The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
I have tried following several protocols to extract DNA for PCR amplification (Lodhi et al. 1994; Doyle & Doyle1987), from Quercus leaves. Interestingly, some samples showing good and...
03 November 2019 7,355 1 View
Dear Researchers, I'm a veterinarian who ended up in the bioinformatic world as I am analyzing the RNA-Seq using samples from colons of 2 groups of dogs; 1 control group of 3 beagles where I have...
23 October 2019 4,127 2 View
Dear Scholars Help in identifcation of a nematode collected from body cavity of Rita rita, a fresh water fish. sample collected from the Indus river. Images are attached. Regards MMoosa
17 October 2019 2,727 2 View
While using EDTA decalcify bone samples from Mus musculus, how much volume of EDTA is it recomended per piece volume? For the fixation with formaldehyde the normal is arround 20x...
09 October 2019 9,894 3 View
Hi, for biobank purposes we want to store cytospin slides with unfixed cells. What should we pay attention to in order to optimize quality? What is the shelf life when freezing at -80? How to...
08 October 2019 6,642 5 View
Given that the pKa value of 2,4-dichlorophenol is 7.9. At pH 7.2, the 2,4-DCP still does not dissociate as phenoxy ion. The calculated number of electron transfer is 2.
27 September 2019 2,423 0 View
Hi, I have very small samples of about 1x2mm (dental pulp tissue) and I need to process them for histology (dehydration, parafine inclusion, stainning with HE, etc). The problem is that the...
19 September 2019 1,503 4 View
Dear, The HE stain we use is very unstable. When a slide is stained in the morning and the same slide (at a deeper cut) is stained in the afternoon there is a major difference in the color. The...
16 September 2019 4,639 4 View
I have a 3D point cloud. I want to divide these point set on the basis of curvature. i.e. I want to divide them as set of points in a convex region, concave region etc. Can anyone suggest a...
16 September 2019 8,586 5 View
I intend to make flexible OSC using CNT electrodes, so it is possible to use CNT as both the contacts? ( Given that we have electron and hole transfer layer between active layer and contacts)
05 September 2019 3,018 8 View
I am screening antibodies and I discovered that one of them is listed on DSHB (Developmental Studies Hybridoma Bank) as being detergent sensitive. I am wondering what would vary from my normal...
19 August 2019 7,216 3 View
I just realized that my liver tissues may might have hepatocellular carcinoma (HCC) but I'm having difficulty finding any regions of potential HCC. Unfortunately I only have stained for cell...
14 August 2019 4,519 2 View
I want to generate a cell line from a blood sample of a patient that has a very rare type of AML How do I transform malignant cells into a cell line?
30 July 2019 4,820 3 View
I have understood that analyzing genotoxicity with the comet assay depends on which parameter I decide to use, and according to some other publications & scientists, some of them prefer one...
22 July 2019 1,238 4 View
Dear Colleagues! IS it possible to observe oocytes in the adult clam male gonad histological section? The adult clam males were treated for 28 days with high temperature or/and...
15 July 2019 6,463 3 View
Can anyone help me histology TRAP? I'm doing the manufacturer's protocol (SIGMA), and the coloring turns purple. Does anyone know what could be wrong?
10 July 2019 9,211 3 View
hi, does anyone have idea upon measurement of results of comet assay without using a specific software? alternatively could anyone suggest me a free software for quantification of my results?
03 July 2019 6,495 5 View
We are processing pig lungs for ICC. We inflate the lungs with air, then cut a piece and fix it in paraformaldehyde. The next day, we change it to 30% sucrose and wait until the tissue sinks to...
17 June 2019 6,299 1 View
I'm studying a gene, which shows significant poor clinical outcome according to TCGA database in HCC. However, when I investigated its functions, it displayed tumor suppressor phenotype, like...
10 June 2019 9,139 7 View
Searching for talented gene editing researcher in order to have a discution about better understanding the whole process and to start a colaboration. Thank you!
09 June 2019 1,123 1 View
Histological changes in brain
11 May 2019 2,281 4 View
How to distinguish between histological section in the infected kidney and the healthy kidney
02 May 2019 8,487 13 View
We are looking for people who could help us on our way to clinical trials and clinical application of our therapy. We appreciate both financial support as well as close co-operation.
29 April 2019 4,736 1 View
I am trying to calculate the tumor burden of lung by calculating the total tumor area in the lungs by microscopic examination. i have Motic Microscope with its image analysis software (v 2.0). I...
26 April 2019 5,195 0 View