The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...
18 December 2018 5,939 3 View
histology slid preparation
10 November 2018 5,877 8 View
Spinal cord histology
24 September 2018 9,152 2 View
Hi, I want to do an in-house TSA for IHC using (primary Ab then) a standard biotin conjugated secondary antibody, then avidin-peroxidase, then tyramide-biotin, then avidin peroxidase, then DAB....
22 August 2018 6,306 3 View
It is my first research (Thank should be done by me from A-Z ) and I want to know the new technologies and techniques that used to transfer the histological findings to bio statistical numbers.
10 August 2018 3,888 12 View
Our lab is interested in doing single-unit recordings in area CA2 of the hippocampus. We have a lot of experience with single-unit recording, but not in area CA2. Does anyone have experience...
04 August 2018 8,811 9 View
Hello, I would like to use Neuron Studio in order to quantify dendrite and spine morphological features in 200 um brain sections stained with Golgi-Cox. Neuron Studio software opens TIFF stacks...
01 August 2018 6,863 5 View
I am looking for software that will allow me to layer histology slide scans with corresponding CBCT slices and do measurements and analyze similarities. Does anyone know of one that would do this?
30 July 2018 1,738 2 View
I need to measure the amount of newly formed collagen in primary sutured cutaneous incisions for comparative reasons between different treatments using Hematoxylin and eosin histological...
14 June 2018 6,501 4 View
Hi all, I'm making an imaging phantom (as shown in the attachment) that should be sliced by microtome to create a microscopic image, but I believe it will fall apart during the slicing process....
31 May 2018 8,807 7 View
I'm trying to quantify fibrosis on a Picrosirius Red histological image using a macro for ImageJ written by Hadi AM, Mouchaers KTB, Schalij I, et al. Rapid quantification of myocardial fibrosis: a...
28 April 2018 7,728 3 View
I'm having an hard time trying to evaluate diagnostic concordance between lung biopsy histology reports and radiological findings (CT scan or XRay).. Do you know of anyone that has performed this...
14 March 2018 4,593 7 View
Research is being done to determine whether the use of phytogenics in place of growth factor antibiotics is beneficial or not for animals (especially monogastrics)
28 February 2018 7,014 4 View
Hello! I want to know how to clean rodents intestine, especially large intestine where the dregs are more consistent and not easy to evacuate. I usually clean the intestinal lumen in other...
24 February 2018 1,113 3 View
I am planning a Verhoeff van Gieson stain on arterial sections: do they need to be paraffin-embedded or will the stain still work well on frozen sections? I found this protocol...
09 February 2018 820 3 View
Hi I am working with formalin fixed mouse lungs. After fixation in NBF (48h; first intratracheal instillation, then in HistoPot) I am dividing lungs into lobes, and then from each lobe I take...
01 February 2018 8,554 11 View
Hi, I am looking for a protocol to preserve the structure of the skin during the criosectioning. I am trying to visualize the section of a skin sample, with particular interest in the hair...
23 January 2018 9,349 4 View
As in the question, we couldn't ship our samples in parafomaldehyde between Canada and the US so we put them in saline and shipped them express. They got stuck at the border, and no one realized...
07 December 2017 8,892 7 View
Hello, Is there like any points of identification you can look to to immediately find that the slide is one of the pituitary gland? Thank you
29 November 2017 3,626 4 View
I have human skin equivalent models i have grown onto permeable supports. The tissues are ~20mm in diameter and 1 mm thick. I want to embed the tissue samples so that i can cut cross sections of...
20 November 2017 5,767 9 View
Research is investigating crypt CCO staining on serial colon histology sections. I am enquiring if there is a known software for analysing and 'counting' the percentage of crypt cells that are...
20 November 2017 1,067 2 View
Basically, I'm studying on the reproductive structures of a cyanobacteria encrusting sponge, Terpios hoshinota in Malaysia. However, I'm quite new in this field and I need help from those who are...
05 November 2017 6,314 3 View
I'm trying to interprete histological data of hepatocellular carcinoma sections. In some groups more necrosis and hemorrhage is present. Does this correlate with a worse stage HCC or is it a sign...
11 October 2017 2,872 8 View
In one of our experiments we froze down mouse brain tissue with the intent of assessing specific parameters through histology. However, we would like to assess caspase activity in the tissue as...
10 October 2017 2,630 3 View