Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Why CHi^2 is reducing if I divide the intensity by a constant factor?

I was refining my XRD data in FullProf. Highest intensity was 400000. The fitting was good but still the Chi^2 value is very high (0.174E+04) I divided the intensity by some constant factor (say...

17 February 2021 3,383 5 View

Is there anyone who is good with DNA extraction from hops?

Hi I extracted the DNA using the CTAB method and purified it with a kit, then I get this band like in the picture below. I am desperate, what can I do!

16 February 2021 5,508 3 View

How pH will effect the overpotential for a reaction like OER,ORR etc?

From a theoretical perspective, when we study the electrocatalytic efficiency of a modeled catalyst, we calculate its overpotential. Here the contribution of pH to energy change for each reaction...

08 December 2020 8,726 3 View

No title

04 October 2020 912 27 View

Why do you think that the body would not have persistent immunity against corona virus after recovering?

why do you think that the body would not have persistent immunity against Corona virus after recovering?

05 August 2020 1,967 3 View

What are the essential things you should learn if you want to be successful?

There are many things we should learn in the way of success, what do you think are they?

23 July 2020 9,832 60 View

Difference between systematic review and meta-analysis?

systematic review and meta-analysis

13 July 2020 4,041 18 View

Incorrect, incomprehensible, illogical answers?

I don't know, why people give 1- 2 sentences answers (even though we are well familiar to those illogical things). OR they will recommend bundles of publications' link which can be downloaded...

01 April 2020 4,776 9 View

Is Grounded theory can be considered as as a research approach as well as qualitative analysis technique?

This question comes to me when my supervisors asked when what analytical technique you have applied for analyzing the qualitative data (interviews and FGDs). As coding is the process and not the...

09 March 2020 2,894 19 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Which database is the most suitable for checking English bigrams frequency?

Which database is the most suitable for checking English bigrams frequency? We want to assess the frequency of different English graphemes. Most of the databases that we have checked seem to...

18 February 2021 4,565 3 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View