Is fluoro-jade staining possible with tissue-cleared brains?

I am hoping to stain whole mouse brain hemispheres with fluoro-jade C as a marker of degeneration. We are presently working to optimize this staining for tissue cleared with the LifeCanvas SHIELD...

10 March 2020 9,709 1 View

Antibacterial liquid inhibition assay (MIC) with LB medium?

Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...

09 February 2020 6,330 5 View

Does it help for a prospective graduate student to have a research proposal in hand when applying to grad school?

This coming semester, I will be teaching an undergraduate class on graduate school preparation for science majors. One of the main assignments during the class will be the assembly of a full...

17 December 2019 8,239 11 View

Looking for validated questionnaires to use with both children and parents on reporting Emotional Health/difficulties?

Hi there, I'm currently doing my MSc in Psychology of Education. I'm looking to answer the following research questions for my dissertation? a) Are there differences in self-reporting of...

13 December 2019 8,779 5 View

Can a cell membrane be overloaded with too many particles attempting to enter?

When more than one biomolecules attempt to enter into a cell membrane at once (in a large quantity) does it impede the transfection process?

23 July 2019 4,149 2 View

How to improve DNA yields using DNA extraction kits designed for soil or sediment samples?

02 November 2017 9,029 3 View

How do you respond to requests for article reprints for articles which were published in journal to which you assigned copyright?

If the publication journal 'owns' the article and the request is not for teaching/educational purposes from someone who is not a current collaborator, do such requests fall under 'fair use' or...

17 October 2017 5,325 3 View

Has anyone conducted research on child protection, self harm, sucide, etc in SE Asia, more specifically Laos if possible?

Looking for any research around child protection in Laos. GoL sensors everything so I'm looking for information that might not have been censored yet. Thank you!

16 August 2017 614 3 View

Can SPSS be used to carry out a Cox PH model with a time-varying exposure covariate that changes status more than once?

I have looked through some help documents that indicate that the time-varying covariate can be constructed for an individual that has been exposed and then unexposed, or vice-versa. I was...

26 July 2017 7,097 3 View

ANOVA versus Student's t-test_Small samples

Hi, I hesitate between two statistical tests (ANOVA and Student's t-test) to compare the means of three groups (unpaired, n

20 July 2017 6,168 6 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

What are the factors on which exchange current density of an electrode depends, and how can they be predicted?

While surface roughness is an obvious factor, this is not what is exactly asked. Rather, periodic variation of exchange current density (of an electrode) with solution chemistry (of electrolyte)...

30 January 2021 4,886 5 View