Hello,
I am trying to encapsulate a hydrophobic drug (that is only soluble in DMSO) in Micelles. The Micelles preparation method is emulsion-solvent evaporation.
After mixing the drug/DMSO and copolymer/DCM, water added and the sample has ultrasonicated.
Now, i want to remove the DMSO from the solution and measure the encapsulation effeciency from the remain non-encapsulated drug.
as long as DMSO can not be removed by rotary evaporation, how to remove it and leave only the micelles dispersed in water ?
Majdi Al-Amili
I report the standard method of placing the drug into a micelle (liposome) from the laboratory work of students. Check your actions.
Preparation of liposomes by manual shaking
1. Dissolve lecithin in an amount of 100 mg in 50 ml of chloroform in round bottom flask (per 200 ml) of a rotary evaporator. 2. Attach the flask to the rotary evaporator and at low pressure generated by a vacuum pump organic solvent. In this case, regulate the pressure so that it does not boil.
3. After complete removal of the organic solvent on the walls a thin film of lipids should form in the flask. Into the flask with a film add 5 ml of a buffer solution containing insulin, and stand 1-2 hours at room temperature for swelling phospholipid films.
4. Then shake the flask mechanically for several minutes. To do this, add glass beads to it and intensively mix the contents of the flask with a high-speed mixer. Wherein a thick suspension of milky white liposomes is formed.
If the drug to be loaded into micelles (liposome) is hydrophobic, you should dissolve it with the lipid using choroform at the stage the lipid film formation then evaporation to remove organic solvent .In other hand ,If the drug is hydrophilic, it is added at the stage of hydration with water or buffer
I think, chloroform will be the good choice with your drug..
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