The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Basically, I'm studying on the reproductive structures of a cyanobacteria encrusting sponge, Terpios hoshinota in Malaysia. However, I'm quite new in this field and I need help from those who are...
05 November 2017 6,387 3 View
We have two Nuaire incubators, a dual-chamber NU-8700 and a single chamber NU-4950 both set to 5% CO2. From the age and schematics they appear to use the same model CO2 sensor. Both incubators...
25 October 2017 7,381 3 View
Hi, I have problem with specific markers for fibroblast and myofibroblast for valve interstitial cells. I want to do FACS separtion those two population and culture them. Mainly I though about...
18 October 2017 2,846 2 View
I'm trying to interprete histological data of hepatocellular carcinoma sections. In some groups more necrosis and hemorrhage is present. Does this correlate with a worse stage HCC or is it a sign...
11 October 2017 2,950 8 View
In one of our experiments we froze down mouse brain tissue with the intent of assessing specific parameters through histology. However, we would like to assess caspase activity in the tissue as...
10 October 2017 2,734 3 View
I am trying to find out whether people use microscope chamber slides. And if so, whether they ever centrifuge such slides, for example in a viral or bacterial infection protocol, for histology...
09 October 2017 3,747 3 View
What is the difference between serum urea, plasma urea and blood urea nitrogen??? Are there any calculations to convert the differences among them?
22 September 2017 1,673 5 View
Normally there are a lot of this cells freely located along the peritoneum in the abdominal cavity and around internal organs (fig1, fig2). But in some specimens there was similar cells in liver...
18 September 2017 6,565 3 View
Gastric bypass surgery for morbid obesity is well know to cause a short and long term anemia, vitamin B12 and vitamin D deficiency because of bypassing the duodenum. We receive more and more...
12 September 2017 1,139 5 View
Hey folks, I have a batch of slides with histological cross sections which were originally embedded in paraffin. They were then sectioned and mounted between 2 and 3 years ago. Now we want to do...
24 August 2017 2,809 14 View
I noticed some unexplainable structures, which are birefringent under polarized light in Ornithodoros. I thought it could be the coxal gland, but its really big? Or is it an artifact, because...
23 August 2017 1,644 4 View
I am looking for a protocol/procedure to obtain ultra thin histological sections that doesn't involve the use of rotary microtome. Please let me know about any other simple (easy-to-follow) and...
19 August 2017 9,623 10 View
Anyone aware of any reference article/book/book chapter dealing with the use of dilatometry for estimation of high temperature vacancy concentration in a nanocrystalline/ultrafine phase (e.g. a...
14 August 2017 555 1 View
Which dosimetric term used when the source of radiation exposure is from radioactive material located within the body?
04 August 2017 7,070 9 View
Here is the Reviewer's remarks on my manuscript : Metabolic analysis and cell death monitoring could be reinforced by a second method and / or by analysing metabolic changes in the...
21 July 2017 5,108 1 View
What protocol does everyone use for fixation of tissues prior to putting them in sucrose to prepare them for OCT? I'm using these sections for multiplexing and have issues because the tissue is...
13 July 2017 2,355 5 View
I have some samples ready to be processed for paraffin embedding, but when I cut the decalcified joints into 3mm thickness sections to fit into the paraffin cassettes, the tissues tend to separate...
05 July 2017 8,612 9 View
I request you to please share your research, views, and suggestions.
03 July 2017 9,716 5 View
In multiterminal hvdc load flows , mesh connected type is concerned for a 3 converter terminals (two rectifiers (R1,R2)and one inverter(I)) connections are R1-I and R2-I and R1-R2) R1-I and R2-I...
30 June 2017 6,006 0 View
How can I specifically know the hippocampus over the microscope in the coronal section of the whole brain slice. What have peculiar feature from other brain areas?
22 June 2017 9,263 4 View
Hello I would like to have an help to classify two small unicellular green algae Algae A (i can not understand what it is ...!!!) Small algae that attaches a lot to the glass walls of flask, but...
16 June 2017 5,349 13 View
New methodology or moderate protocol available for fish histology..... sample fixation, sample processing, staining techniques. or some troubleshooting methods explain thank you...!!!
15 June 2017 7,715 3 View
I am investigating the question of colonic diverticula formation. It was suggested by Mayers et al. that diverticula tend to form in the weak-spots of the muscular layer, where blood vessels...
13 June 2017 3,027 3 View
We are trying to use only one plasmid with an inducible Cas9 element for our CRISPR experiments and we would like to introduce both the sgRNA and the donor template as oligonucleotides. We would...
13 June 2017 9,257 3 View