Can anyone help me, i am trying to calculate mean linear intercept in my histological slides ?

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What do you know about biotechnology art?

02 March 2021 1,653 3 View

Can anyone help me with the protocol of intranasal administration to mice ?

i wanna administer cigarette smoke extract to induce emphysema in mice, i am asking for the maximum volume of intranasal administration ? can i use ether or chlorofom to anesthetize mice?

10 December 2020 6,319 4 View

Links of articles in Google Scholar?

In a scholar profile, in google scholar, when clicking on a link of an article for example, why some articles have a description and link to the page of the journal that publishes that article...

16 November 2020 5,216 4 View

Have you a word or LaTex Elsevier template?

Hello everyone, Hope you are very well. I just wonder if there is a word or LaTex Elsevier template for these two...

06 November 2020 4,504 3 View

How we can design hybrid system between deep learning and swarm intelligence system or deep learning with fuzz?

Hybride intelligent system is good techniques used for solving diffrent optomization problems. Like classification, recognition ....etc Deep learning can be used with and hybrid with other machine...

08 October 2020 4,943 3 View

Acidic hydrogel kills cells?

As hydrogel was prepared in acidic medium, released acid from hydrogel kills cells in in-vitro test. I am wondering to compromise such issue.

12 August 2020 9,319 3 View

Western blot : Empty well presenting signal ?

Dear all, I am currently performing some western blots in a big wells gel (40ul capacity) and i am filling 40ul in each well. My problem is that two of the last wells supposed to be empty...

10 August 2020 4,613 4 View

Mention the name of parasitic worm that causes river blindness?

mention the name of parasitic worm that causes river blindness?

04 July 2020 6,066 8 View

How can I create pre- and post-holiday dummy variables in EViews or Stata?

I have daily closing values of multiple stock indices and i have lists of public holidays for the whole period. I want to inspect the existence of a holiday effect. My regression equation is as...

23 May 2020 5,456 12 View

Where to get Microglia N9 Cell line ?

Hello ! Does anyone know where i can find the following Cell line : Microglia N9 ? Many thanks in advance.

24 March 2020 7,418 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

08 February 2021 6,880 3 View

How to fix and protect blood clots for histology ?

Hi, I encounter some problems, I need to my research to fix and cryoprotec some blood clot for histology analyses. Mys current protocol is the following : 48h of para formaldehyde 4% and 7 days of...

07 February 2021 3,756 3 View