Hello all,
We are currently working on angiotensin analysis, however we observed that, once the freeze-dried peptide is dissolved in an aqueous buffer, it gets unstable in only 2-3 hours. Mass spectrometry analysis of the peptide shows us that the peak intensities drop by half, only after 1 freeze-thaw cycle or 5 hours of storage on ice.
I've been trying to find a way to improve the stability of these peptides in buffers that I use for aliquoting, however I couldn't find much information in papers. For example, do you thing adding low amounts of DMSO of glycerol in the aqueous aliquots would keep them more stable? Or, agents which reduce oxidation?
Please look at the following below links which may help you in your analysis:
https://link.springer.com/content/pdf/10.1007%2Fs10989-018-9773-y.pdf
https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/6/a9525dat.pdf
https://www.journalofdairyscience.org/article/S0022-0302(16)30052-2/pdf
Thanks
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