The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Currently, I am doing a research on heavy metals in fresh and salted fishes. Therefore I want to know the latest maximum permissible limits of these heavy metals (Cr, Cd and Pb). The limits which...
06 June 2017 1,074 10 View
This may seem like a simple question, but I have never done any form of histology. I have rats with adjuvant-induced arthritis in the left knee joint, and I wish to see the fluorescence from a...
31 May 2017 2,751 14 View
Histopathological changes in different organs are generally assessed to find the health condition of fish.I wish to know which magnification I should see to find if there are any changes in normal...
30 May 2017 3,612 10 View
I have seeded HT29 MTX E12 cells, a human colon adenocarcinoma cell line, into 12 well plate inserts. They are supposed to produce mucus. Now I want to stain the mucus, to monitor mucus production...
29 May 2017 3,021 4 View
Distinct histologic sub-types of breast carcinomas (ie, ductal, medullary, and lobular) suggest distinct etiology and risk factors which in turn call for different preventive and curative...
25 May 2017 3,665 3 View
Hi experts, I have been reading a lot of articles from Abcam, Abnova, Thermo Fisher, etc. about the protocol for IHC. The method seems pretty simple and straightforward. But as I go along the way,...
23 May 2017 8,304 3 View
Hi! I'm doing mouse muscle snap freezing and HE staining. Liquid nitrogen and isopentane were used to snap freeze the muscle tissue. The frozen tissue was then cut with cryostat to get 12um...
16 May 2017 8,343 7 View
Hello, I use X-ray microtomography method to assess vascularization within a motor cortical transplant from cortical lesion of murine model. To do this, I perfuse NaCl, PFA and a contrast agent to...
27 April 2017 419 4 View
Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...
21 April 2017 6,160 3 View
Hello everyone, analyzing the histology of ventricles includes measurement of the ventricle diameter in histological images. Thus I am looking for a method or plugin for either ImageJ or Image-Pro...
11 April 2017 9,666 3 View
Many groups are nowadays trying to stimulate nervous tissue electrically or optically to achieve some intended therapeutical results. However, the usual staining methods for microglia or...
03 April 2017 4,470 3 View
I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the...
22 March 2017 3,301 1 View
I've recently started paraffin sectioning of muscle tissue with some success, however in some sections I find tears in the tissue that all run parallel with each other and I believe run...
03 March 2017 6,699 6 View
03 March 2017 3,674 1 View
Only pubs dealing with the histologic mapping....
03 March 2017 10,089 0 View
Dear colleagues, I am currently looking for used but minimally working microscopy, histology and cytology equipment. Is there anyone who wishes to get rid of old things? E.g. microtomes, tissue...
03 March 2017 939 0 View
We have been trying to section 120hpf fish and are having trouble with maintaining the integrity of the tissue. Also, we use hematoxylin and eosin stains.
03 March 2017 3,260 1 View
Hi, I have been doing slide in situ hybridisation on wax sections (mouse embryos age e10.5) and every time I do it the tissue sections look damaged. Can anyone suggest a solution to improve the...
03 March 2017 10,029 2 View
How can we proceed intra-operative decision for resecting the a ganglionic segment without the help of a Frozen section? Can we base on the contrast enema?In country we lack of expert in...
01 March 2017 732 6 View
how to identify the cyano bacterial compounds what are their specific key factors , and what is the specific similarities in betwwen cyano bacteria - algal blooms
26 February 2017 1,453 1 View
I want to localize Sialic acid in tissue sections of the skin of certain catfishes. Please suggest some anti Sialic acid antibody which would work in fish skin to do the same.
25 February 2017 2,170 3 View
This is a blood smear of Nile tilapia. What cell is that? Is it common in fish blood? This looks like a myelocyte-neutrophilic. What do you all think?
15 February 2017 1,585 7 View
Dear colleagues, We have planned to generate Human Induced Pluripotencial Stem Cells (IPSCs) from Peripheral Bloods Cells (PBMCs). Nevertheless, nobody in my lab have previous experience in...
09 February 2017 1,404 11 View
Dear Colleagues, I would like to learn plant anatomy, I am searching for techniques which will allow me to realize successful and clear microtome slide and how we can preserve them longer.Thank...
03 February 2017 8,964 4 View