...
03 February 2021 6,054 4 View
I have a number of small bodied fishes that I want to extract gills from, so as to do morphological analayses and histopathology. The problem is: I have a lot more that I need to do the same work...
29 January 2021 6,495 3 View
Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo
27 January 2021 4,572 3 View
Hi, I am currently undertaking a species distribution modelling project using maxent and RCP predictions. However I am encountering issue where when I put it the required number of replicates (5)...
11 January 2021 4,832 9 View
My thesis is on CRM implementation perceived by skincare practitioners.
30 December 2020 2,273 2 View
in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...
12 December 2020 9,893 3 View
I have to identify lymphocytes on histological sections (after PFA fixation). Please is there any good marker which can be used to label both B and T lymphocytes? Thanks
04 December 2020 1,683 7 View
Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...
02 December 2020 1,417 3 View
Hello, I am curious as to if anyone has any expertise or information about setting up a subcutaneous human xenograft model for a CD19 bearing tumor and human CAR experimental system. I looked up...
01 December 2020 3,177 1 View
Technical question for Research Gate Administrator.
27 November 2020 4,790 3 View
Hello everyone, I plan to begin a project where I want to analyze T-cells transduced with a TCR specific for HER2 after co-incubation with macrophages that present a HER2-derived peptide. After...
19 November 2020 4,267 2 View
Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...
17 November 2020 2,717 3 View
Maybe this is a silly question to ask, but I am still curious. The sandwich was made correctly but at the moment of putting it into the transfer box, the cassette was in the wrong direction (i.e....
05 November 2020 5,048 0 View