Hello,

I am curious as to if anyone has any expertise or information about setting up a subcutaneous human xenograft model for a CD19 bearing tumor and human CAR experimental system. I looked up many different references using either the NALM6 or Raji cell lines at varying log differences in cells injected with and without matrigel. We piloted a tumor growth study using the range of cells published (10^4-10^6) without Matrigel and did not get any tumor growth in the time frame (21 days) of the published reports. Any tricks or hints on sublcones for the NALM6 and/or Raji cells would be helpful. The pilot was performed with the NALM6 CRL-3273 from ATCC. Thanks.