How to do rat bone Immunofluorescence, Please suggest?

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Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

To prepare polymethyl methacrylate polymer for the study of acoustic properties without of bubbles during this process, what measures should be taken?

To prepare polymethyl methacrylate polymer for the study of acoustic properties, a mixture of methyl methacrylate monomer and benzoyl peroxide is used. This mixture is then heated and placed on a...

27 June 2024 2,087 1 View

As a university teacher, devise effective teaching methods to enhance students' presentation skills?

ABSTRACT The study explored the relationship between mindfulness levels and academic performance among prospective teachers in the subject of Technical Writing and Presentation Skills at the...

14 June 2024 2,867 2 View

As a Educational Researcher, What is the role of stress Management in the sustainability of learning achievement ?

This article explores the stress levels and their impact on pupil teachers.

15 April 2024 9,273 0 View

Is it proper to conduct 5'Rapid Amplification of cDNA end (by TAKARA) by combining RNA from technical replicates of one biological sample.?

I am about to perform 5'RACE with TAKARA kit on total RNA isolated (trizol method) from neuronal samples differentiated in the same time frame. So I have 3 RNA replicates of one sample, all...

07 April 2024 5,999 4 View

Could you please provide your perspective on some language teaching strategies, as a researcher?

"The article provides valuable insights into language teaching and learning strategies."

15 March 2024 2,772 3 View

In your opinion, what teaching strategies should teachers use to promote intrinsic motivation in students?

I understand how important it is for teachers to promote intrinsic motivation in their students. It can be challenging at times, but with the right teaching strategies, it is achievable. Would you...

10 March 2024 957 4 View

In your country, how you are contributing for Women Empowerment?

Read this article to figure out that Pakistan is contributing for women empowerment and girls education

09 March 2024 7,790 2 View

Is Mind mapping teaching method is a useful teaching method??What do you think ?

This study is about inadequate acquisition of second language in the subject of Expository Writing-I among the graduates of public sector university. The primary purpose of writing exposition is...

09 March 2024 9,746 6 View

Reviewers name in Journal?

Hello Dear researchers I have difficulty in finding reviewers names in Journals during submission of manuscript. Where can I find their names ? Please help Thank you.

28 February 2024 10,077 1 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

Daniel Everett versus Noam Chomsky on Language?

Many have criticized Noam Chomsky’s theory of language (e.g., Pinker as described in Sihombing 2022), but the most effective criticisms have come from Daniel Everett, given that Chomsky (according...

15 July 2024 492 4 View

Aqeel yousif Alshukri

Article Histological and Morphometric Analyses for Rat Carotid Ballo...

Salim Ansari

Dear Sana,

This might help you.

https://www.researchgate.net/post/Help-with-Rat-Long-Bone-Histological-Preparation

Raza Shaheed

Dear Researcher,

https://www.researchgate.net/publication/47681677_A_comparative_microscopic_study_of_human_and_non-human_long_bone_histology