The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?
03 February 2021 1,199 4 View
I have a number of small bodied fishes that I want to extract gills from, so as to do morphological analayses and histopathology. The problem is: I have a lot more that I need to do the same work...
29 January 2021 6,404 3 View
Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo
27 January 2021 4,478 3 View
My thesis is on CRM implementation perceived by skincare practitioners.
30 December 2020 2,237 2 View
in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...
12 December 2020 9,842 3 View
I have to identify lymphocytes on histological sections (after PFA fixation). Please is there any good marker which can be used to label both B and T lymphocytes? Thanks
04 December 2020 1,610 7 View
Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...
02 December 2020 1,302 3 View
Technical question for Research Gate Administrator.
27 November 2020 4,733 3 View
Hello everyone, I plan to begin a project where I want to analyze T-cells transduced with a TCR specific for HER2 after co-incubation with macrophages that present a HER2-derived peptide. After...
19 November 2020 4,230 2 View
Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...
17 November 2020 2,662 3 View
i want to know the protocols for H&E Alcian blue method for studying histology of intestine of fish
27 October 2020 6,656 3 View
the most of researches use Rat weighing 200g , is it ok to use rats weighing 100g ? and will it be the same LD50 of heavy metals ( I'm using Arsenic ) and what is the biochemical parameters could...
24 October 2020 3,053 3 View
Dear all, I wonder if it is possible to measure pH in a way that does not require the sample to be fluid. Is there any way one can readout alkalinity? Ideally in mouse histological...
22 October 2020 1,411 5 View
Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...
20 October 2020 8,999 1 View
Hi everyone Do we have a chemical reaction between Ettringite/ Portlandite and a gas mixture of hydrogen/ methane? The pressure is 100 bar and temperature is ambient. tnx
23 September 2020 9,315 0 View
Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...
21 September 2020 2,172 6 View
To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you
18 September 2020 3,213 1 View
I have always used Xylene to stain my slides and also in histology during my PhD. But now, I want to to use some Xylene replacements for staining and in histology too. But I am not sure about the...
19 August 2020 8,792 11 View
I am trying to adapt Sf21 cells to serum-free adherent culture in Gibco® Sf-900™ III SFM. According to the manufacturer protocol, cells will be attached tightly and it will require additional...
04 August 2020 2,069 4 View
View histology svs files on a mac, free to download software.
15 July 2020 6,148 1 View
Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli
06 July 2020 8,254 3 View
I am experiencing wide variation in between the concentration reads from Nanodrop and Qubit Fluorometer for the same samples. The reads are mostly higher from Nanodrop which is sometimes in excess...
25 June 2020 4,883 2 View
I've got myself into a bit of a mess with some analysis. I have conducted an Interrupted Time Series analysis using GLS, but wanted to check whether or not such assumed a normal distribution? The...
19 June 2020 4,242 1 View
Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...
04 June 2020 4,352 5 View