The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
i want to know the protocols for H&E Alcian blue method for studying histology of intestine of fish
27 October 2020 6,808 3 View
the most of researches use Rat weighing 200g , is it ok to use rats weighing 100g ? and will it be the same LD50 of heavy metals ( I'm using Arsenic ) and what is the biochemical parameters could...
24 October 2020 3,105 3 View
Dear all, I wonder if it is possible to measure pH in a way that does not require the sample to be fluid. Is there any way one can readout alkalinity? Ideally in mouse histological...
22 October 2020 1,472 5 View
Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...
20 October 2020 9,055 1 View
Hi everyone Do we have a chemical reaction between Ettringite/ Portlandite and a gas mixture of hydrogen/ methane? The pressure is 100 bar and temperature is ambient. tnx
23 September 2020 9,349 0 View
Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...
21 September 2020 2,228 6 View
To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you
18 September 2020 3,268 1 View
I have always used Xylene to stain my slides and also in histology during my PhD. But now, I want to to use some Xylene replacements for staining and in histology too. But I am not sure about the...
19 August 2020 8,850 11 View
I am trying to adapt Sf21 cells to serum-free adherent culture in Gibco® Sf-900™ III SFM. According to the manufacturer protocol, cells will be attached tightly and it will require additional...
04 August 2020 2,115 4 View
What equipment do we need to measure the linear acceleration energy for the NDT application by the HVL method? For example, what kind of dosimeter linac energy is 6 MeV
25 July 2020 9,583 2 View
I am currently developing a rapid method which involves dual FISH staining of water microbes, the FISH method I am using is a well established method in our lab and we have developed a couple of...
22 July 2020 410 0 View
View histology svs files on a mac, free to download software.
15 July 2020 6,215 1 View
Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli
06 July 2020 8,317 3 View
I am experiencing wide variation in between the concentration reads from Nanodrop and Qubit Fluorometer for the same samples. The reads are mostly higher from Nanodrop which is sometimes in excess...
25 June 2020 4,930 2 View
I've got myself into a bit of a mess with some analysis. I have conducted an Interrupted Time Series analysis using GLS, but wanted to check whether or not such assumed a normal distribution? The...
19 June 2020 4,285 1 View
Not just irregular expression pattern of the proteins in peri-/endo-neurial microvessels, but also a significant higher level of protein expression in distal peripheral nerves of T1DM animals....
19 June 2020 3,867 0 View
Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...
04 June 2020 4,414 5 View
Please tell me why cells with a black nucleus can appear in the parenchyma of the liver and kidney of zebrafish? Is this an artifact of processing? They were found in fish of the control group and...
04 June 2020 7,169 1 View
Generalizability in research endeavors
01 June 2020 564 3 View
H&E histology quantitative analysis using software
18 May 2020 2,633 10 View
Hi, Has anyone applied a transfer function as a means of quantifying an intervention effect in an Interrupted Time Series analysis? I have a time series consisting of 48 quarterly observations...
18 May 2020 1,267 0 View
Based on the survey results, we aim to calibrate the seven episodes; in a way, each one takes a level from 1 to 7 and sort them from the lowest level (1) to the highest level (7) of effectiveness....
16 May 2020 7,761 0 View
I want to manually annotate the CT scan of a spring (3D) to train a U-net. I tried to find tutorials on how to manually annotate and found a lot of examples for medical data. My question is how...
30 April 2020 7,390 0 View
Hi I need to make paraffin blocks to my murine hearts (extracted from 3 month old mice). Following extraction the hearts were held in 4% formaldehyde and 2 days later transferred to 70% EtoH. I...
20 April 2020 7,550 0 View