How do I calculate IC50 of a drug towards OCI AML2 cell line?

Hi, I am in the process of learning to calculate IC50 values of OCI AML2 cells for Cytarabine. I have taken the drug concentrations as 0.01 uM to 50uM. My question is while calculating IC50 is it...

18 December 2019 3,639 3 View

What is the appropriate reagent to dissolve formazan crystals in an MTT assay?

Hi, I am performing an MTT assay on HL60 and OCI AML2 cell lines,to detect the IC50 values for cytarabine. I needed to find the appropriate cell seeding density for which i initially seeded 0.1...

03 December 2019 8,862 3 View

What methods can be used to measure suitability of an ecosystem based on plankton population?

I intend to understand how sustainable an aquatic ecosystem is based on its plankton population. Can anyone help me with suitable methods available?

19 February 2019 3,838 13 View

Would anyone know of a scale that maps paternal involvement or attachment/parenting practices? esp. one that can be used in a non-western context?

03 August 2016 950 6 View

To analyse thin filem using SEM which substrate is preferred? What should be the size and thickness of it?

For forming thin film of metal doped nano TiO2, glass or Silicon surface is preferred and of what size?

26 April 2016 6,656 6 View

How to fine lattice parameters using XRD data?

22 February 2016 5,648 5 View

Does anyone know of a short questionnaire to assess child attachment or parenting style?

Child attachment related brief tools

30 July 2013 9,530 12 View

How do I differentiate monocytes into a phenotype similar to Tumor-associated macrophages (for breast cancer)?

Would a good approach be to use supernatants from various breast cancer cell lines and differentiate these CD14+ monocytes from healthy donor blood into macrophages in their presence for 10-14...

01 January 1970 4,788 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View