The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
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Questions related to Histology
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
02 March 2021 7,149 1 View
I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...
12 February 2021 2,582 2 View
Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...
09 February 2021 7,037 3 View
Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?
03 February 2021 998 4 View
I have a number of small bodied fishes that I want to extract gills from, so as to do morphological analayses and histopathology. The problem is: I have a lot more that I need to do the same work...
29 January 2021 6,239 3 View
Hello, I have been trying to do a rat bone Immunofluorescence histology study. But not able to find a successful protocol till now, Any suggestions? Rat bone: Decalcifies Embedding: Cryo
27 January 2021 4,308 3 View
in the literature, in order to calculate this parameter , they use slide images with 20 microscopic magnification , but in our lab , we don't have this kind of microscope , can I use 10...
12 December 2020 9,692 3 View
I have to identify lymphocytes on histological sections (after PFA fixation). Please is there any good marker which can be used to label both B and T lymphocytes? Thanks
04 December 2020 1,445 7 View
Hello You can see a transversal slice of a mouse heart in microscopie with blue coloration. My question : what part of the heart is it ? how i can recognize difference between histology of...
02 December 2020 1,148 3 View
Hi everybody, I am beginning with the inclusion of biological samples (fish larvae) in resin (i.e. Technovit 7100). I am experimenting some troubles during the cut at the microtome (I would like...
17 November 2020 2,520 3 View
i want to know the protocols for H&E Alcian blue method for studying histology of intestine of fish
27 October 2020 6,500 3 View
the most of researches use Rat weighing 200g , is it ok to use rats weighing 100g ? and will it be the same LD50 of heavy metals ( I'm using Arsenic ) and what is the biochemical parameters could...
24 October 2020 2,927 3 View
Dear all, I wonder if it is possible to measure pH in a way that does not require the sample to be fluid. Is there any way one can readout alkalinity? Ideally in mouse histological...
22 October 2020 1,249 5 View
Hi, I am trying to create a histology based finite element model of human soft tissue. In order to model the viscoelasticity at the cellular level, I need to know the dynamic and bulk viscosity of...
20 October 2020 8,854 1 View
Hello, I am currently conducting a lineage tracing experiment where I need to count GFP+ cells in multiple compartments of ductal epithelium (frozen tissue sections of an inducible Cre mouse with...
21 September 2020 2,002 6 View
To detect by WB, I would have to isolate the nucleus fraction, right? But I did whole protein isolation. Has anyone detected ATF4 or ATF6 by histology? Thank you
18 September 2020 3,083 1 View
I have always used Xylene to stain my slides and also in histology during my PhD. But now, I want to to use some Xylene replacements for staining and in histology too. But I am not sure about the...
19 August 2020 8,690 11 View
View histology svs files on a mac, free to download software.
15 July 2020 5,975 1 View
Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli
06 July 2020 8,076 3 View
Please help determine what cells are in the proximal tubules of zebrafish. They have highly condensed nuclei, but I suspect that this is not karyopiches of apoptotic cells, but mitosis of normal...
04 June 2020 4,214 5 View
H&E histology quantitative analysis using software
18 May 2020 2,414 10 View
I am trying to determine if there are still intact cells within dried, archived fish scales so that I can extract DNA. Will I be able to use an H&E stain to determine this and if so, how would...
30 March 2020 8,334 5 View
1) How do I dissolve the Sirius Direct 80 Red? I know it should be in saturated Picric acid solution. But how many grams of picric acid should I add to dissolve in water? What should the...
23 March 2020 391 3 View
Hi, I was wondering if someone can recommend a stain where fish EGCs or mast cells show significant metachromasia. Toluidine blue did not work in my fish species. I did find recommendations on...
05 March 2020 7,950 1 View