The study of the microscopic anatomy of cells and tissues of plants and animals, including tissue fixing, fixation and staining. | Contact experts in Histology to get answers
2,638 views 688 posts
Questions related to Histology
I am investigating how acid treatment affects the allergenicity of natural shrimp tropomyosin. Based on my reading of existing scholarly literature, researchers typically submerge whole shrimp at...
07 July 2023 3,528 1 View
Need some suggestions I am looking for my research direction on mangrove ecology the gaps that are still not well studied.
23 May 2023 3,483 2 View
The end of the Cold War era between the poles of international powers and competitive displays of conventional arsenals of armaments, including weapons of mass destruction and nuclear weapons,...
01 May 2023 6,335 3 View
I've tried amplifying a single band using matK primers for a while. The plant I'm dealing with also has many metabolites, including polyphenols. matK primers are also known to amplify...
29 April 2023 9,158 4 View
I am trying to decipher the effect of some compounds at the level of chromatin condensation. I have looked at relaxing chromatin with HDAC inhibitors (eg TSA, VPA), which has proven to work very...
22 April 2023 4,122 2 View
Trying a new way to store human brains. I do not have experience with PEG. Any idea how the tissue appears next to tissue stored in anti-freeze? Thanks
10 April 2023 4,028 0 View
While analyzing blood smears from T. hermanni, I have found some changes in erythrocyte cytoplasm and I'm not sure what could it be. It doesn't look like any of the hemoparasites from available...
05 April 2023 1,581 2 View
I am using Aperio positive pixel count algorithm to measure the stain in my renal tissue but I am not able to define the parameters for the negative pixels. Am I doing something wrong?
24 March 2023 8,918 0 View
Muscle frozen sections were stained with antibody against Laminin.
23 March 2023 265 2 View
Hello, What software is most suitable for the automatic analysis of iPSCs neurons images (we are using ZEISS Cell Discoverer 7 for fluorescence imaging)? We are particularly interested in...
16 March 2023 3,280 0 View
I want to specifically visualize Granule cells of the Hippocampus and I wonder if there is a reporter mouse line exists for that? Thanks in advance.
09 March 2023 304 1 View
Hi everybody, I am currently conducting some pilot experiments where I perform intratracheal instillations of LPS in rats to induce pulmonary inflammation. My plan is to examine some...
02 March 2023 6,388 3 View
Liver histology
01 March 2023 2,299 3 View
Is there any specific protocol to extract arcuate nucleus from rat hypothalamus using microtome, keeping cells alive for primary culture?
24 February 2023 1,467 0 View
I want to analyze the dendritic diameter of Granul neurons in the hippocampus (in DG specifically) I wonder if there's specific marker than stains the whole neuron with dendrites and processes?
14 February 2023 8,984 1 View
I do qPCR often using the SYBR green. Everytime, I have to prepare the mix which contains the Primers, Water and the SYBR green mix. I wonder if this mix is stable in -20? Can I prepare a stock of...
07 February 2023 728 0 View
Hi, Can I use 37% formaldehyde (~10% methanol) to prepare 4% formaldehyde sol. in PBS for mice perfusion? How methanol can affect immunohistochemistry?
24 January 2023 7,437 2 View
What are the proper conditions to fix isolated mouse retina (fixative solution, time, temperature) before embedding into OCT prior to cryo-sectioning?
13 January 2023 454 2 View
Dear all! now im working on my thesis (Anti Cancer Effect of Multi walled carbon Nanotubes ). im using SH-SY5Y neuroblastoma cell line and i need a normal neuronal cell line in order to test the...
22 December 2022 4,522 2 View
FFPE tissue sections of various murine tissues including the heart and liver, when stained using Polysciences' PSR kit (Cat# 24901), display 'blotches' or regions where there is a clear difference...
08 December 2022 7,334 3 View
Can anyone suggest me a pre-sorting and post-sorting buffer for H9C2 cardiomyocytes cell lines? I'm using BD Facs Melody Thanx
30 November 2022 6,813 0 View
I accidentally used DSG instead of DSP to crosslink my proteins for a co-IP. Can I decrosslink the DSG crosslinks so that I can run the co-IP's on a western blot for enrichment, that would show...
12 November 2022 1,436 2 View
Hello everyone, I will be using hydrogen gas at 5 bar pressure, Room temperature and 12 l/min flow-rate and 50 liter cylinder. Since the lower limit of Hydrogen is 4% by Volume in Air to form an...
26 August 2022 918 1 View
Preparation plate for gut whole-mounts We used this (picture 1.) stuff to prepare whole-mounts samples from gut. We didn't find what it's the official name. Do somebody know its name? And where...
21 April 2022 8,037 0 View