The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
I am looking for an optimal method to perform a Ki67 and C/EBPα staining on frozen sections from adipose tissue. My current protocol exists of the following steps and gives disappointing...
19 February 2016 1,428 2 View
I understand that perfusion is good for getting good histological analyses of adult mouse brains, but what about pups? Let's say below 4 weeks. Do they still need to be perfused? Thanks!
17 February 2016 4,628 3 View
How to stain Chitin molecule in a tissue?
08 February 2016 7,197 1 View
I am using mouse liver tissue which is fixed in 4% PFA and embedded in OCT. However, autofluorescence signal is coming from the negative control samples, when Abcam protocol is used. Do you have...
02 February 2016 8,739 4 View
02 February 2016 4,258 2 View
02 February 2016 2,579 4 View
I did recently glycogen test on liver where we found that glycogen conc. was significantly less than control diet rat. here , we used high carbohydrate high-fat diet rat model. We were expecting...
02 February 2016 570 5 View
uses of interferon injection
02 February 2016 9,485 3 View
Your toughest technical questions will likely get answered within 48 hours on ResearchGate, the professional network for scientists.
02 February 2016 1,177 0 View
02 February 2016 8,331 1 View
in clinical view,I found two common types large and small plaque psoriasis but I Didn't find a histological slides with description on them.
02 February 2016 6,361 0 View
I don't have any idea about dyes >> so If anyone can help me.. I need dye for only carbohydrate in microalgae .. Thank you
02 February 2016 306 3 View
I used two M2 marker (CD206 and CD163) to identify the M2 macrophage but they had quite different expression patterns in histology. Are there different expression spectrums between M2 markers?
02 February 2016 1,746 4 View
My rat golgi stained slices are falling apart during slicing in cryostat (-13°C is the best temperature for me to obtain at least some whole slices) and during color development specially after...
02 February 2016 6,600 2 View
01 February 2016 3,435 3 View
I wonder how to stain for bile acids in tissues?
21 January 2016 7,265 11 View
I have 2 protocols for FISH w/ DNA beacons (approximately 25 nucleotides long) for use on formalin-fixed paraffin embedded tissue (5µm samples) for examination with epifluorescence. One protocol...
06 January 2016 7,048 3 View
01 January 2016 1,554 10 View
Is there any technique associated to histology that allows to detect a mutation in a specific gene of a single cell of a certain tissue? (i dont need to know the type or detail of the mutation,...
01 January 2016 1,177 0 View
I just would like to know a few general tips - What thickness should sections be? What temperature should the cryostat be? Does orientation of the tissue on the chuck matter? Would you recommend a...
01 January 2016 9,218 7 View
01 January 2016 9,777 3 View
As scanning microscopy is comparatively expensive, can stereomicroscopy be used to analyse dentinal tubule morphology ( I agree and understand the detailing is best under SEM) if yes what...
01 January 2016 4,167 6 View
Many articles have been written concerning the presence of the golden number or the numbers of Fibonacci in architecture or botanical sciences. Are there any publications in the field of human...
30 December 2015 2,724 3 View
Im working with fresh apical stems and this organ is very soft. After embed in Cryomatrix or OCT, it is posible to obtain around 40% of sections with good morphology (even cambium cells). But in...
29 December 2015 4,771 1 View