Can anyone help me, i am trying to calculate mean linear intercept in my histological slides ?

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Could the small transmembrane domain of the recombination protein from the virus be the reason for the lack of expression in E. coli?

I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...

20 May 2024 8,161 2 View

What international action should be taken against a number of Indian researchers who stole my research?

My research was published in the Italian magazine Scopus Q2 in 2021. The attachments are my research and the research of Indian researchers in 2023.

01 April 2024 5,153 3 View

How can I measure Audit risk assessment?

I am doing research, and one of my research variables is audit risk assessment. I want to do applied studies instead of questionnaires.

18 March 2024 9,997 3 View

What are these colonies on the EMB agar?

isolated from water and sediments samples.

05 February 2024 5,918 0 View

What is the ambition of educational systems towards future generations ?

What is expected of future generations towards society and the nation ? What are the trends that generations aim to achieve ? for example a peaceful generation .. believes in values.. a...

02 January 2024 7,324 2 View

Have any one tried to partition the system into microgrids using graph theory in gams?

there is a problem in my code and i dont know what is it ? if there is available code can you help me with it

17 December 2023 7,151 0 View

What is the citation related to Time Series Research Design in Educational Research?

Explanation of Time Series Research Design

19 November 2023 5,721 2 View

What is the difference between quantitative and statistical methods in scientific research??

Among the tools of scientific research, quantitative and statistics..

12 September 2023 2,367 4 View

Why I have 2 bands after PCR and gel purification?

I am amplifying gene (1488 bp) from RNA genome using specific primers and run in reverse transcription PCR(RT-PCR). The result, I am getting 2 bands my target band (1488bp) and extra band with...

01 September 2023 4,965 7 View

What are the implications of having infected samples on postprocessing, particularly western blotting??

Hi, I has a question regarding infection models- If you are inducing an infection model (bacterial) and then snap freezing the infected tissues in liquid nitrogen afterwards, does the pathogen...

18 July 2023 4,672 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

How to use ImageJ for fluorescence quantification of platelet adhesion and spreading?

I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...

11 July 2024 1,464 7 View

Why I have this weird green signal in the nucleus?

Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...

10 July 2024 1,870 0 View

Does my paper explain the process of entanglement without resorting to retro causality or infinite branching universes?

In my paper, "Emergent Universe from Many Unreal World Interpretation ," I propose a novel interpretation of quantum mechanics that addresses the process of entanglement without relying on retro...

29 June 2024 10,045 5 View

Hello.. please how can I prepare an emulsion with nile red and nile blue dyes for visualization under fluorescent microscope ?

how can I incorporate the dyes to each phase ? do I have to heat them !!

21 June 2024 9,377 1 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View