I don't get a synthesis sample by pcr, what could I modify? considering that I already started from a standard protocol, I still don't get samples?

More Adriana Albanese's questions See All

Does anyone have this article in PDF and could they pass it on to me?

Paper: Wang, A.G., Luo, G.H., 1990. Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol. Commun. 6, 55–57.

19 June 2024 7,283 0 View

¿Qué equipo de resonancia de plasmones de superficie recomendarían y cual no para mediciones de interacciones péptido-proteína y péptido-célula?

He visto varias referencias de Biacore y todas mencionan sus ventajas. Sin embargo, nunca he usado uno, por lo que me gustaría recibir recomendaciones de personas que conozcan la técnica y que la...

16 April 2024 4,774 1 View

Are SPL 96 well white plates autoclavable?

Hello, my question is if SPL well white plates (30196) are autoclavable? I didn't find any information in Technical Data Sheet. Thank you for your answers.

25 March 2024 9,903 2 View

What is my ID Research Gate nomber?

Where i cant find my ID Research Gate number?

17 November 2023 1,104 0 View

Manders' Coefficient for Colocalization, tM1 or M1 value?

Hi there, I am trying to assess colocalization (overlap) of two signals. The experiments were performed on FFPE human brain aged tissue samples, which means I have a lot of background after...

04 October 2023 2,877 2 View

How to make an RNase decontamination reagent?

Hello, I hope you are well, I have been searching and I cannot find the components to make an RNase decontamination reagent in the laboratory, without having to buy the RNase away. Thanks

26 September 2023 5,360 1 View

Which models can I use to build growth curves for gmelina arborea?

I am working on the development of growth curves for Gmelina arborea for the northern and Caribbean region of Costa Rica, I am interested in knowing what models you recommend for its construction

20 June 2023 279 0 View

No bands in electrophoresis?

Hello, I'm doing a SOE PCR. As soon as the PCR is done, I run electrophoresis of the samples. The gel shows the marker but no sample, as if I didn't load the sample with the loading buffer. But...

27 April 2023 6,495 9 View

Does anyone have tips on technologies that can capture images and voice of pets?

I'm starting a research on technologies that can capture images and voice of pets, according to your behavior. Can anyone give me tips for articles or tools that help me in this study?

23 April 2023 8,190 2 View

K-40 from K total determined by ICP MS in sediment samples?

Is it possible to mathematically calculate K-40 from K total determined by ICP MS in sediment samples?

31 March 2023 5,428 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

Hi dear researchers, do you suggest the best and shortest path for chemical synthesis and green synthesis of magnetite(Fe3O4)?

Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...

21 July 2024 2,476 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

A twin-shaft kiln or a rotary kiln for lime production 1 class? if compressive strength of limestone is low 60 MPa?

My research work is related to the calcination of limestone. I have a complex question and would like to consult with experts in this field. Could you please advise which kiln to choose (a...

18 July 2024 1,390 2 View

Im trying iodination of an arene, but only getting black precipitates, is there anyone with iodination experience who can advise?

Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...

16 July 2024 6,630 1 View

What could be the possible reason that sintering of polycrystalline diamond fabricated with tape casting has suddenly started failing?

I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...

16 July 2024 3,212 5 View

Kraft lignin and MMA(Methyl meth acrylate) free radical polymerization for synthesis of thermoplastics?

I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...

15 July 2024 9,827 1 View

Johan De meester

Dear Adriana,

Did you run also a positive control ?

Here is a video article that might help you out : Article Polymerase Chain Reaction: Basic Protocol Plus Troubleshooti...

Adriana Albanese

Hi Joan, if I used positive control and still .. nothing !! many for everything,

I consider this work that you sent me