Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me to use just 1xPBS to float the section (she uses PBS + triton, but told me I shouldn't begin with that) and then mount it using a brush on the slide, but it's much harder than it looks, and the sections always sink and I screwed many sections because they are so delicate. Do I need to change the solution? I watched many videos for the technique, but I don't know if there's a better solution, or any tips generally.

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