The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
W made a cross sections of hair and we need to stain them with hematoxilin and eosin, but the problem is that the dry samples are flowing down from the slides...Any idea how to fix them? Thank you...
12 December 2016 8,611 8 View
In tissue processing, xylene is toxic and difficult to regulate end point (especially manual processing). mineral oil - isopropanol combination can be used effectively. but what processor uses...
12 December 2016 798 2 View
Hello, I am freezing "normal" bovine muscle (literally it is steak I got from the grocery store), and the pathologist is telling me that there is freezing artifact. Looking at an H&E, there is...
12 December 2016 3,468 11 View
06 December 2016 262 10 View
Hello I'm Yerin Kim struggling with mouse wound healing research. I've just made full excisional wounds on mouse back and put Tegaderm film(3m) to protect it from infections. And I found that...
16 November 2016 5,570 9 View
I have samples of brain tissues which are over fixed due to prolonged exposure with paraformaldehyde. What can I do for getting appropriate signals in immunohistochemistry on these paraffin...
11 November 2016 4,431 9 View
Is there any differeance in histological structure of orbicularis oris muscle of rabbit and human ?
11 November 2016 890 3 View
I already tried allot of different CD31/CD34 antibody clones to be able to quantify the vascularization of my GMA 8100 fixated tissue. But it did not resulted in any staining. Is there somebody...
20 October 2016 423 6 View
I have been using a 40% Dextran gradient centrifugation protocol with HBSS to isolate mouse capillaries (from cortical homogenate) but have not found any of my pericyte or smooth muscle cell...
17 October 2016 8,221 11 View
Does anybody have experience with preparing the bread as the sample for fluorescence microscopy? What kind of chemicals are the best choice for fixation, embedding and staing?
10 October 2016 3,896 0 View
I do not use embedding cassette - please tell me how it is possible to mount the paraffine block in the clamp like this (in the link) to prevent the block from breaking...
10 October 2016 1,512 1 View
is mitophagy a subtype of autophagy? after reading papers I still do not get a clear anxwer. Some reported that with the increase of overall autopahgy, mitophagy also increases. However, other...
10 October 2016 9,025 5 View
10 October 2016 3,813 7 View
Using the Nottinghan score for tubular differentiation, a tubule Score 1: >75% of tumor area forming glandular/tubular structures, Score 2: 10% to 75% of tumor area forming glandular/tubular...
09 September 2016 3,663 5 View
I am doing frozen section IHC and HE staining. But after all I found my tissue shrinkage. I have several problems. 1. The main problem is on the hematoxylin staining after IHC. When I finished all...
09 September 2016 3,497 5 View
Hi. Im workin on EAE induction in C57BL/6 mice. Im gonna pepare MOG-adjuvant emulsion but i don’t know the storage conditions of it. Some papers suggest storage duration for severeal days but i...
09 September 2016 1,520 1 View
Hi everyone, I will be excising tumor samples from my mice and would like to preserve them for 3-5 years (in case they are needed for histology studies by someone else in the lab). What fixative...
09 September 2016 10,041 3 View
Im trying to use it with cow tissue, but I don't know if Im doing the protocol right.
25 August 2016 3,559 1 View
Kindly check histology of osseointegration
08 August 2016 1,732 0 View
Good morning everyone, I' m about to perform some histologies on some spleen samples. While embedding my samples, I decided to split them in two groups: in one case I will have cross sections, and...
08 August 2016 5,900 6 View
These are two images of hippocampus of rats treated with aluminium. Any idea of what these structures are?. Thank you very much in advance.
08 August 2016 1,483 11 View
I have done histology of animal tissues more precisely fish tissues i.e GI tract brain and liver. I need to know which is best tool to measure the size/dimensions of the tissues. Best Regards Imrana
08 August 2016 222 7 View
Dear colleagues, could you tell me how long does Euparal medium take to dry completely with your samples if you use this medium? I found something like "let it dry several days", but no mention...
08 August 2016 6,941 2 View
Hi, I need to quantify renal and cardiac fibrosis in histology sections and am trying to set up our Olympus Bx43 microscope to analyse the birefringent collagen with picrosirius red stained images...
08 August 2016 5,498 2 View