The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Recently I have a group discussion about formulating a drug for Hepatocellular Carcinoma(HCC). My group agreed on using CRISPR/Cas 9 system to cure HCC, because theoretically it is more specific...
19 April 2020 6,233 0 View
I am trying to determine if there are still intact cells within dried, archived fish scales so that I can extract DNA. Will I be able to use an H&E stain to determine this and if so, how would...
30 March 2020 8,485 5 View
Hello please could I ask for a second opinion as to what this is present on the small intestine of the rat histological section? I believe it could be a neoplasm of the -intestinal-epithelial...
28 March 2020 8,346 6 View
1) How do I dissolve the Sirius Direct 80 Red? I know it should be in saturated Picric acid solution. But how many grams of picric acid should I add to dissolve in water? What should the...
23 March 2020 609 3 View
What do you think about orthodontic forces on root filled tooth? Have you ever noticed any differences between this case and controlateral vital tooth? In literature, the most part of studies is...
13 March 2020 9,919 16 View
Hi, I was wondering if someone can recommend a stain where fish EGCs or mast cells show significant metachromasia. Toluidine blue did not work in my fish species. I did find recommendations on...
05 March 2020 8,144 1 View
Hello, I need to stain my fish larvae sections (embedded in Technovit 7100 resin) with the methylene blue/azure II/basic fuchsin stain. I have this recipe: Polychrome I 0.325 g Methylene...
05 March 2020 7,509 13 View
Dear researchers, Can anyone help me to identify these odd mammary glands in Ornithorynchus anaticus ? I struggle to understand its mecanism : The secretion from all these flat lobes have a...
04 March 2020 8,582 2 View
Someone know (or use) a CRM of natural water with very low level of Hg (from 20 to 100 ng/l)? I found this two, somebody use ones?...
01 March 2020 1,062 2 View
Histological, X-ray or Endoscopic images, for example.
14 February 2020 3,902 3 View
For some reason our tissue processor had stopped working during last night, leaving my samples (different murine tissues fixed in 4% PFA o/n) in the air before the last ABS-ethanol. I put the...
21 January 2020 822 2 View
I immunostained PDGFRb which should be mainly positive on peicytes of vessel walls in glioma xenografts from mice. However, non-specific signals are frequently seen in vessel lumens. For tissue...
20 January 2020 6,251 3 View
Ever since the eukaryotes entered the aerobic environment, they couldn't survive a moment without oxygen. They choose oxygen than anything else. But why?
09 January 2020 3,247 0 View
What are the histological and physiologically relevant differences between mouse and human brain with respect to the CSF Flow !
05 January 2020 5,395 3 View
In histological examination, l found the diferences in distibution of panceatic cells in the pancreatic islets in the camel and buffalo
29 December 2019 7,019 1 View
Hello guys, So I am trying clone my insert (promoter+gene of interest) into my lenti transfer vector. My insert size is 7200bp and the lenti transfer vector I am using is pLenti-Puro (Addgene...
16 December 2019 3,747 0 View
Are you aware of the best book recommendations by bookauthority? 14 Best New Pediatrics Books To Read In 2020 https://bookauthority.org/books/new-pediatrics-books
14 December 2019 9,431 0 View
Did anyone used Chlorine dioxide based fumigation or disinfection for Biosafety level 2 laboratories? If so what is its effectiveness when compared to Hydrogen peroxide based...
12 December 2019 8,290 0 View
I'm writing a manuscript about histology of a marine fish and I come across various way of presenting (e.g. mode, diameter, component) and this makes me think which is the best.
03 December 2019 8,836 3 View
New transplant programme starting in a small African nation, but they will need support and training in the examination of biopsies. A digital 'whole slide' scanner would allow virtual biopsy...
28 November 2019 10,199 1 View
Can anyone recommend me a method or software to quantify the average amount of PI staining in tumorsphere relative to the size of the tumorsphere by using confocal images?
13 November 2019 4,261 1 View
Cyanobacteria are an ancient group of photosynthetic organisms from different habitats and particularly marine cyanobacteria, are a great resource of bio-active secondary metabolites. These...
10 November 2019 5,107 7 View
I have a problem with sectioning brains on a Leica cyrostat. We are trying to take 20 um coronal sections (which we have had no problem doing in the past) but it looks like the cryostat is taking...
04 November 2019 5,903 2 View
We have obtained some electron micrographs showing what appears to be developmental stages of some type of small eukaryotic organism colonizing/parasitizing the tissues of mammalian hosts....
04 November 2019 2,377 2 View