Does anyone experienced EDTA salts precipitation in DMSO?

18 October 2020 1 7K Report

I added 2 mL of 0.1 M EDTA aqueous solution pH 6, to a solution of 3 mL pure DMSO and instantaneously I observed salt precipitation in solution. Is this happening because the pH of the EDTA aqueous solution is only 6?

Thanks in advance!

Nathan Ponzar

I think it's a combination of the decreased polarity of the solvent and the low pH. EDTA won't go into solution until you pH it to around 8, then you can drop it back down to 6, but it seems way less soluble at low pH. Then you suddenly decreased the polarity of the solvent, making it even less soluble.

So you need 0.04 M EDTA pH 6 in 60% DMSO?

Badges
Science topic

More Mónica Lucena's questions See All

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

Would anyone have a sample survey questionnaire for an AHP-TOPSIS study?

I am currently working on my undergraduate thesis project and we are trying to compare the AHP method with the AHP-TOPSIS method. I have already found the format for an AHP survey questionnaire,...

03 January 2021 7,213 3 View

Will -80 freezer cause damage to enzymes?

The typical guidance on storing enzymes is to keep them in a -20C freezer, but would storing aliquots of enzyme for long-term storage in a -80C freezer cause damage that would result in the enzyme...

03 December 2020 7,550 3 View

Did anyone try to measure the thickness of films with fluorescent peptides immobilized by ellipsometry?

I immobilize fluorescent peptide on chitosan films and when I try to measure the thickness of the film with ellipsometry, the software says that I have a film with 2 nm or less. However, with...

01 October 2020 3,702 3 View

Is sytematic review methodology adoped in demographic and population studies?

can a demographer employed the systematic review methodology in their research writing?

19 September 2020 5,242 4 View

How to run Targeted MD simulation using NAMD/ GROMACS?

I want to do targeted MD simulation on a protein (400 amino acids). Can anyone suggest me any good tutorial? Thanks in advance.

17 September 2020 342 2 View

Does anyone sell/know how to make 5 M sodium acetate solution?

I am trying out a new type of binding buffer for DNA extraction and the protocol calls for 5 M sodium acetate, but I cannot find anywhere that sells a stock solution that highly concentrated. The...

13 August 2020 5,149 5 View

Impact of digitalization on Mechanical Engineering?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

27 June 2020 2,350 13 View

Impact of data-driven industry and digitalization on Mechanical Engineering field?

I am currently researching the impacts of components of digital transformation and Industry 4.0 (optimization, automation, digitalization) on the fairly old field of mechanical engineering. Has...

24 June 2020 2,360 2 View

Whas is the ideal pathway for making an economic assesment for hidrologic ecosystem services?

17 April 2020 5,969 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

No title

22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View