How to separate solid NHS-folate during the folic acid activation?

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Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Sequence of an unknown Oligonucleotide ?

Hi All, I do have a single-stranded oligonucleotide of 100 - 150 bases synthesized and the sequence is not known. How would I proceed to know the sequence .

04 February 2021 5,614 3 View

In online GC-FID/TCD connect to flow reactor, why I am getting instable peak area in FID in every injection under same conditions?

We have online GC-FID and GC-TCD (Mod: 2014) connected with flow reactor system. This GC-FID/TCD is connected to a gas line coming from flow reactor system which I used for cascade reaction. In...

11 November 2020 4,999 6 View

Please suggest that the way of obtaining the concentration (in mol) from the gas mixture (N2=98%, Ethylene=1% and Butadiene=1%) is correct or not?

I have a gas mixture (N2=98%, Ethylene=1% and Butadiene=1%). I want to obtained the molar concentration of each components for 1mL volume of the gas mixture. For that purpose, I have used the...

29 September 2020 6,233 1 View

What should be the minimum and maximum dopant concentration to achieve best photocatalytic activity of any semiconductor?

I am working on modifying the band structure of TiO2 semiconductor for visible light photocatalytic activity. For this reason, I would like to dope the metal and/or nonmetal ions into the TiO2 to...

23 September 2020 1,063 6 View

How to calibrate a GC-FID where two of the components are gas phase & others are in liquid phase?

I am new in GC-FID and TCD measurement technique. I will analyze a gas stream at high temperature above 190 oC in the GC-FID system. I want to construct a calibration curve for the mixture of...

08 September 2020 6,073 3 View

Anybody has an experience on the DSSAT 4.7 model ? please let me know?

I am working on the DSSAT model, its a little bit harder than I think please help me?

04 May 2020 5,708 3 View

Why are the COVID-19 infection and mortality rates lower in India?

It is intriguing that the number of reported cases infected or died due to COVID-19 infection in India is comparatively much lower than the United States (US), China and other European countries.

18 April 2020 2,170 22 View

How to convert netcdf file in *.nc4 format to .csv or excel format containing data on planetary boundary layer height?

Monthly average data on Planetary boundary layer height is downloaded from disc.gsfc.nasa.gov. It is in netcdf file in *.nc4 format. How to convert this into .csv or excel format ?

15 December 2019 8,137 3 View

Antibody Immobilization by DTSSP ?

Hi all, I am trying to immobilize Antibody by DTSSP on a gold substrate, The DTSSP has a center disulfide. Most of the research articles have not reported the reducing of DTSSP...

26 November 2019 8,742 1 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View