The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
After creating the gastric pouch, a single anastomosis (GJ) is done with a 200 cm loop of intestine. Then do a jejunostomy (Braun type), connecting the afferent loop with the efferent loop. This...
13 June 2017 950 5 View
Currently, I am doing a research on heavy metals in fresh and salted fishes. Therefore I want to know the latest maximum permissible limits of these heavy metals (Cr, Cd and Pb). The limits which...
06 June 2017 1,090 10 View
This may seem like a simple question, but I have never done any form of histology. I have rats with adjuvant-induced arthritis in the left knee joint, and I wish to see the fluorescence from a...
31 May 2017 2,770 14 View
Histopathological changes in different organs are generally assessed to find the health condition of fish.I wish to know which magnification I should see to find if there are any changes in normal...
30 May 2017 3,637 10 View
I have seeded HT29 MTX E12 cells, a human colon adenocarcinoma cell line, into 12 well plate inserts. They are supposed to produce mucus. Now I want to stain the mucus, to monitor mucus production...
29 May 2017 3,040 4 View
Distinct histologic sub-types of breast carcinomas (ie, ductal, medullary, and lobular) suggest distinct etiology and risk factors which in turn call for different preventive and curative...
25 May 2017 3,680 3 View
Hi experts, I have been reading a lot of articles from Abcam, Abnova, Thermo Fisher, etc. about the protocol for IHC. The method seems pretty simple and straightforward. But as I go along the way,...
23 May 2017 8,334 3 View
This is a true branched cyanobacteria that we intend to identify. Anyone there who could give a clear idea of what those yellow colored large cells are?
18 May 2017 1,557 7 View
Hi! I'm doing mouse muscle snap freezing and HE staining. Liquid nitrogen and isopentane were used to snap freeze the muscle tissue. The frozen tissue was then cut with cryostat to get 12um...
16 May 2017 8,362 7 View
can someone suggests me the best way to reduce the background fluorescent in (PFA) lung tissue?
08 May 2017 1,027 2 View
Hello, I use X-ray microtomography method to assess vascularization within a motor cortical transplant from cortical lesion of murine model. To do this, I perfuse NaCl, PFA and a contrast agent to...
27 April 2017 443 4 View
Does anybody know good protocol for rat kidney and eyeball fixation? I have frozen kidney and eyeballs in -80 and want to fix them for IHC. I tried few protocols but eyeball get broken or...
21 April 2017 6,184 3 View
Hello everyone, analyzing the histology of ventricles includes measurement of the ventricle diameter in histological images. Thus I am looking for a method or plugin for either ImageJ or Image-Pro...
11 April 2017 9,695 3 View
Many groups are nowadays trying to stimulate nervous tissue electrically or optically to achieve some intended therapeutical results. However, the usual staining methods for microglia or...
03 April 2017 4,491 3 View
I've been developing a research interest in state and local data sources from working with CHIS for the past few years. We all know about the BRFSS and state-level data like that, but I'm...
27 March 2017 5,088 1 View
I used the cas9 to KO my gene in HEK 293T cell , but one of my control negative cell lines show the increasing of my target protein. I didn't transfect an extra homologous sequence. Seeking for...
24 March 2017 649 0 View
I am studying the histology of mammary glands of lactating Wistar rats. I need to differentiate glands with higher milk secretion from the glands with lesser or no milk secretion. What are the...
22 March 2017 3,319 1 View
I've recently started paraffin sectioning of muscle tissue with some success, however in some sections I find tears in the tissue that all run parallel with each other and I believe run...
03 March 2017 6,714 6 View
03 March 2017 3,695 1 View
Only pubs dealing with the histologic mapping....
03 March 2017 10,113 0 View
Dear colleagues, I am currently looking for used but minimally working microscopy, histology and cytology equipment. Is there anyone who wishes to get rid of old things? E.g. microtomes, tissue...
03 March 2017 959 0 View
We have been trying to section 120hpf fish and are having trouble with maintaining the integrity of the tissue. Also, we use hematoxylin and eosin stains.
03 March 2017 3,279 1 View
Hi, I have been doing slide in situ hybridisation on wax sections (mouse embryos age e10.5) and every time I do it the tissue sections look damaged. Can anyone suggest a solution to improve the...
03 March 2017 10,046 2 View
How can we proceed intra-operative decision for resecting the a ganglionic segment without the help of a Frozen section? Can we base on the contrast enema?In country we lack of expert in...
01 March 2017 761 6 View