I need help to dissolve my hydrogel beads for antimicrobial test against gram +ve and gram -ve bacteria, I tried with water and DMSO but cant dissolve even with sonication for two hours but not dissolve. I used PBS (pH7.4) but it will take two days to dissolve. Also I used sodium citrate in PBS and it dissolved, however it gave negative results in antimicrobial test. Note that this hydrogel beads prepared from materials have antimicrobial activity. see the photos
dear D. Shanmuga Priya ,
do you think this kind of solvent is good for antimicrobial test?
I recommend ethyl acetate and you must put a control disc..
Also in my previous research , I used the beads with the same diameter of the disc. But the problem that you will face is the concentration of the formula loaded on the bead. But you can calculate it.
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