Does anyone know of a procedure they can recommend to freeze microlgia?

William Macintosh-Smith @William-Macintosh-Smith

21 January 2020 1 3K Report

Looking to design a protocol for freezing rat microglia, but have found little material in the literature. So far I have only found some papers recommending freezing in DMSO or glycerol for similar cell lines. Does anyone have any advice they can offer to design such a protocol?

Duried Alwazeer

Hi William,

I have no idea about the microglia, but in microbiology we preserve the bacterial strains by using glycerol solutions (15-50%).

Best wishes

Duried

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