I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View

Is there an influence of terminal cardiac puncture on subsequent brain analysis?

We want to analyse protein expression and metabolomics in mouse brains. The sampling method is snap-freezing in liquid nitrogen. Secondary outcome is blood analysis, which we plan to sample by...

08 December 2020 5,028 2 View

QRT-PCR: DNAse treatment and/or exon overlapping primer required?

How concerned do I have to be about a qRT-PCR experiment in that RNA was not treated with DNAse prior RT and primer pairs are within one exon? Is this a no-no-no or is this usually fine since cDNA...

09 March 2020 7,367 3 View

Ask for help about wannier90, hopping matrix elements are not complex conjugate?

Dear All, I calculate band structure and hopping matrix of monolayer Graphene by using wannier90-2.0.1. There are two atoms in one unit cell, the following is input information : INCAR :...

26 February 2020 4,885 1 View

Why is there a change in EFL measurement in liquid medium?

Hello Everyone, This is regarding the EFL change of a biconvex lens. Can someone explain to me the reason for a change in the measurement of a boconvex lens in water when measured with a time gap...

07 August 2019 7,610 1 View

Has anyone explored the links between yoga and flourishing?

I'm a yoga teacher and MAPP student looking to write my dissertation on the links between yoga practice and flourishing/wellbeing. I'm in the very early stages of looking at this topic.

22 November 2018 3,731 2 View

Anyone has a good recommendation for a study examining the psychometrics properties of CDI-2?

I can only find psychometrics examination of the CDI (the previous edition). I would greatly appreciate it if anyone can share a good study looking at the psychometrics properties of the child's...

08 April 2018 1,667 3 View

Has anyone observed loss of global DNA methylation in HuLy or Tk6 cells without treatment - either due to wrong culturing or during DNA extraction?

I am working in cell culture using Tk6 cells and primary cells (human lymphocytes). Analysing the global DNA methylation with an ELISA I observed only 0.1-0.2 % of global methylation status....

22 January 2018 443 1 View

How have others addressed a large number of 'Not applicable' answers in survey responses?

I have a number of variables being used in the development of a scale. In my survey analysis I've deemed the 'Not applicable' (NA) selections as missing so they are not used in the analysis -...

18 December 2014 5,945 11 View

What are the reasons a PCR that once functioned, is not working anymore?

Last week two Test-PCRs for a new set of primers worked quite well and i got one defined product band. One week later i am not able to get any product again. I exchanged all materials, also the...

25 November 2014 5,733 72 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View