The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
I am trying to find out whether people use microscope chamber slides. And if so, whether they ever centrifuge such slides, for example in a viral or bacterial infection protocol, for histology...
09 October 2017 3,662 3 View
Normally there are a lot of this cells freely located along the peritoneum in the abdominal cavity and around internal organs (fig1, fig2). But in some specimens there was similar cells in liver...
18 September 2017 6,468 3 View
Hey folks, I have a batch of slides with histological cross sections which were originally embedded in paraffin. They were then sectioned and mounted between 2 and 3 years ago. Now we want to do...
24 August 2017 2,731 14 View
I noticed some unexplainable structures, which are birefringent under polarized light in Ornithodoros. I thought it could be the coxal gland, but its really big? Or is it an artifact, because...
23 August 2017 1,569 4 View
I am looking for a protocol/procedure to obtain ultra thin histological sections that doesn't involve the use of rotary microtome. Please let me know about any other simple (easy-to-follow) and...
19 August 2017 9,532 10 View
Here is the Reviewer's remarks on my manuscript : Metabolic analysis and cell death monitoring could be reinforced by a second method and / or by analysing metabolic changes in the...
21 July 2017 5,015 1 View
What protocol does everyone use for fixation of tissues prior to putting them in sucrose to prepare them for OCT? I'm using these sections for multiplexing and have issues because the tissue is...
13 July 2017 2,262 5 View
I have some samples ready to be processed for paraffin embedding, but when I cut the decalcified joints into 3mm thickness sections to fit into the paraffin cassettes, the tissues tend to separate...
05 July 2017 8,513 9 View
How can I specifically know the hippocampus over the microscope in the coronal section of the whole brain slice. What have peculiar feature from other brain areas?
22 June 2017 9,179 4 View
New methodology or moderate protocol available for fish histology..... sample fixation, sample processing, staining techniques. or some troubleshooting methods explain thank you...!!!
15 June 2017 7,623 3 View
I am investigating the question of colonic diverticula formation. It was suggested by Mayers et al. that diverticula tend to form in the weak-spots of the muscular layer, where blood vessels...
13 June 2017 2,938 3 View
This may seem like a simple question, but I have never done any form of histology. I have rats with adjuvant-induced arthritis in the left knee joint, and I wish to see the fluorescence from a...
31 May 2017 2,692 14 View
Histopathological changes in different organs are generally assessed to find the health condition of fish.I wish to know which magnification I should see to find if there are any changes in normal...
30 May 2017 3,556 10 View
I have seeded HT29 MTX E12 cells, a human colon adenocarcinoma cell line, into 12 well plate inserts. They are supposed to produce mucus. Now I want to stain the mucus, to monitor mucus production...
29 May 2017 2,969 4 View
Distinct histologic sub-types of breast carcinomas (ie, ductal, medullary, and lobular) suggest distinct etiology and risk factors which in turn call for different preventive and curative...
25 May 2017 3,619 3 View
Hi experts, I have been reading a lot of articles from Abcam, Abnova, Thermo Fisher, etc. about the protocol for IHC. The method seems pretty simple and straightforward. But as I go along the way,...
23 May 2017 8,243 3 View
Hi! I'm doing mouse muscle snap freezing and HE staining. Liquid nitrogen and isopentane were used to snap freeze the muscle tissue. The frozen tissue was then cut with cryostat to get 12um...
16 May 2017 8,290 7 View
Hello, I use X-ray microtomography method to assess vascularization within a motor cortical transplant from cortical lesion of murine model. To do this, I perfuse NaCl, PFA and a contrast agent to...
27 April 2017 370 4 View
Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...
21 April 2017 6,094 3 View
Hello everyone, analyzing the histology of ventricles includes measurement of the ventricle diameter in histological images. Thus I am looking for a method or plugin for either ImageJ or Image-Pro...
11 April 2017 9,612 3 View
Many groups are nowadays trying to stimulate nervous tissue electrically or optically to achieve some intended therapeutical results. However, the usual staining methods for microglia or...
03 April 2017 4,409 3 View
I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the...
22 March 2017 3,243 1 View
I've recently started paraffin sectioning of muscle tissue with some success, however in some sections I find tears in the tissue that all run parallel with each other and I believe run...
03 March 2017 6,643 6 View
03 March 2017 3,623 1 View